Propionibacterium microaerophilum sp. nov., a microaerophilic bacterium isolated from olive mill wastewater.

Abstract

A new Gram-positive, facultative anaerobic, microaerophilic bacterium, designated strain M5 T , was isolated from a decantation reservoir of olive mill wastewater. The cells were rod-shaped, non-motile, non-spore-forming and catalase-negative. Growth occurred at pH ranging from 45 to 95, with optimum growth at 70. The optimum temperature for growth was around 30 SC. Although growth occurred under anaerobic and aerobic conditions, the optimum O 2 concentration for growth was determined as 5 % in the gas phase of the culture. During anaerobic growth, glucose or lactate were mainly fermented to propionate, acetate and CO 2 . In the presence of O 2 (more than 2 %), glucose was oxidized completely to CO 2 . The GMC content of the DNA was 677O06 mol % and 16S rRNA gene analysis revealed that the new isolate belonged to the cluster of ' dairy ' propionibacteria, Propionibacterium acidipropionici being its closest phylogenic relative (975 % similarity). However, the level of DNA relatedness between strain M5 T and P. acidipropionici was 562 %. Consequently, both the phenotypic (range of substrates used) and genotypic characteristics of strain M5T allow it to be assigned as a new species of the genus Propionibacterium, Propionibacterium microaerophilum sp. nov. The type strain is strain M5 T (l CNCM I-2360 T l DSM 13435 T ).

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

“…In the last two decades six new species were isolated: P. cyclohexanicum was obtained from spoiled orange juice [10]; P. microaerophilum was isolated from olive mill wastewater [11]; P.…”

Prevalence of the Genes Encoding Propionicin T1 andProtease-Activated Antimicrobial Peptide and Their Expressionin ClassicalPropionibacteria

“…Phylogenetic studies have shown that P. jensenii and P. thoenii are more closely related than other dairy propionibacteria (4,13). The strains harboring the pctA and pamA genes were obtained from different environments in Europe, the United States, and South Africa, indicating that the ability to produce these antimicrobial compounds is widely distributed in the two species.…”

Application of 16S-ARDRA and RFLP-PFGE for improved genotypic characterisation of dairy propionibacteria and combination with characteristic phenotypes

“…According to the sequence of 16S rRNA, new Propionibacterium species isolated from other biotopes have exhibited similarity to the dairy propionibacteria. Propionibacterium cyclohexanicum (Kusano, Yamada, Niwa, & Yamasato, 1997) was shown to be phylogenetically related to P. freudenreichii, whereas Propionibacterium microaerophilum (Koussemon et al, 2001) was shown to be related to P. acidipropionici.…”