Propionate and butyrate metabolism in rat or sheep hepatocytes

Demigné, Christian; Yacoub, C.; Rémésy, Christian; Fafournoux, Pierre

doi:10.1016/0005-2760(86)90074-3

Cited by 51 publications

(23 citation statements)

References 41 publications

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“…SCFA are rapidly absorbed by the colonocytes. While butyrate is mainly metabolized by these cells [30], acetate and propionate reach the portal vein blood, which transports them to the liver [31]. In agreement with previous reports, we could show that cecal acetate and propionate concentrations correlate with portal vein concentrations [32].…”

Section: Discussionsupporting

confidence: 92%

Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice

Weitkunat

Schumann

Petzke

et al. 2015

The Journal of Nutritional Biochemistry

173

142

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Section: Discussionsupporting

confidence: 92%

Effects of dietary inulin on bacterial growth, short-chain fatty acid production and hepatic lipid metabolism in gnotobiotic mice

Weitkunat

Schumann

Petzke

et al. 2015

The Journal of Nutritional Biochemistry

173

142

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“…Propionate is used in these experiments because it is nearly as effective as butyrate in inhibiting histone deacetylation (McKnight et al, 19801, but has an effect opposite to that of butyrate on glycolysis, lactate formation, and pH changes in hepatocyte cultures (Anderson and Rridges, 1984), thus emphasizing the importance of its effect on histone modification. Propionate has also been reported to be metabolized more slowly than butyrate by cultured rat hepatocytes (Demigne et al, 1986). The results shown in Table 1 agree with a previous report from our laboratory that poly(ADP)ribose-synthetase inhibitors lower the GGT expression by cultured hepatocytes (Althaus et al, 1982131, but the addition of dexameth- asone has little effect on GGT activity in this culture system (Sirica et al, 1979, results not shown).…”

Section: Resultssupporting

confidence: 92%

Sodium butyrate preserves aspects of the differentiated phenotype of normal adult rat hepatocytes in culture

Staecker

Sattler

Pitot

1988

Journal Cellular Physiology

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We have determined that sodium butyrate and, to a lesser extent, dimethylsulfoxide (DMSO) and 3-aminobenzamide (3-AB) preserve aspects of the differentiated phenotype of primary cultures of adult rat hepatocytes. The histone deacetylase inhibitor, butyrate, inhibits the increase in gamma-glutamyltranspeptidase (GGT) activity and the decrease in basal tyrosine aminotransferase (TAT) activity normally observed when hepatocytes are cultured under appropriate conditions. The effects of butyrate on GGT and TAT activities are accompanied by parallel changes in GGT and TAT mRNA levels. The poly(ADP)ribose-synthetase inhibitor, 3-aminobenzamide, has effects similar to butyrate on GGT activity and mRNA levels, while both 3-AB and DMSO increase basal TAT activity in cultured hepatocytes. Under appropriate conditions all three agents--butyrate, 3-AB, and DMSO--extend the length of time cultured hepatocytes can be maintained as confluent monolayers. However, under all the conditions studied, butyrate extended the length of time hepatocytes could be maintained as monolayers more than any other treatment used. Butyrate-treated hepatocytes maintained ultrastructural features that were more similar to those of hepatocytes in vivo than hepatocytes treated with any other of the agents tested. Histone acetylation levels of primary cultures of adult rat hepatocytes declined concomitant with the loss of the differentiated phenotype of the cells. These results suggest that histone acetylation may play a role in the changes in gene expression observed when hepatocytes are placed in culture.

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“…The contribution of propionate to glucose production in ruminants is about 60% (Demigné et al, 1986), but factors affecting the efficiency of conversion of propionate to glucose in ruminants are still unclear. The increase in glycemia during the PLT (Table 1, Fig.…”

Section: Discussionmentioning

confidence: 99%