Properties of supramolecular nanoassociates formed in aqueous solutions of biologically active compounds in low or ultra-low concentrations

Рыжкина, И. С.; Муртазина, Л. И.; Kiseleva, Yu. V.; Коновалов, А. И.

doi:10.1134/s0012501609100029

Cited by 53 publications

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“…In recent years, an idea is being actively developed that one of the mechanisms of the effect of biologically active substances at ultralow doses on biological mem branes is the formation of supramolecular nanoassoci ates of biologically active substances [3,11,12], which are adjoined by oriented water layers with special properties (density, conductivity, and charge) [13,14]. In particular, it was found that the formation of nanoassociates in dilute aqueous solutions of α toco pherol is responsible for the expression of its biological effects (changes in microviscosity of lipids and param eters of their ordering in plasma membrane) at ultralow doses [3].…”

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confidence: 99%

Dose dependences of lipid microviscosity of biological membranes induced by synthetic antioxidant potassium phenosan salt

Palmina

Chasovskaya

Belov

et al. 2012

Dokl Biochem Biophys

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Earlier, we demonstrated for the first time that the natural antioxidant α tocopherol in a wide concentra tion range can modify the structure of different lipid regions in the endoplasmic reticulum and plasma membranes of hepatocytes in experiments in vitro [1,2]. Despite the biochemical and functional differences between endoplasmic reticulum and plasma mem branes, they are characterized by similar polymodal non linear dose dependences of the effects of α toco pherol on the rigidity of superficial (~8 Å) and micro viscosity of buried (~20 Å) lipid bilayer regions. Such patterns are typical for biologically active substances, showing an effect over a wide concentration range, including ultralow doses [1,2]. The polymodality of dose dependences is associated with the statistically significant effects of α tocopherol in three concentra tion ranges. It is assumed that three possible mecha nisms of action may underlie the effect of this com pound depending on its concentration: insertion into the membrane, interaction with specific binding sites, and the information transfer through the layers of water with special physical and chemical properties [1][2][3]. It is known that the main physicochemical characteristic of antioxidants is their ability to inhibit lipid peroxidation (LPO) both in vitro and in vivo, the regulatory system of which includes the microviscosity of lipids [4].In view of above, a question arises as to what are the common and distinctive features of the effects of nat ural and synthetic agents at ultralow doses and whether the relationship between the ability of antiox idants to inhibit LPO and modify the structure of bio logical membranes is retained when they are used at ultralow doses. To answer this question, we assessed the effect of potassium salt of β (4 hydroxy 3,5 diter tbutylphenyl) propionic acid (potassium phenosan), a synthetic antioxidant, in a wide concentration range on the lipid microviscosity of EPR and PM of hepato cytes.The study was performed with 100 F 1 mice (C 57 BL × DBA 2 ). Potassium phenosan solutions were prepared by successive tenfold dilutions of the initial solution (10 -3 M) with distilled water followed by shaking on a shaker for 1 min. Plasma membrane and endoplasmic reticulum were isolated from mouse hepatocytes by centrifugation by the method of Loten [5]. Mice were sacrificed by decapitation. Protein concentration was determined by the method of Lowry [6]. The parameters characterizing the struc ture of membranes were calculated from the spectra obtained at 293 K on a Bruker 200D EPR spectrome ter (Germany) by the spin probe method [7]. The microviscosity of buried lipid bilayer regions (~20 Å) was estimated using 16 doxylstearic acid (С 16 ). The membrane microviscosity near the С 16 probe was eval uated on the basis of the rotational correlation time of the probe τ С1 and τ С2 , which was determined by the formulas for rapid anisotropic rotation of radicals [7]. The absolute values of parameters τ С1 and τ С2 in the control and after the treatment with potass...

