Prophage Induction in <i>Escherichia Coli</i> K-12(λ) by Some Plants from Iran

Taghvaei, Mohammad; Naghibi, Farzaneh; Mosaddegh, Mahmoud; Moazzami, Nasrin; Ghorbani, Abdolbaset; Fakhari, Ali Reza

doi:10.1080/09735070.2009.11886338

Cited by 5 publications

(1 citation statement)

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“…The Boraginaceae is a large family that comprises approximately 205 genera and 2500 species worldwide [ 41 ]. The root extract of C. macranthera was studied for induction of phage production [ 42 ]. Different chemical constituents including glycosides [ 43 ], pyrrolizidine alkaloids [ 44 ], triterpenoid sapogenin [ 45 ] have been reported from the species of the genus Caccinia other than C. macranthera .…”

Section: Introductionmentioning

confidence: 99%

Antitumor evaluation of two selected Pakistani plant extracts on human bone and breast cancer cell lines

Engel

Ali

Adamus

et al. 2016

BMC Complement Altern Med

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BackgroundThe medicinal plants Vincetoxicum arnottianum (VSM), Berberis orthobotrys (BORM), Onosma hispida (OHRM and OHAM) and Caccinia macranthera (CMM) are used traditionally in Pakistan and around the world for the treatment of various diseases including cancer, dermal infections, uterine tumor, wounds etc. The present study focuses on the investigation of the selected Pakistani plants for their potential as anticancer agents on human bone and breast cancer cell lines in comparison with non-tumorigenic control cells.MethodsThe antitumor evaluation was carried out on human bone (MG-63, Saos-2) and breast cancer cell lines (MCF-7, BT-20) in contrast to non-tumorigenic control cells (POB, MCF-12A) via cell viability measurements, cell cycle analysis, Annexin V/PI staining, microscopy based methods as well as migration/invasion determination, metabolic live cell monitoring and western blotting.ResultsAfter the first initial screening of the plant extracts, two extracts (BORM, VSM) revealed the highest potential with regard to its antitumor activity. Both extracts caused a significant reduction of cell viability in the breast and bone cancer cells in a concentration dependent manner. The effect of VSM is achieved primarily by inducing a G2/M arrest in the cell cycle and the stabilization of the actin stress fibers leading to reduced cell motility. By contrast BORM’s cytotoxic properties were caused through the lysosomal-mediated cell death pathway indicated by an upregulation of Bcl-2 expression.ConclusionsThe antitumor evaluation of certain medicinal plants presented in this study identified the methanolic root extract of Berberis orthobotrys and the methanolic extract of Vincetoxicum arnottianum as promising sources for exhibiting the antitumor activity. Therefore, the indigenous use of the herbal remedies for the treatment of cancer and cancer-related diseases has a scientific basis. Moreover, the present study provides a base for phytochemical investigation of the plant extracts.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1215-9) contains supplementary material, which is available to authorized users.

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