Prophage Induction in Lysogenic Escherichia coli with N-Nitroso Compounds and Derivatives

Abstract

Prophage induction in lysogenic Escherichia coli W1709 (X) was determined for 29 N-nitroso compounds, 13 of their denitrosated derivatives, and 7 hydroxylamino and hydrazino analogues of nitrosamines. Minimal inducing concentrations of 0.1 to 2.0 gtg/ml were demonstrated for eight nitrosamidines, and concentrations of 0.5 to 25.0 pg/ml were shown for six nitrosamides. Weak in

Prophage Induction in Escherichia coli (Lambda) by N-Nitrosamines

“…Many N-nitroso compounds have been shown to be both carcinogens and inducers. However, N-nitrosamines, which are among the most potent carcinogens known 7, have not been shown to have inducing abilities (4). The present study was undertaken in an attempt to establish an induction system sensitive to N-nitrosamines.…”

“…An ever-increasing wealth of evidence supports the correlation between the ability of a substance to induce lysogenic bacteria and its carcinogenic activities (2,4,5). Although not all carcinogens or antineoplastic agents tested have been shown to be inducers (9), the correlation is such that induction can be used as a rapid in vitro screening system for potentially harmful carcinogens and useful antineoplastic compounds (3).…”