Propofol Attenuates Peroxynitrite-mediated DNA Damage and Apoptosis in Cultured Astrocytes

Acquaviva, Rosaria; Campisi, A.; Murabito, Paolo; Raciti, G.; Avola, Roberto; Mangiameli, S; Musumeci, Ilenia; Barcellona, Maria Luisa; Vanella, A.; Volti, Giovanni Li

doi:10.1097/00000542-200412000-00017

Cited by 91 publications

(65 citation statements)

References 27 publications

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“…Indeed, propofol has been shown to significantly increase heme oxygenase production in astrocytes and astroglial cells. 27,28 Heme oxygenase is a molecule with antioxidant properties that has been demonstrated to play a critical role in intestinal ischemic preconditioning that mediates protection against intestinal mucosal injury and the subsequent systemic inflammatory response. 29,30 Therefore, propofol may have initiated a preconditioning-like effect that is characterized by an increase of the endogenous antioxidant defenses, such as the increase of heme oxygenase and SOD activities.…”

Section: Discussionmentioning

confidence: 99%

Propofol attenuates intestinal mucosa injury induced by intestinal ischemia-reperfusion in the rat

Liu

Rinne

et al. 2007

Can J Anesth/J Can Anesth

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Purpose:We investigated whether propofol at a sedative dose can prevent intestinal mucosa ischemia/reperfusion (I/R) injury, and if propofol can attenuate oxidative stress and increases in nitric oxide (NO) and endothelin-1 (ET-1) release that may occur during intestinal I/R injury. Methods:Rats were randomly allocated into one of five groups (n = 10 each): (i) sham control; (ii) injury (one hour superior mesenteric artery occlusion followed by three hours reperfusion); (iii) propofol pre-treatment, with propofol given 30 min before inducing intestinal ischemia; (iv) simultaneous propofol treatment, with propofol given 30 min before intestinal reperfusion was started; (v) propofol post-treatment, with propofol given 30 min after intestinal reperfusion was initiated. In the treatment groups, propofol 50 mg·kg -1 was administrated intraperitoneally. Animals in the control and untreated injury groups received equal volumes of intralipid (the vehicle solution of propofol) intraperitoneally. Intestinal mucosa histology was analyzed by Chiu's scoring assessment. Levels of lactic acid (LD), NO, ET-1, lipid peroxidation product malondialdehyde (MDA) and superoxide dismutase (SOD) activity in intestinal mucosa were determined. Results:Histological results showed severe damage in the intestinal mucosa of the injury group accompanied by increases in MDA, NO and ET-1 and a decrease in SOD activity. Propofol treatments, especially pre-treatment, significantly reduced Chiu's scores and levels of MDA, NO, ET-1 and LD, while restoring SOD activity. Conclusion:These findings indicate that propofol attenuates intestinal I/R-induced mucosal injury in an animal model. The response may be attributable to propofol's antioxidant properties, and the effects of inhibiting over-production of NO and in decreasing ET-1 levels. CAN J ANESTH 2007 / 54: 5 / pp 366-374 Objectif : Nous avons cherché à savoir si le propofol, en dose sédative, pouvait empêcher les lésions d'ischémie/reperfusion (I/R) de la muqueuse intestinale, et s'il pouvait atténuer le stress oxydatif et les augmentations dans la libération d'oxyde nitrique (NO) et d'endothéline-1 (ET-1) pouvant survenir lors de lésions I/R intestinales. Méthode : Des rats ont été randomisés en cinq groupes (n = 10 chacun) : (i) faux témoin (sham control) ; (ii) lésion (occlusion de

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Section: Discussionmentioning

confidence: 99%

Propofol attenuates intestinal mucosa injury induced by intestinal ischemia-reperfusion in the rat

Liu

Rinne

et al. 2007

Can J Anesth/J Can Anesth

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“…To monitor cell viability, the cells were plated at 8x10 3 cells per well of a 96-multiwell flat-bottomed 200 µl microplate (24). The optical density of each well sample was measured with a microplate spectrophotometer reader (Titertek Multiskan, Flow Laboratories, Helsinki, Finland) at λ=570 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Lactate dehydrogenase (LDH) release was measured to evaluate the presence of cell necrosis as a result of cell disruption, subsequent to membrane rupture. LDH activity was measured spectrophotometrically in the culture medium and in the cellular lysates, at λ=340 nm by analyzing NADH consumption (24).…”

Section: Methodsmentioning

confidence: 99%

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Antiproliferative effect of oleuropein in prostate cell lines

Vanella

2012

Int J Oncol

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Abstract. Currently, there is increasing interest in the in vivo protective effects of natural antioxidants found in dietary plants against oxidative damage caused by free radical species. Oxidative stress has been invoked as a causative agent in cancer and epidemiological data suggest that the consumption of fruits and vegetables may be associated with a lower incidence of cancer. The fruit of the Olea europaea L. and olive oil contain hundreds of phytochemicals and its extracts have recently been shown to exhibit antioxidant properties, due to the action of oleuropein. In view of these considerations, in this study, we investigated the effects of oleuropein on LNCaP and DU145 prostate cancer cell lines and on BPH-1 non-malignant cells. Oleuropein reduces cell viability and induces thiol group modifications, γ-glutamylcysteine synthetase, reactive oxygen species, pAkt and heme oxygenase-1. Exposing cell cultures to oleuropein induces an antioxidant effect on BPH-1 cells and a pro-oxidant effect on cancer cells. Our results confirm the beneficial properties of olive oil and oleuropein, suggesting its possible use as an adjuvant agent in the treatment of prostatitis, in order to prevent the transformation of hypertrophic to cancerous cells.

