Properties of human blood monocytes. II. Monocytes from healthy adults are highly heterogeneous within and among individuals

Hudig, Dorothy; Hunter, Kenneth W.; Diamond, William J.; Redelman, Doug

doi:10.1002/cyto.b.21141

Cited by 18 publications

(15 citation statements)

References 118 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In that field, we found that HLA-DR expression on monocytes (considered as an activation marker) did not vary significantly with age, in accordance with a previous study. 22 In contrast, the expression of CD64 and CD163 slightly increased with age on the three monocyte subsets studied. The increase in CD64 on monocytes was previously associated with their increased phagocytosis capacity and inversely correlated with their TNFa secretion in various inflammatory pathologies.…”

Section: Discussionmentioning

confidence: 85%

Distinct effect of age, sex, and CMV seropositivity on dendritic cells and monocytes in human blood

et al. 2017

View full text Add to dashboard Cite

We analyzed the impact of age, sex, and CMV on blood monocyte and dendritic cell (DC) subpopulations in 256 healthy individuals aged from 19 to 96 years. Flow cytometry was performed on whole blood within the 4 h following blood drawing. Myeloid (mDC) and plasmacytoid DC (pDC), classical, intermediate, and nonclassical monocytes were enumerated by means of TruCount tubes (BD Biosciences). We provided reference values for mDC, pDC and the three monocyte subpopulations. The numbers of classical, intermediate, and nonclassical monocytes slightly increased with age while the numbers of mDC and pDC did not vary significantly. The level of expression of CD64 and CD163 on monocytes significantly increased with age while HLA-DR expression did not vary significantly. More precisely, CD163 expression level on intermediate monocyte slightly increased with age in women only (Spearman P = 0.019) while CD64 expression increased on monocytes in CMV-positive individuals only. We observed that sex had almost no impact on the numbers of monocytes and DC and on their expression level of CD64 and HLA-DR. We observed a significant decrease in the numbers of pDC with age in CMV-positive individuals, but not in CMV negative individuals. This suggests that the lifelong subclinical infection by CMV could influence the number of circulating DC of lymphoid origin. In contrast, CMV serostatus had no significant impact on absolute numbers of mDC and monocytes.

show abstract

Section: Discussionmentioning

confidence: 85%

Distinct effect of age, sex, and CMV seropositivity on dendritic cells and monocytes in human blood

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Classical monocytes are phagocytic cells and preferentially express genes involved in angiogenesis, wound healing, and coagulation, intermediate monocytes are pro-inflammatory and with the capability of antigen presentation and T cell activation, while non-classical monocytes display patrolling properties. These subsets expressed high levels of HLA-DR and CD33 and different levels of CD64 ( 39 , 40 ) (Figure 8 A). Very few studies are available on the possible interaction between MSC and monocytes ( 41 , 42 ).…”

Section: Resultsmentioning

confidence: 99%

Long-Term Clinical and Immunological Profile of Kidney Transplant Patients Given Mesenchymal Stromal Cell Immunotherapy

et al. 2018

View full text Add to dashboard Cite

We report here the long-term clinical and immunological results of four living-donor kidney transplant patients given autologous bone marrow-derived mesenchymal stromal cells (MSCs) as part of a phase 1 study focused on the safety and feasibility of this cell therapy. According to study protocols implemented over time, based on initial early safety findings, the patients were given MSC at day 7 posttransplant (n = 2) or at day −1 pretransplant (n = 2) and received induction therapy with basiliximab and low-dose rabbit anti-thymocyte globulin (RATG) or RATG alone, and were maintained on low-dose ciclosporin (CsA)/mycophenolate mofetil (MMF). All MSC-treated patients had stable graft function during the 5- to 7-year follow-up, without increased susceptibility to infections or neoplasm. In three MSC recipients, but not historical control patients, circulating memory CD8+ T cell percentages remained lower than basal, coupled with persistent reduction of ex vivo donor-specific cytotoxicity. Two patients showed a long-lasting increase in the regulatory T cell/memory CD8+ T cell ratio, paralleled by high circulating levels of naïve and transitional B cells. In one of these two patients, CsA was successfully discontinued, and currently the low-dose MMF monotherapy is on the tapering phase. The study shows that MSC therapy is safe in the long term and could promote a pro-tolerogenic environment in selected patients. Extensive immunomonitoring of MSC-treated kidney transplant recipients could help selection of patients for safe withdrawal of maintenance immunosuppressive drugs (NCT00752479 and NCT02012153).

show abstract

“…Several biomarkers have been associated with M1/M2 profiles. CD163, the high-affinity scavenger receptor for the haemoglobin-haptoglobin complex, is selectively expressed on M2 macrophages and monocytes [ 6 ], whereas CD80 (B7-1), a costimulatory signal for T cell activation and survival, is preferentially expressed on M1 macrophages. The different functions of M1 and M2 macrophages have been demonstrated in various tissues that are relevant for ARDs [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Age-related M1/M2 phenotype changes in circulating monocytes from healthy/unhealthy individuals

Costantini

Viola

Berretta

et al. 2018

Aging

View full text Add to dashboard Cite

Macrophage polarization is a candidate biomarker of disease-related inflammatory status, but its modulation during aging has not been investigated. To do this, the M1/M2 profile was assessed by CD80/CD163 gating in classical (CD14++CD16-), intermediate (CD14++CD16+), and non-classical (CD14lowCD16+) monocytes from 31 healthy subjects (CTRs) of different ages. Cytofluorimetric analysis showed a significantly different CD80/CD163 distribution in the three subsets, as more than 80% of classical and intermediate monocytes were CD80+CD163+, whereas most non-classical monocytes were CD80-CD163- and CD163+. Non-classical CD163+ monocytes were significantly higher whereas classical CD163+ and CD80-CD163- monocytes significantly lower in older than younger CTRs (cut-off, 65 years), suggesting different age-related trends for M2 subsets. To establish whether an M1/M2 imbalance could be associated with disease, 21 patients with acute myocardial infarction (AMI) were compared with older CTRs. The AMI patients showed a significantly decreased proportion of CD163+CD80+ and an increased proportion of CD163+ and CD163-CD80- cells among classical monocytes, opposite trends to those observed in healthy aging. Moreover, a significantly greater proportion of intermediate and non-classical CD80+ monocytes suggested a shift to a pro-inflammatory phenotype. Overall, CD163/CD80 cytofluorimetric characterization of circulating monocytes provides additional information about their polarization and could be an innovative tool to monitor aging.

show abstract

Properties of human blood monocytes. II. Monocytes from healthy adults are highly heterogeneous within and among individuals

Cited by 18 publications

References 118 publications

Distinct effect of age, sex, and CMV seropositivity on dendritic cells and monocytes in human blood

Distinct effect of age, sex, and CMV seropositivity on dendritic cells and monocytes in human blood

Long-Term Clinical and Immunological Profile of Kidney Transplant Patients Given Mesenchymal Stromal Cell Immunotherapy

Age-related M1/M2 phenotype changes in circulating monocytes from healthy/unhealthy individuals

Contact Info

Product

Resources

About