Properties of β-Galactosidase from Lactobacillus salivarius subsp. salivarius Nam27

β‐Galactosidase fromLactobacillus pentosus: Purification, characterization and formation of galacto‐oligosaccharides

“…This pH optimum is slightly more alkaline than reported for most other lactobacillal βgalactosidases, which show an optimum of 6.5-7.0 [9,34]; however, we observed a pronounced effect of the buffer system used on the optimal pH, and that certainly affected the actual value determined. The temperature optimum of L. pentosus β-gal of 60-65°C is considerably higher than reported for most other β-galactosidases from lactobacilli, which are typically optimally active in the range of 45-50°C [9,34,43,50]. L. pentosus KUB-ST10-1 can grow at temperatures of up to 45°C.…”

“…In contrast to this, β-galactosidase from L. salivarius was reported to be a monomer of ca. 30 kDa [43], from L. delbrueckii subsp. bulgaricus a homodimer of 220 kDa [44], and from L. helveticus a homotetramer of 257 kDa [45]; L. plantarum forms, in addition to the above-mentioned heterodimeric enzyme, a homohexameric βgalactosidase of ca.…”