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“…Isoflurane (ISF) and sevoflurane are the most used inhaled halogenated anaesthetics. The introduction of ISF (2-chloro-2-(difluoromethoxy)-1,1,1-trifluoro-ethane) in clinical practice has represented an advance for inhalation anaesthesia, due to its low metabolism rate and low blood-gas partition coefficient, which decreases its induction and recovery times (6). The ISF is metabolized by CYP2E1 enzyme, generating an intermediate that can decompose into reactive metabolites, or the intermediate trifluoroacetyl ester.…”

Section: Introductionmentioning

confidence: 99%

Potencial toxicogenômico e citotóxico dos anestésicos propofol e isoflurano em indivíduos submetidos a procedimentos cirúrgicos

Braz¹

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O meu eterno agradecimento aos meus pais José Reinaldo e Fátima, pelo amor incondicional, educação, incentivo, apoio, carinho e fé. Amo vocês.Aos meus queridos irmãos Leandro, Fabiana e Danilo, e minha cunhada Rúbia, por me incentivarem e estarem sempre dividindo todos os momentos. Tenho carinho muito especial por vocês.Aos meus tios, tias e primos, obrigada pelo carinho.Aos fofos Neon e Nenê por existirem.Dedico este trabalho à minha querida família e aos meus amigos. À Juliana Giacobino, Por ter me ajudado nos experimentos e análises do teste do cometa. À Juliana Capannacci e Adriana Ferrasi,Pela disponibilidade e ajuda na área de biologia molecular.Ao meu pai e ao meu irmão Leandro, Pelo empenho e dedicação, e pelas coletas realizadas.A todos os pacientes, que doaram seu sangue pela ciência.Aos docentes e residentes do Departamento de Anestesiologia da FMB-UNESP, em especial, os residentes Eduardo Sakai e Matheus Pinotti, pela dedicação e entrevistas com os pacientes.Aos Drs José Vicente Tagliarini e Norimar Dias, do Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço da FMB-UNESP, pelas cirurgias realizadas. Às colegas e funcionárias Márjorie Golim e Valéria da Silva, do Laboratório de Citometria deFluxo do Hemocentro da FMB-UNESP, pela compreensão e convivência.Às funcionárias e colegas Dra Paula Hokama, Elizabethe Garcia, Rejane Groto, Camila Verdichio, Patrícia Levada, Chiara Legnaro e Juliana Padovani, do Laboratório de Biologia Molecular do Hemocentro da FMB-UNESP, pela agradável convivência. À Profa Lídia de Carvalho e ao doutorando João Paulo Marcondes, pelas análises estatísticas. À Selma de Jesus e Rosemary da Silva, bibliotecárias da UNESP-Botucatu, pela realização da ficha catalográfica e orientação das referências, respectivamente.À Janete Silva, Andrea Devidé, Lílian Nunes, Regina Spadin e Nathanael Salles, da seção de Pós-Graduação, e à Vânia Soler, secretária da Pós-Graduação em Patologia, da FMB-UNESP, pelo apoio e por sempre estarem dispostos a ajudar. Aos funcionários e colegas que convivi do Laboratório TOXICAM: Paulo, Mara, Carla, Tony, Merielen, Alexandre, Meire, João, Marize, Viviane, Bruno, Ana Paula, Gabrielli, Bianca, e a todos os pós-graduandos da Patologia, pelo convívio.Aos colegas e amigos do Laboratório de Toxicogenômica e Nutrigenômica: Juliana Dorico, Camila Gobette, Kamila Kihara, Luciana Feliciano, Magaly Monteiro, Rodrigo de Lima, Danielle de Almeida; Renato Prado, Elaine de Camargo, João Paulo Marcondes e Glenda da Silva, pela convivência, aprendizagem, companheirismo; dificuldades compartilhadas, conquistas, congressos e viagens, e à amizade sincera. Vocês são especiais. À Shadia Ihlaseh,Pela amizade e ensinamentos, convivência nos EUA e ajuda nas análises de expressão gênica.Aos colegas e amigos de Botucatu, e da faculdade (XXXVI turma de Biomedicina-UNESPBotucatu): Juliana dos Santos, Stella Freitas, Lina Ito, Mariana Greatti, Thaís Carvalho, Nahomi Yamaki, Priscilla Negraes, Renata da Silva; e em especial, Willian Luna, Flávia Chaves, Fern...

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Propofol Attenuates Peroxynitrite-mediated DNA Damage and Apoptosis in Cultured Astrocytes

Abstract: The antioxidant properties of propofol can be partially attributed to its scavenging effect on peroxynitrite as well as to its ability to increase HO-1 expression at higher concentrations, a property that might be relevant to neuroprotection during anesthesia.

Cited by 91 publications

References 27 publications

Propofol attenuates intestinal mucosa injury induced by intestinal ischemia-reperfusion in the rat

Propofol attenuates intestinal mucosa injury induced by intestinal ischemia-reperfusion in the rat

Antiproliferative effect of oleuropein in prostate cell lines

Potencial toxicogenômico e citotóxico dos anestésicos propofol e isoflurano em indivíduos submetidos a procedimentos cirúrgicos

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