Proposal of a pathway for enteric virus groups detection as indicators of faecal contamination to enhance the evaluation of microbiological quality in freshwater in Argentina

Masachessi, Gisela; Prez, Verónica Emilse; Michelena, J. F.; Lizasoain, Andrés; Ferreyra, Leonardo; Martı́nez, Laura C.; Giordano, Miguel O.; Barril, Patricia A.; Pavan, Jorge Victorio; Pisano, María Belén; Farías, Adrián; Isa, Marı́a Beatrı́z; Ré, Viviana Elizabeth; Colina, Rodney; Nates, Silvia

doi:10.1016/j.scitotenv.2020.143400

Cited by 8 publications

(4 citation statements)

References 66 publications

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“…The microbiological quality of these recreational surface waters was monitored by the determination of fecal indicator bacteria (FIB). Our results did not show a relationship between EV and RVA detection and bacterial contamination, in line with what has been previously proposed (Aguirre et al 2019, Masachessi et al 2021. Masachessi et al (2021) even proposed a pipeline involving the detection of picobirnavirus, human adenovirus and infectious EV in samples with FIB within acceptable limits by the guidelines as a way to improve the assessment of microbiological quality in freshwater, in addition to reporting the presence of other enteric viruses, such as RVA.…”

Section: Escherichia Colisupporting

confidence: 91%

“…These viruses cause several clinical manifestations, ranging from mild (respiratory and gastrointestinal infections, herpangina and hand-footand-mouth disease) to severe diseases (pleurodynia, hepatitis, myopericarditis, pancreatitis, meningitis, encephalitis, paralysis, and neonatal sepsis) (De Crom et al 2016). The EV is a known waterborne pathogen, with well documented spread in recreational water in both developed and developing countries and its use as a viral indicator of fecal contamination in these water matrices is also suggested (Allman et al 2013, Lee et al 2014, Maurer et al 2015, Sarmirova et al 2016, Aguirre et al 2019, Masachessi et al 2021.…”

Section: _________________ Corresponding Editor: María S Morales Cova...mentioning

confidence: 99%

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Two-year monitoring of enterovirus and rotavirus A in recreational freshwater from an island region, Pará State, northern Brazil

Alves

Teixeira

Deus

et al. 2022

Lat. Am. J. Aquat. Res.

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Enteric viruses are major causes of waterborne diseases and are present in large quantities in the stools of infected individuals. Its viability in the environment lasts for months, favoring the contamination of water used for consumption and recreation. The study aimed to monitor monthly the circulation of enterovirus (EV) and group A rotavirus (RVA) in recreational freshwater from an island region used as a bathhouse in northern Brazil, from January 2012 to December 2013. The viral RNA was obtained using guanidine isothiocyanate/silica after viral concentration by adsorption-elution method. The molecular detection was carried out by semi (EV) and nested-PCR (RVA) and the amplicons were sequenced on automated sequencer. At least one of these viruses was detected on 40.4% (42/104) of the samples. RVA was the most frequent (n = 32; 30.8%) when compared to EV (n = 20; 19.2%). Co-circulation between both was identified in 9.6% (n = 10). The highest viral positivity was found in SP02 (46.1%). The highest viral positivity was observed during high tides (57.7%; 60/104). Most EV samples were characterized as coxsackievirus (CV) A5 (85.7%, 12/14) and others as Sabin 1 poliovirus (14.2%, 2/14). The RVA positive samples were genotyped as G2, G3, G9, G12, P[8], P[4], and P[6]. These viruses were detected in 35.6% (37/104) of the samples with an acceptable concentration of fecal coliform bacteria. These results demonstrate the contamination of surface water intended for recreation by enteric viruses of Public Health concern even when bacterial indicators are within the tolerated limit, a factor that confirms the need for public policies aimed the sewage treatment before its release into water bodies.

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Section: Escherichia Colisupporting

confidence: 91%

Section: _________________ Corresponding Editor: María S Morales Cova...mentioning

confidence: 99%

Two-year monitoring of enterovirus and rotavirus A in recreational freshwater from an island region, Pará State, northern Brazil

Alves

Teixeira

Deus

et al. 2022

Lat. Am. J. Aquat. Res.