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confidence: 99%

Dose dependences of lipid microviscosity of biological membranes induced by synthetic antioxidant potassium phenosan salt

Palmina

Chasovskaya

Belov

et al. 2012

Dokl Biochem Biophys

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“…22 As for nanoparticles with a size of an order of hundreds of nanometers, it is most probable that they are similar in nature to supramolecular structures (giant clusters) de scribed recently 23,24 and to nanoassociates, which have been found for the first time in solutions of low concentra tions. [25][26][27] The authors of these works assume that or dered structures of water along with molecules or ions of the solute participate in the formation of such associates. The plot of the nanoassociate sizes vs CTAB concentra tion in the interval below 10 -5 mol L -1 is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…It is established that the rearrangement of nanoassociates is responsible for the nonlinear change in some physicochemical properties of solutions in the region of low and superlow concentra tions, primarily for the appearance of the nonlinear con centration dependences of the specific electroconductivi ty of solutions. 25, 26 To reveal the role that can be played by CTAB nanoassociates in the formation of mixed 4-CTAB nanoparticles, we studied the dependences σ = f(C CTAB ) and D = f(C CTAB ) down to С CTAB = 10 -16 mol L -1 . We managed to detect reliably the sizes of CTAB particles to the value C CTAB = 10 -12 mol L -1 .…”

Section: Resultsmentioning

confidence: 99%

Nanosized mixed aggregates of alkylated p-sulfonatocalix[n]arenes and cetyltrimethylammonium bromide: self-organization and catalytic activity

et al. 2010

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Self organization and catalytic activity of supramolecular systems based on a series of O alkylated p sulfonatocalix[n]arenes (SCA: n = 4, 6, 8; R = Bu, Oct, Dod (Oct is octyl, Dod is dodecyl)) and cetyltrimethylammonium bromide (CTAB) were studied by dynamic light scattering, tensimetry, and spectrophotometry. In aqueous solutions containing SCA (10 -6 -10 -4 mol L -1 ) and CTAB (10 -2 -10 -12 mol L -1 ), mixed associates and SCA-CTAB micelles are formed in a wide concentration range. Their sizes (100-300 nm), properties, and reactivity depend mainly on the structure and concentration of the starting components, as well as on the nature of their associates in solutions. A relationship between the nonlinear concen tration dependences of the sizes of SCA-CTAB micelles (SCA: n = 4, 6, 8; R = Dod) and their catalytic activity in the hydrolysis of О ethyl О (4 nitrophenyl) chloromethylphosphonate was established. The study of the physiological effect on plant cells in solutions of SCA (n = 6; R = Dod), CTAB, and their mixtures showed that SCA and CTAB exerted opposite effects on the energy exchange in the wheat root cells, while a mixed solution of these substances (1 : 1) has almost no effect on the physiological state of the roots, which is due to the formation of stable CTAB-SCA aggregates that protect the biosystem from the action of the starting components.

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“…Independently, work carried out in the laboratory of Alexander Konovalov at Russian Academy of Sciences, Kazan Tatarstan, shows that highly serially diluted solutions, regardless of whether they involve organic salts, amphiphilic or hydrophobic compounds, spontaneously form clusters 100 to 300 nm in size with a surface potential −2 to −20 mV (reviewed in [27]). The team characterized the nanostructures in solution in terms of conductivity, size, surface tension and surface potential at a wide range of concentrations, but not direct imaging of the nanostructures.…”

Section: Understanding Structured Watermentioning

confidence: 99%

Illuminating Water and Life

Ho¹

2014

Entropy

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This paper reviews the quantum electrodynamics theory of water put forward by Del Giudice and colleagues and how it may provide a useful foundation for a new science of water for life. The interaction of light with liquid water generates quantum coherent domains in which the water molecules oscillate between the ground state and an excited state close to the ionizing potential of water. This produces a plasma of almost free electrons favouring redox reactions, the basis of energy metabolism in living organisms. Coherent domains stabilized by surfaces, such as membranes and macromolecules, provide the excited interfacial water that enables photosynthesis to take place, on which most of life on Earth depends. Excited water is the source of superconducting protons for rapid intercommunication within the body that may be associated with the acupuncture meridians. Coherent domains can also trap electromagnetic frequencies from the environment to orchestrate and activate specific biochemical reactions through resonance, a mechanism for the most precise regulation of gene function.

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Properties of supramolecular nanoassociates formed in aqueous solutions of biologically active compounds in low or ultra-low concentrations

Cited by 53 publications

References 1 publication

Dose dependences of lipid microviscosity of biological membranes induced by synthetic antioxidant potassium phenosan salt

Dose dependences of lipid microviscosity of biological membranes induced by synthetic antioxidant potassium phenosan salt

Nanosized mixed aggregates of alkylated p-sulfonatocalix[n]arenes and cetyltrimethylammonium bromide: self-organization and catalytic activity

Illuminating Water and Life

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