Propofol Protects against Ischemia/Reperfusion Injury Associated with Reduced Apoptosis in Rat Liver

Abstract

Propofol is an intravenous anesthetic, reported to have a protective effect against ischemia/reperfusion (I/R) injury in heart and brain, but no definite data are available concerning its effect in hepatic I/R. This work investigated the effect of propofol anesthesia on hepatic I/R injury using in vivo rat model. Four groups of rats were included: sham operated, I/R (30 min ischemia and 2 h reperfusion), I/R treated with propofol (10 mg/kg/h), and I/R treated with propofol (20 mg/kg/h). Liver enzyme leakage, TNFand caspase-3 levels, and antiapoptotic Bcl-xL/apoptotic Bax gene expression, together with histopathological changes, were used to evaluate the extent of hepatic I/R injury. Compared with sham-operated group, I/R group showed significant increase in serum levels of liver enzymes (ALT, AST), TNF-, and caspase-3 and significant decrease in the Bcl-xL/Bax ratio, associated with histopathologic damage in liver. Propofol infusion significantly attenuated these changes with reduced hepatic histopathologic lesions compared with nonpreconditioned I/R group. However, no significant differences were found between two groups treated with different doses of propofol. In conclusion, propofol infusion reduced hepatic I/R injury with decreased markers of cellular apoptosis. Therefore, propofol anesthesia may provide a useful hepatic protection during liver surgery.

Propofol attenuates cytokine-mediated upregulation of expression of inducible nitric oxide synthase and apoptosis during regeneration post-partial hepatectomy

“…The TNF-α level in rats that underwent PHT under propofol anesthesia was significantly lower than in those that underwent PHT under ketamine anesthesia, suggesting that propofol might have a protective effect on the liver. These findings are in agreement with those of Abdel-Wahab et al 17 , who reported attenuation of the TNF-α level and expression of apoptotic genes upon propofol infusion in ischemic livers.…”

Curcumin nanoparticles loaded hydrogels protects against aflatoxin B1-induced genotoxicity in rat liver

“…The mRNA expression of Bax, Bcl-2 and Caspase-3 was determined to evaluate whether AFB 1 -induce apoptosis in rat liver is related to the mitochondrial pathway. The increased in mRNA expression of Bax and Caspase-3 and the decreased in mRNA expression of Bcl-2 and the ratio of Bcl-2/Bax in hepatocytes of rats treated with AFB 1 suggest that the excessive apoptosis of hepatocytes induced by AFB 1 was onset by the mitochondrial signaling pathway (Abdel-Wahab and Al-Harizy, 2013;Abdel-Aziem et al, 2014). It was demonstrated also that AFB 1 could lead to cellular apoptosis via mitochondrial or cell death receptor pathways (Abdel-Aziem et al, 2011;Peng et al, 2014).…”

Vildagliptin Attenuates Hepatic Ischemia/Reperfusion Injury via the TLR4/NF‐κB Signaling Pathway

“…The ischemic liver is characterized by cellular apoptosis and programmed cell death, which is recognized by the caspase family in which caspase-3 is the executive caspase [ 67 ]. Growing evidence suggested that TNF- α and oxidative stress played a crucial role in magnifying the apoptosis in the ischemic liver [ 23 , 36 ].…”

“…The HIR injury was performed as described in previous studies [ 22 , 23 ]. Rats were anesthetized with a mixture of ip 80 mg/kg ketamine (Ketamax-50, Troikaa Pharmaceuticals Ltd., Gujarat, India) and 10 mg/kg xylazine (Xyla-Ject®, Adwia Co., 10th of Ramadan, Egypt) [ 24 , 25 ].…”