View full text Add to dashboard Cite

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“…It is well known that the microbiological contamination of surface water courses can potentially make these water resources an important source of dissemination of viral agents and of viral infection risk for people exposed to these waters [35,36]. The SARS-CoV-2 pandemic continues to raise various questions about its infective potential in the different watercourses where it is found, particularly in freshwater sources that are affected by poorly treated or untreated wastewater, and in those water resources that serve as key health spaces for the recovery of patients affected by diseases [11].…”

Section: Infective Capacitymentioning

confidence: 99%

A Preliminary Study of SARS-CoV-2’s Permanence and Potential Infective Capacity in Mineromedicinal Waters of Copahue, Neuquén, Argentina

Vela

Masachessi

Giaveno

et al. 2022

IJERPH

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Copahue Thermal Center is characterized by the presence of mineromedicinal acidic waters with high temperatures, therapeutic peloids, and relevant consortia of extremophiles species, distributed in small natural pools which cannot be disinfected. The objective of this research was to investigate the survival of SARS-CoV-2 in Copahue’s waters and its remaining infective capacity. In a first assay, a decrease of more than 50% of the initially viral load compared to the initially inoculated positive sample was detected for all the water samples analyzed. After that, two of the Copahue springs, which are used as an immersion bath in closed environments without going through any disinfection treatment, was selected to determine the viral viability. VERO cell infections were performed, with no cytopathic effect detected, but a strikingly high resistance of the virus, detecting its genome by real time PCR, during the seven days of study under laboratory conditions. SARS-CoV-2 survival in acid media was reaffirmed, which is a peculiarity for a covered virus. A decrease in the detectable viral load of the positive sample was found as the infection time passed, becoming completely negative in the subsequent blind passages. More research is needed to further study the feasibility of SARS-CoV-2 in mineromedicinal waters, especially natural acidic waters that cannot disinfected, in order to expand information about the risk to populations that are exposed to them.

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“…Enteric viruses, such as Human mastadenovirus (HAdV) and Enterovirus (EV), are important waterborne pathogens that have the potential to indicate fecal contamination and, thus, can be used as complementary indicators to improve the assessment of microbiological water quality (Masachessi et al, 2021;Zhu et al, 2018). They have been detected in domestic wastewater and various types of drinking water sources worldwide and make up the Contaminant Candidate List 4 of the United States Environmental Protection Agency (USEPA), which brings together contaminants that, despite having a high prevalence in fecally contaminated water, are not regulated as water quality analysis parameters (La Rosa et al, 2012;U.S.…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli, Species C Human Adenovirus, and Enterovirus in Water Samples Consumed in Rural Areas of Goiás, Brazil

et al. 2021

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Rural environments lack basic sanitation services. Facilities for obtaining water and disposing sewage are often under the initiative of each resident, who may not be able to build and maintain them properly. Thus, water for human consumption is subject to fecal contamination and, consequently, the presence of waterborne pathogens, such as enteric viruses. This study evaluated fecal contamination of water samples from individual sources used for domestic water supply on small farms in the state of Goiás, Brazil. Samples were collected from 78 houses whose water sources were tubular wells, dug wells, springs, and surface waters. Escherichia coli (EC) bacteria, analyzed by the defined chromogenic substrate method, was used as a traditional indicator of fecal contamination. The enteric viruses Human mastadenovirus (HAdV) and Enterovirus (EV), analyzed by qPCR, were tested as complementary indicators of fecal contamination. At least one of these markers was found in 89.7% of the samples. Detection rates were 79.5% for EC, 52.6% for HAdV, and 5.1% for EV. The average concentration for EC was 8.82 × 10 1 most probable number (MPN) per 100 mL, while for HAdV and EV the concentrations were 7.51 × 10 5 and 1.89 × 10 6 genomic copies (GC) per liter, respectively. EC was the most frequent marker in ground and surface water samples. HAdV was detected significantly more frequently in groundwater than in surface water and was more efficient in indicating contamination in tubular wells. There was no association of frequencies or correlation of concentrations between EC and HAdV. HAdV indicated human fecal contamination and performed well as a complementary indicator. The results reveal that a large part of the analyzed population is vulnerable to waterborne diseases caused by enteric pathogens.

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Proposal of a pathway for enteric virus groups detection as indicators of faecal contamination to enhance the evaluation of microbiological quality in freshwater in Argentina

Cited by 8 publications

References 66 publications

Two-year monitoring of enterovirus and rotavirus A in recreational freshwater from an island region, Pará State, northern Brazil

Two-year monitoring of enterovirus and rotavirus A in recreational freshwater from an island region, Pará State, northern Brazil

A Preliminary Study of SARS-CoV-2’s Permanence and Potential Infective Capacity in Mineromedicinal Waters of Copahue, Neuquén, Argentina

Escherichia coli, Species C Human Adenovirus, and Enterovirus in Water Samples Consumed in Rural Areas of Goiás, Brazil

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