Properties of sodium-driven bacterial flagellar motor: A two-state model approach

Abstract

Bacterial flagellar motor (BFM) is one of the ion-driven molecular machines, which drives the rotation of flagellar filaments and enable bacteria to swim in viscous solutions. Understanding its mechanism is one challenge in biophysics. Based on previous models and inspired by the idea used in description of motor proteins, in this study one two-state model is provided. Meanwhile, according to corresponding experimental data, mathematical relationship between BFM membrane voltage and pH value of the environment, and relationship between internal and external sodium concentrations are given. Therefore, with model parameter values obtained by fitting theoretical results of torque-speed relation to recent experimental data, many biophysical properties of bacterial flagellar motor can be obtained for any pH values and any external sodium concentrations. Including the rotation speed, stall torque (i.e. the torque generated by BFM), rotation dispersion, and rotation randomness. In this study, the single-stator BFM will be firstly analyzed, and then properties of multiple-stator BFM are addressed briefly. Author SummaryBased on previous models for BFM and the idea used in the modeling of motor protein kinesin, one two-state model is present to discuss biophysical properties of BFM. From this model torque-speed relationship, dispersion and randomness of single-stator BFM can be obtained for any pH values and any external sodium concentrations. Meanwhile properties of BFM with multiple stators are also discussed.1