Properties of ionic liquids on Au surfaces: non-conventional anion exchange reactions with carbonate

Ratel, Mathieu; Branca, Mathieu; Breault-Turcot, Julien; Zhao, Sandy Shuo; Chaurand, Pierre; Schmitzer, Andreea R.; Masson, Jean-François

doi:10.1039/c1cc13914b

Cited by 12 publications

(10 citation statements)

References 23 publications

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“…MS analysis provides molecular fingerprinting of the materials non-specifically adsorbed on various surface chemistries. Protein adsorption to surfaces is well-documented for serum-based analysis. − Because non-specific adsorption from cell lysate has never been analyzed by MS, the interaction between the peptide SAMs and cell lysate must be investigated. Non-specifically adsorbed proteins were analyzed after SAM trypsin digestion of the proteins.…”

Section: Resultsmentioning

confidence: 99%

Non-specific Adsorption of Crude Cell Lysate on Surface Plasmon Resonance Sensors

Aubé¹,

Breault-Turcot²,

Chaurand³

et al. 2013

Langmuir

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Non-specific adsorption of the molecular components of biofluids is ubiquitous in the area of biosensing technologies, severely limiting the use of biosensors in real-world applications. The surface chemistries developed to prevent non-specific adsorption of crude serum are not necessarily suited for sensing in other biosamples. In particular, the diagnostic potential of differential expression of proteins in tissues makes cell lysate attractive for disease diagnostics using solid biopsies. However, crude cell lysate poses a significant challenge for surface chemistries because of a large concentration of highly adherent lipids. Contrary to the non-specific adsorption in crude serum being suppressed by hydrophilic surfaces, the surface plasmon resonance (SPR) analysis of serine-, aspartic-acid-, histidine-, leucine-, and phenylalanine-based peptide monolayers revealed that hydrophobic and positively charged peptides decreased non-specific adsorption when using lysate from HEK 293FT cells. A polyethylene glycol (PEG) monolayer resulted in 2-fold greater fouling than the best peptide [3-MPA-(His)2(Leu)2(Phe)2-OH] under the same conditions. Matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analysis of the adsorbate from cell lysate confirmed that lipids are the main source of non-specific adsorption. Importantly, the mass spectrometry (MS) study revealed that both the number of lipids identified and their intensity decreased with decreasing non-specific adsorption. A peptide monolayer thus provides an efficient mean to suppress non-specific adsorption from this human cell lysate.

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Section: Resultsmentioning

confidence: 99%

Non-specific Adsorption of Crude Cell Lysate on Surface Plasmon Resonance Sensors

Aubé¹,

Breault-Turcot²,

Chaurand³

et al. 2013

Langmuir

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“…Matrix deposition was performed by successive evaporation of 0.5 lL of matrix solution on the digested spots to analyze proteins and beside the digested spots for lipid analysis. MS analysis of the adsorbed material was performed with a MALDIÀTOF/TOF mass spectrometer (Bruker Daltonics, Bremen, Germany) as previously described (Ratel et al, 2011).…”

Section: Maldi-tof Analysis Of Adsorbed Proteinsmentioning

confidence: 99%

Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi

Singh

Nand

Sarita

et al. 2019

Arabian Journal of Chemistry

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Comparative study for non-specific adsorption of cell lysate and serum on recently developed biosensor surfaces is reported. Different surface chemistries including, polyethylene glycol (PEG), a-cyclodextrin (CD), hydrogel dextran and surface initiated polymerization (SIP) based gold surfaces were taken into account for this study. Various techniques including, surface plasmon resonance imaging (SPRi) and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDIÀTOF/TOF MS) technique to evaluate the surfaces with Fourier transform infrared spectroscopy (FTIR) for the confirmation of surface fabrication were used. A high non-specific adsorption response of cell lysate and serum was observed on these so-called non-fouling surfaces. The obtained results from this comparative study provided some hope to explore SIP and dextran surface as a universal platform for biosensor applications. SIP produced best result and showed high sensitivity and minimum non-specific adsorption. We believe that this

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“…182 Ionic liquid SAM undergoes unexpected anion exchange with carbonate when immobilized to SPR sensors. 183 They can be synthesized with a carboxylic acid group, which would be available for immobilizing a bioreceptor. 183 Ionic liquids are zwitterionic and thus may provide good resistance to nonspecific adsorption.…”

Section: Stability Of Molecular Receptorsmentioning

confidence: 99%

“…183 They can be synthesized with a carboxylic acid group, which would be available for immobilizing a bioreceptor. 183 Ionic liquids are zwitterionic and thus may provide good resistance to nonspecific adsorption. Thus, they should be investigated more thoroughly for biosensing.…”

Section: Stability Of Molecular Receptorsmentioning

confidence: 99%

Modern surface plasmon resonance for bioanalytics and biophysics

Couture

Zhao

Masson

2013

Phys. Chem. Chem. Phys.

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160

139

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Physical chemistry, materials science, analytical chemistry and engineering greatly contributed to the increasing popularity of bioanalytical and biophysical applications of surface plasmon resonance (SPR) by providing novel materials, surface chemistry, instrumental concepts, and theory to further understand the plasmonic phenomenon and support innovation in SPR. This perspective article portrays the contemporary state of SPR-based techniques and establishes a list of challenges to be overcome for improving bioanalytical and biophysical applications of plasmonics and surface plasmon resonance.

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Properties of ionic liquids on Au surfaces: non-conventional anion exchange reactions with carbonate

Cited by 12 publications

References 23 publications

Non-specific Adsorption of Crude Cell Lysate on Surface Plasmon Resonance Sensors

Non-specific Adsorption of Crude Cell Lysate on Surface Plasmon Resonance Sensors

Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi

Modern surface plasmon resonance for bioanalytics and biophysics

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