Properties of cowpea chlorotic mottle virus, its protein and nucleic acid

Bancroft, J.B.; Hiebert, E.; Rees, M. W.; Markham, Roy

doi:10.1016/0042-6822(68)90232-8

Cited by 131 publications

(66 citation statements)

References 31 publications

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“…Cowpea°chlorotic°mottle°virus,°(CCMV)°4.6°MDa° [41] is a member of the Bromoviridae family bromovirus group of plant viruses. The capsid is composed of 180 chemically identical 19.8 kDa subunits (190 amino acids each) arranged with a T ϭ 3 icosahedral quasisymmetry.…”

Section: Do Protein Complexes Collapse Upon Introduction To the Gas Pmentioning

confidence: 99%

Sizing large proteins and protein complexes by electrospray ionization mass spectrometry and ion mobility

Kaddis

Lomeli

Yin

et al. 2007

J. Am. Soc. Mass Spectrom.

146

149

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Mass spectrometry (MS) and ion mobility with electrospray ionization (ESI) have the capability to measure and detect large noncovalent protein-ligand and protein-protein complexes. Using an ion mobility method of gas-phase electrophoretic mobility molecular analysis (GEMMA), protein particles representing a range of sizes can be separated by their electrophoretic mobility in air. Highly charged particles produced from a protein complex solution using electrospray can be manipulated to produce singly charged ions, which can be separated and quantified by their electrophoretic mobility. Results from ESI-GEMMA analysis from our laboratory and others were compared with other experimental and theoretically determined parameters, such as molecular mass and cryoelectron microscopy and X-ray crystal structure dimensions. There is a strong correlation between the electrophoretic mobility diameter determined from GEMMA analysis and the molecular mass for protein complexes up to 12 MDa, including the 93 kDa enolase dimer, the 480 kDa ferritin 24-mer complex, the 4.6 MDa cowpea chlorotic mottle virus (CCMV), and the 9 MDa MVP-vault assembly. ESI-GEMMA is used to differentiate a number of similarly sized vault complexes that are composed of different N-terminal protein tags on the MVP subunit. The average effective density of the proteins and protein complexes studied was 0.6 g/cm 3 . Moreover, there is evidence that proteins and protein complexes collapse or become more compact in the gas phase in the absence of water. (J Am Soc Mass Spectrom 2007, 18, 1206 -1216

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Section: Do Protein Complexes Collapse Upon Introduction To the Gas Pmentioning

confidence: 99%

Sizing large proteins and protein complexes by electrospray ionization mass spectrometry and ion mobility

Kaddis

Lomeli

Yin

et al. 2007

J. Am. Soc. Mass Spectrom.

146

149

View full text Add to dashboard Cite

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“…20 800; (iv) the genome consists of four single-stranded RNA molecules of which only the three largest were required for infectivity; (v) at pH 7.0, the virions sediment more slowly than at lower pH values probably because they swell; and (vi) a narrow host range, with most hosts being only locally infected. Yamazaki & Kaesberg (1963), for BBM2 from Miki & Knight (1965), for BBM3 from Wittman & Paul (1961), for CCM1 from Bancroft et al (1968), for CCM2 and CCM3 (the bean yellow stipple streak strain of CCMV) from Rees & Short (1977), for BM from Stubbs & Kaesberg (1964), for AM1 and AM2 (AMV-S and AMV-425, respectively) from their known primary sequences (Collot et aL, 1977;van Beynum et al, 1977), for the cucumoviruses cucumber mosaic (CM), peanut stunt (PS) and tomato aspermy (TA) from Kaper & Waterworth (1981) and for CYB from the work reported in this paper. The relatedness of the data sets was calculated using the squared Euclidean distance metric.…”

Section: Discussionmentioning

confidence: 99%

Cassia Yellow Blotch Virus: a New Bromovirus from an Australian Native Legume, Cassia pleurocarpa

Dale

Gibbs

Behncken

1984

Journal of General Virology

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SUMMARY Cassia yellow blotch virus (CYBV) is a previously undescribed bromovirus infectingthe Australian indigenous legume, Cassia pleurocarpa, in western Queensland.Although CYBV infected test plants in several families, symptoms were usually restricted to local lesions on the inoculated leaves. Systemic infection occurred in only eight leguminous species and in the non-legume, Nicotiana clevelandii. Chenopodium amaranticolor was a suitable host for local lesion assays and CYBV was readily purified from N. clevelandii. Purified CYBV preparations, when negatively stained, contained isometric particles 25 to 27 nm in diameter with a penetrated central core of approx. 10 nm. The virions sedimented as a single component with a sedimentation coefficient (SE0,w) of 85S and had an isoelectric point of 3.6. At pH 7.0, the virions sedimented more slowly than at pH 5-0 and 6.5 probably because they swelled. Purified virions contained a single coat protein species with a molecular weight of 20800 comprising 196 amino acid residues as estimated by FITMOL analysis of the amino acid composition. The virion genome consisted of four single-stranded RNA molecules of which only the three largest were required for infectivity. CYBV appeared to be serologically unrelated to three other bromoviruses and this lack of relationship correlated with differences in their coat protein amino acid compositions.

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“…Previous reports (Bancroft et al 1968 ;Bancroft, 197 I) suggested that the RNA in aged virus became degraded and was thus non-infectious. Breakage of the encapsidated RNA was believed to occur both in vivo and in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…Breakage of the encapsidated RNA was believed to occur both in vivo and in vitro. A mechanism of in vivo degradation of CCMV-RNA has never been established, but the most frequent suggestions include enzymatic reactions (Bancroft et al 1968;Dawson & Kuhn, I974), oxidative reactions (Bancroft,I97I), and direct or indirect temperature effects (Dawson & Kuhn,I974). The objectives of this study were to evaluate critically the four RNA species associated with CCMV preparations with different specific infectivities, to attempt to recover highly infectious RNA from CCMV with low infectivity, and to improve the method for recovering total RNA from CCMV.…”

Section: Introductionmentioning

confidence: 99%

Highly Infectious RNA Isolated from Cowpea Chlorotic Mottle Virus with Low Specific Infectivity

Wyatt

Kuhn

1977

Journal of General Virology

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SUMMARYRecovery and specific infectivity of infectious RNA from cowpea chlorotic mottle virus of low specific infectivity (14 to 2I day infections) were greatly improved by using antioxidants during virus purification and RNA extraction, and by disrupting coat protein with pronase before phenol-SDS extraction. Total infectivity of RNA from virus of low infectivity was increased over 30 times. RNA profiles obtained using polyacrylamide gels were then similar for virus with high (4 to 7 day infections) or low specific infectivity. Low specific infectivity, therefore, seems to be caused by alteration of the coat protein or of the protein-RNA interaction in intact virus particles.

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Properties of cowpea chlorotic mottle virus, its protein and nucleic acid

Cited by 131 publications

References 31 publications

Sizing large proteins and protein complexes by electrospray ionization mass spectrometry and ion mobility

Sizing large proteins and protein complexes by electrospray ionization mass spectrometry and ion mobility

Cassia Yellow Blotch Virus: a New Bromovirus from an Australian Native Legume, Cassia pleurocarpa

Highly Infectious RNA Isolated from Cowpea Chlorotic Mottle Virus with Low Specific Infectivity

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