Properties of Silver Nanoparticles Prepared by the Electric Spark Dispersion Method

Журавков, С. П.; Plotnikov, Evgenii; Martemiyanov, Dmitry; Yavorovsky, N.; Hasse, Ulrich; Zander, Stefan

doi:10.4028/www.scientific.net/amr.872.74

Cited by 9 publications

(12 citation statements)

References 4 publications

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“…The advancement of new methods of AgNPs preparation serves to develop nanoparticles with higher biomedical activity, higher stability, and lower toxicity [ 16 , 17 , 18 , 19 ]. Usually, various organic solvents and reducing agents are used for AgNPs production, and as a result, their traces could remain in the final AgNPs.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Cancer Cells Susceptibility to Silver Nanoparticles Produced by Electron Beam

et al. 2023

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Introduction: Silver nanoparticles (AgNPs) have a wide range of bioactivity, which is highly dependent on particle size, shape, stabilizer, and production method. Here, we present the results of studies of AgNPs cytotoxic properties obtained by irradiation treatment of silver nitrate solution and various stabilizers by accelerating electron beam in a liquid medium. Methods: The results of studies of morphological characteristics of silver nanoparticles were obtained by transmission electron microscopy, UV-vis spectroscopy, and dynamic light scattering measurements. MTT test, alamar blue test, flow cytometry, and fluorescence microscopy were used to study the anti-cancer properties. As biological objects for standard tests, adhesive and suspension cell cultures of normal and tumor origin, including prostate cancer, ovarian cancer, breast cancer, colon cancer, neuroblastoma, and leukemia, were studied. Results: The results showed that the silver nanoparticles obtained by irradiation with polyvinylpyrrolidone and collagen hydrolysate are stable in solutions. Samples with different stabilizers were characterized by a wide average size distribution from 2 to 50 nm and low zeta potential from −7.3 to +12.4 mV. All AgNPs formulations showed a dose-dependent cytotoxic effect on tumor cells. It has been established that the particles obtained with the combination of polyvinylpyrrolidone/collagen hydrolysate have a relatively more pronounced cytotoxic effect in comparison to samples stabilized with only collagen or only polyvinylpyrrolidone. The minimum inhibitory concentrations for nanoparticles were less than 1 μg/mL for various types of tumor cells. It was found that neuroblastoma (SH-SY5Y) is the most susceptible, and ovarian cancer (SKOV-3) is the most resistant to the action of silver nanoparticles. The activity of the AgNPs formulation prepared with a mixture of PVP and PH studied in this work was higher that activity of other AgNPs formulations reported in the literature by about 50 times. Conclusions: The results indicate that the AgNPs formulations synthesized with an electron beam and stabilized with polyvinylpyrrolidone and protein hydrolysate deserve deep study for their further use in selective cancer treatment without harming healthy cells in the patient organism.

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Section: Introductionmentioning

confidence: 99%

A Comparative Study of Cancer Cells Susceptibility to Silver Nanoparticles Produced by Electron Beam

et al. 2023

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“…The resulting nanostructured product can be used in designing a new filter system for water purification. Detail process description and confirmation nanoscale size of the materials prepared by this method, are reflected in works [9][10][11] . The aim of this work was to crate and study the physicochemical and sorption properties of new modified sorbents with nanostructured surface based on aluminum and iron, obtained by the electro-dispersion method.…”

Section: Introductionmentioning

confidence: 99%

Water Purification with Natural Sorbents: Effect of Surface Modification with Nano-structured Particles

et al. 2015

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Modified nanostructured sorbents are widely used in water treatment processes. In this work, aluminum and iron particles prepared by electro-spark dispersion have been applied as modifiers. Zeolite samples from "Holinskoe" mineral deposit (Russia, Republic of Buryatia), with a size smaller than 0.1 mm, have been modified with aluminum and iron particles, using a sol-gel process. The properties of the modified materials have been determined by means of sorption test, when removing ions Pb{2+}, Fe{3+} and Cd{2+} from the model solutions in static conditions. Using the method of thermal desorption of nitrogen (BET) it has been shown that increasing the iron content in the samples of nanostructured modified sorbents does not affect the increase in specific surface area and pore volume of the samples. Ions concentrations have been analyzed by stripping voltammetry and photocolorimetry. As a result, modified sorbents revealed a high efficiency sorption of heavy metals

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“…This device was developed and produced in Tomsk Polytechnic University (Tomsk, Russia). The nanoparticles preparation procedure is described at length in our previous work (Zhuravkov et al, 2014;. In brief, the reactor was filled with gold or silver granules of different forms and diameters.…”

Section: Nanoparticles Preparationmentioning

confidence: 99%

“…Even narrow differences in nanoparticle sizes may cause significantly different overall biological response (Coradeghini et al, 2013). Here we applied a comet assay for comparative investigation of genotoxicity of gold (Au) and silver (Ag) nanoparticles, prepared by the modified electric spark dispersion method in water.Despite of plenty different method of nanoparticles production, including even laser ablation (Stašić, et al, 2016), modified electric spark dispersion allowed to produce nanoparticles of different shapes and properties by changing experimental medium and parameters of electric impulses (Zhuravkov et al, 2014). The comet assay is a convenient and informative method for genotoxicity studies (Vandghanooni et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Genotoxicity of Silver and Gold Nanoparticles Prepared by the Electric Spark Dispersion Method

2017

J App Pharm Sci

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Objective: There is plenty of information dedicated to nanomaterial toxicity, but it is often contradictory. This work presents results of the comparative investigation of silver and gold nanoparticles genotoxicity and genotoxic effect of water medium after the spark dispersion process. Material and methods:The nanoparticles were produced in water by the modified energy-efficient electric spark dispersion method. The comet assay, transmission electron microscopy, DLS spectroscopy and thermal desorption of nitrogen (BET method) were used for the investigation. Results:The silver nanoparticles showed a genotoxic effect which appeared at concentration of 0.03 mg/ml and above. The application of gold nanoparticles did not lead to a significant DNA damage at concentrations range of 0.01 -0.03 mg/ml. However, a notable level of genotoxicity was observed at concentrations of gold nanoparticles about 0.1 mg/ml. Supernatant water medium (with trace of Au and Ag metals) following the spark dispersion process showed no genotoxic action. Conclusion: Accordingly, the testing of the nanoparticles produced by the electric spark dispersion in water revealed more expressed genotoxic effect of silver nanoparticles in comparison with the gold ones.

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Properties of Silver Nanoparticles Prepared by the Electric Spark Dispersion Method

Cited by 9 publications

References 4 publications

A Comparative Study of Cancer Cells Susceptibility to Silver Nanoparticles Produced by Electron Beam

A Comparative Study of Cancer Cells Susceptibility to Silver Nanoparticles Produced by Electron Beam

Water Purification with Natural Sorbents: Effect of Surface Modification with Nano-structured Particles

Comparative Study of Genotoxicity of Silver and Gold Nanoparticles Prepared by the Electric Spark Dispersion Method

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