Properties of the Mls System: A Revised Formulation of Mls Genetics and an Analysis of T‐Cell recognition of Mls Determinants

Ryo, Akihide; Hodes, Richard J.

doi:10.1111/j.1600-065x.1989.tb00001.x

Cited by 19 publications

(7 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VB20 was amplified with KgDO (complementary to positions 472-488 of V1520) and XNSC10. V~20-specific PCtLs were done with KguP (positions 241-256 of VB20) and MTB primers, of TCR B transcripts with the MCTBUP (positions [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] and an equimolar mixture of the MTB1DO and MTB2DO primers (complementary to positions 474-493 of C131 and C132), of TCR c~ transcripts with MTCAUP (positions [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] and MTCADO2 primers (complementary to positions 263-279), and of V1317 with MVB17S and MVB17FX, as previously described (10).…”

Section: Methodsmentioning

confidence: 99%

“…For example, V1317 and V/319 are found as pseudogenes in TCR B b haplotype and functional in TCR/3 ~ haplotype (3,4), and several strains display genomic deletions that remove up to 60% of their V/3s (5)(6)(7). Along with this variability of the germ-line repertoire, the usage of V/3s by mature T lymphocytes depends on MHC products and on the expression of superantigens that eliminate T cells bearing particular V/3s (8). The identification of the V/3s was largely based on screening of thymus or T cell clone cDNA libraries, therefore greatly depending on the frequency of V/3 usage, or on probing of genomic clones with consensus V/3 oligonucleotides that may miss V/3s differing in the region corresponding to the consensus primers.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice.

Six

Jouvin‐Marche

Loh

et al. 1991

The Journal of Experimental Medicine

View full text Add to dashboard Cite

SummaryA cDNA library of TCR/3 chain transcripts from BALB/c thymocytes was constructed using anchored polymerase chain reaction (PCR). Screening of this library led to the identification of a VB gene segment, V/320, structurally related to V/33 and V1317. Genomic analysis of mice displaying deletions in their V/3 loci, together with mapping of cosmid clones, situated V/320 2.5 kb beside VB17. The expression of VB20 was estimated by PCR in mice of different H-2 and Mls types. Peripheral T cells from H-2 k and H-2 d mice did not express V/320, whereas in I-E-negative mice (C57B1/6 and SJL), V/320 transcripts were detected. The lack of V/320 transcripts in (C57B1/6 x CBA/J)F1, (C57B1/6 x BALB/c)F1, and in congenic B6.H-2 k mice suggests that the differential use of V/320 is due to an I-E-mediated donal deletion process. The involvement of the Mls super antigens was excluded by analysis of all Mls type combinations. The nature of the V/B20-deleting element(s) is discussed in the context of the I-EAuperantigen systems controlling the expression of V/311 and VB17.

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice.

Six

Jouvin‐Marche

Loh

et al. 1991

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…At the same time analysis of the minor lymphocyte stimulatory (Mls) system provided insights into general aspects of T-cell recognition and repertoire generation (reviews in Abe and Hodes, 1989;Festenstein et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Regulatory T cells in the establishment and maintenance of self-tolerance: role of the thymic epithelium

Salaün¹,

Corbel²,

LeDouarin³

2005

Int. J. Dev. Biol.

View full text Add to dashboard Cite

The thymus constitutes the microenvironment for T lymphocyte differentiation and acquisition of self-tolerance. Aiming to specify the contributions of the two essential parts of the thymus, namely hemopoietic and epithelial, we have devised experimental models in birds and mice. Chimeric thymuses, xenogeneic in birds and allogeneic in mice, were constructed early in development. In both models we could demonstrate a critical role of the epithelial component of the thymic stroma in induction and maintenance of self-tolerance. These experiments showed that suppression mechanisms are also implicated in these events, strongly suggesting the existence of regulatory T cells in both models. Before these experiments the control of selftolerance was usually attributed to suppressive cells. However, as the cell phenotypes were not identified, the role of these cells was disregarded. Numerous studies since our investigations argue in favour of regulatory mechanisms. The work we initiated several years ago represents a contribution to our understanding of the two linked and opposite aspects of immune-responded control, namely self-tolerance and autoimmunity.

show abstract

“…Since it has been shown that Mlsa and Mls°determinants are encoded by nonallelic, unlinked loci based on segregation analysis (22), a new nomenclature has been proposed in which Mls-1 would represent the locus encoding Mlsa and Mls-2 would encode what was previously known as Mlsc (26)(27)(28). The findings described above indicate the existence of a third locus (Mls-3) that is involved in Mlsc expression (25,29). However, since the loci encoding Mls`determinants have not yet been mapped, and since it appears that two loci may encode a common determinant, we would suggest that Festenstein's original nomenclature (9) be maintained for Mls determinants as well as for Mls phenotypes Mlsa, Mlsb, and Mls`; Mlsd is now widely accepted to represent coexpression of Mlsa and Mls`determinants and can be discarded from use.…”

Section: Discussionmentioning

confidence: 92%

Analysis of Mlsc genetics. A novel instance of genetic redundancy.

Ryo

Foo-Phillips

Hodes

1989

The Journal of Experimental Medicine

View full text Add to dashboard Cite

The identity of the self determinants involved in the selection of the T cell repertoire has been a matter of considerable interest. In addition to the apparent critical role of MHC gene products, accumulated experimental results indicate the importance of non-MHC gene products in T cell repertoire selection. In particular, murine Mlsa and Mlsc determinants have been shown to be highly stimulatory to allogeneic T cells and to be involved in the negative selection (elimination) of self-reactive T cells expressing selected TCR V beta segments. In this work, a unique phenomenon of genetic redundancy is described in the control of Mlsc expression: Mlsc appears to be controlled by at least two unlinked loci, and the product of either one of these loci is sufficient to evoke Mlsc-specific T cell response and to act as a ligand in the deletion of self Mlsc-reactive V beta 3+ T cells. Based on these findings, we propose a possible explanation for the fact that Mls-like genes or gene products have not been identified in other species such as man.

show abstract

Properties of the Mls System: A Revised Formulation of Mls Genetics and an Analysis of T‐Cell recognition of Mls Determinants

Cited by 19 publications

References 50 publications

Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice.

Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice.

Regulatory T cells in the establishment and maintenance of self-tolerance: role of the thymic epithelium

Analysis of Mlsc genetics. A novel instance of genetic redundancy.

Contact Info

Product

Resources

About