Properties of hybrid ribosomes formed from the subunits of mesophilic and thermophilic bacteria

Altenburg, Lewis C.; Saunders, Grady F.

doi:10.1016/0022-2836(71)90332-9

Cited by 20 publications

(3 citation statements)

References 29 publications

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“…Nevertheless, functional homology has been retained at least among diverse members of the order Eubacteriales. This is evident in the observation (43,298) that 50S ribosomal subunits from the genus Bacillus, including the thermophile B. stearothermophilus (2), can combine with E. coli 30S subunits to form hybrid ribosomes which are active in protein synthesis. Furthermore, Nomura and his colleagues (211) have reconstituted functional, hybrid 30S ribosomal subunits from the component proteins and RNA from different eubacteriales, including E. coli, Micrococcus lysodeikticus, Azotobacter vinelandii, and B. stearothermophilus.…”

Section: General Features Of Prokaryotic Ribosomes Diversity In Bactementioning

confidence: 99%

Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.

Pace¹

1973

Bacteriological Reviews

107

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Section: General Features Of Prokaryotic Ribosomes Diversity In Bactementioning

confidence: 99%

Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.

Pace¹

1973

Bacteriological Reviews

107

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“…This is the expected result if the heat stability of ribosomes is due to their overall structure and not the r-RNA alone. This is supported by the work of ALTENBERG and SAUNDERS (1971) who demonstrated that the ribosomal subunit from a thermophilic bacterium can influence the structure of the subunit from a mesophilic bacterium and increase the heat stability of the functional unit.…”

Section: Evolution Of the Ribosomal Cistronsmentioning

confidence: 69%

Current Topics in Microbiology and Immunology

1974

Current Topics in Microbiology and Immunology

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Published by Springer-Verlag Price : 129.95 € Hardcover Pages : 255, Vol 340, 2010 ISBN : 978-3-642-03857-0 DOI : 10.1007 Reviewed by Rajendra Kumar and Rupali Sharma "Current topics in microbiology and immunology: Immunological Synapse" Reviewed by Rajendra Kumar and Rupali Sharma.Published by Springer pp 255, Price 129.95 €(Hardcover).The science of immunology has seen outsized transformation from a medicine named vaccination to a contemporary applied science focused on versatile development in molecular medicine. Fundamental principles of immunology along with their clinical applications provide an exciting platform for specialization. Immune system is able to engender variety of cells and molecules capable of recognizing and removing infinite number of foreign intruders. Functioning of immune system is dependent on a reliable cell-cell communication, which forms the basis of phenomena of Immunological Synapse.Current volume is an attempt of an additional step toward understanding the immunological synapse. It has the essence of most consensual volume, as editors invited a number of key researchers in the field, to provide perspectives and current understanding of the molecular process in the wake of the formation and function of the immunological synapse. This book targets the professionals as well as the medical or bioscience students by emphasizing on the recent advancement in immunological synapse.In first chapter, Michael L. Dustin has enlighten the function of immunological synapse and have explained the basic features of molecular interactions in the synapse using the lipid bi-layer on glass surface model. Further he has also explained that how this model along with fluorescent microscopy, can be exploited for the measurement of 2-D affinity and kinetics in cell-cell contact.Junsang Doh and Matthew F. Krummel have contributed the second chapter. They have reviewed the pattern of cell-cell communication in T lymphocytes and antigen presenting cells and extrapolated it into the framework of immune system as a whole. Here, the authors have suggested various patterns of cell-cell communication in the immune system and have described that how each type of pattern suffice the need of an efficient communication in the system. Yolanda R. Carrasco has presented his perspective on B-cell synapse by spreading-contraction response theory of B cell activation in the succeeding chapter. Here, authors have suggested that immunological synapse is crucial for increasing the sensibility of B cells to antigen. The authors have discussed the role of single cell imaging techniques like real-time confocal microscopy and total interference reflection fluorescence microscopy in the study of the molecular mechanisms beneath B cell activation.Since it is crucial to control the immune response for maintaining the self-tolerance, the inhibitory immune synapse comes into the picture. This view has been presented by Philipp Eissmann and Daniel M. Davis. Here, the authors have discussed the role of supra-molecular assemblies to co...

