β‐Galactosidase fromLactobacillus pentosus: Purification, characterization and formation of galacto‐oligosaccharides

Abstract

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Galactooligosaccharide Production from Pantoea anthophila Strains Isolated from “Tejuino”, a Mexican Traditional Fermented Beverage

“…Based on the detailed analysis of the carbohydrate mixture profile, the permeabilized cells of P. anthophila strains display a high preference for the formation of galactosyl β(1 → 6) and β(1 → 3) linkages. This result was similar to that obtained with β-galactosidases from LAB [30][31][32]39].…”

“…After this point, hydrolysis prevailed over trangalactosylation, and the total GOS concentration was progressively decreased until lactose conversion was around 100% [8]. This GOS yield is comparable with other reported bacterial β-galactosidases, 38% for β-galactosidase from Lactobacillus reuteri [30], 31% for β-galactosidase from Lactobacillus pentosus [31], 41% for β-galactosidase from Lactobacillus sakei [32], and 41% for β-galactosidase from Bacillus circulans [33]. In the literature, the typical yields are between 30% and 40% (w/w).…”

Microbial production of prebiotic oligosaccharides

“…For example, the β-galactosidase from K. lactis produced predominantly β-(1→6)-linked GOS (Asp et al, 1980), the β-galactosidase from Aspergillus oryzae produced predominantly β-(1→3) and β-(1→6) linkages in their GOS (Toba et al, 1985), Bacillus circulans β-galactosidase forms β-(1→2), β-(1→3), β-(1→4), β-(1→6) linked GOS (Yanahira et al, 1995), whereas β-galactosidases from Lactobacillus spp. showed a preference to form β-(1→3) and β-(1→6) linkages in transgalactosylation mode Iqbal et al, 2010Iqbal et al, , 2011Nguyen et al, 2007;Maischberger et al, 2010).…”

Galacto-oligosaccharides and colorectal cancer: Feeding our intestinal probiome

“…β-Galactosidases have been isolated and characterized from a number of Lactobacillus species, including L. pentosus [79] , L. sakei [59] , L. delbrueckii subsp. bulgaricus [103] , L. plantarum [60] , L. reuteri [102] and L. acidophilus [101] .…”