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“…In vitro protein synthetic systems from B. stearothermophilus have been compared with systems from E. coli and mesophilic bacilli with regard to the influences of temperature, ionic characteristics, artificial messenger ribonucleic acid (RNA) composition, and source of transfer RNA on the incorporation of amino acids into polypeptides (13,36). Efforts have also been made to determine the relative contributions to heat stability by individual ribosome components: ribosomal subunits, RNA, and proteins (1,14,25).…”

mentioning

confidence: 99%

Ribosomes, Polyribosomes, and Deoxyribonucleic Acid from Thermophilic, Mesophilic, and Psychrophilic Clostridia

1973

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Analysis of deoxyribonucleic acid (DNA) from four species of Clostridium, including two thermophiles, a mesophile, and a psychrophile, revealed no obvious relationship between growth temperature and DNA base composition. The melting temperatures (Tm) of the DNA from the four species varied no more among the thermophilic, mesophilic, and psychrophilic species than among many related mesophilic species. Characterization of ribosomes from the clostridia by means of optical rotatory dispersion yielded similar spectra in common with other unrelated organisms. Only small differences were noted in the base composition of ribosomal ribonucleic acid (RNA) and in the amino acid composition of ribosomal proteins, including half-cystine content, as determined by cysteic acid analysis, and accessible sulfhydryl groups, as determined by titration with dithiobis (2-nitrobenzoic acid). Except for the two thermophiles, the ribosomal protein electrophoretic patterns were dissimilar. No unusual thermal stability was manifested in the Tm values of thermophile ribosomal RNA. However, thermophile ribosome Tm values (69 C) were higher than were mesophile and psychrophile Tm values (64 C). Ribosomes from the four clostridial species were also examined in regard to the effect of heat on their functional integrity, measured by their activity in poly U-directed '4C-phenylaline incorporation, and their gross physical integrity, measured by sucrose gradient analysis. The Td s values (temperature which produces 50% inactivation after 5 min) was found to be 70 and 72 C for the two thermophiles C. tartarivorum and C. thermosaccharolyticum, respectively; 57 C for a mesophile, C. pasteurianum; and 53 C for a psychrophile, Clostridium sp. strain 69. At 55 C, little effect was seen on the thermophile ribosomes, but the mesophile ribosomes lost 90% of their activity in 1 hr, and psychrophile ribosomes lost 100% of their activity within 10 min. According to sucrose gradient profiles, heating at 55 C results in dissociation of mesophile ribosomes and aggregation of psychrophile ribosomes. Thermophile S-100 fractions were also more thermostable than were mesophile or psychrophile S-100 fractions. The Td, I values were 69 C for C. tartarivorum and C. thermosaccharolyticum S-100 and 41 C for C. pasteurianum and Clostridium sp. strain 69 S-100. The effect of heat on the endogenous incorporation of '4C-valine by polysomes was also examined. In the case of thermophile polysomes, the extent of incorporation at 55 and 37 C was about equal. In the case of mesophile and psychrophile polysomes, the extent at 55 C was 44 and 39%, respectively, of the value at 37 C. The initial rates of incorporation in all four cases were greater at 55 C than at 37 C. Accumulating evidence for unique from the obligate thermophile, Bacillus stearothermostability of macromolecules from ther-thermophilus, fall within the range characterismophilic organisms has thus far not implicated tic of Bacillus values in general (9). Studies with deoxyribonucleic acid (DNA) insofar as melting A...

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Properties of hybrid ribosomes formed from the subunits of mesophilic and thermophilic bacteria

Cited by 20 publications

References 29 publications

Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.

Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.

Current Topics in Microbiology and Immunology

Ribosomes, Polyribosomes, and Deoxyribonucleic Acid from Thermophilic, Mesophilic, and Psychrophilic Clostridia

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