Properties of Corneas Reconstructed with Cultured Human Corneal Endothelial Cells and Human Corneal Stroma

Amano, Shiro; Mimura, Tatsuya; Yamagami, Satoru; Osakabe, Yasuhiro; Miyata, Kazunori

doi:10.1007/s10384-005-0245-5

Cited by 21 publications

(12 citation statements)

References 18 publications

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“…Cultured corneal endothelial cells were then seeded onto recipient corneas followed by centrifugation. They were then cultured and entirely transplanted into the recipient eye and attached with sutures [104][105][106]. Functional studies of reconstructed corneas have shown that they achieve a maximum of 75% of the pump function of normal corneas [106,107].…”

Section: Corneal Endotheliummentioning

confidence: 99%

Cornea and Ocular Surface Treatment

Miguel

Alió²,

Arnalich‐Montiel³

et al. 2010

CSCR

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In addition to being a protective shield, the cornea represents two thirds of the eye's refractive power. Corneal pathology can affect one or all of the corneal layers, producing corneal opacity. Although full corneal thickness keratoplasty has been the standard procedure, the ideal strategy would be to replace only the damaged layer. Current difficulties in corneal transplantation, mainly immune rejection and shortage of organ supply, place more emphasis on the development of artificial corneas. Bioengineered corneas range from prosthetic devices that solely address the replacement of the corneal function, to tissue-engineered hydrogels that allow regeneration of the tissue. Recently, major advances in the biology of corneal stem cells have been achieved. However, the therapeutic use of these stem cell types has the disadvantage of needing an intact stem cell compartment, which is usually damaged. In addition, long ex vivo culture is needed to generate enough cell numbers for transplantation. In the near future, combination of advanced biomaterials with cells from abundant outer sources will allow advances in the field. For the former, magnetically aligned collagen is one of the most promising ones. For the latter, different cell types will be optimal: 1) for epithelial replacement: oral mucosal epithelium, ear epidermis, or bone marrow- mesenchymal stem cells, 2) for stromal regeneration: adipose-derived stem cells and 3) for endothelial replacement, the possibility of in vitro directed differentiation of adipose-derived stem cells towards endothelial cells provides an exciting new approach.

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Section: Corneal Endotheliummentioning

confidence: 99%

Cornea and Ocular Surface Treatment

Miguel

Alió²,

Arnalich‐Montiel³

et al. 2010

CSCR

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“…Although the HCECs have limited proliferative capacity in vivo , these cells have the ability to proliferate under in vitro culture conditions [ 18 , 25 , 29 , 30 , 31 , 32 , 36 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 ,…”

Section: Cell Sources For Tissue Engineering Of Corneal Endotheliumentioning

confidence: 99%

“…The feasibility of using devitalized corneas or corneas denuded of endothelium as substrate for HCECs is studied extensively [ 27 , 29 , 30 , 31 , 62 , 67 , 71 , 97 , 137 , 138 ]. They are applicable without substantial redesign as they provide the desired shape, mechanical support, and transparency.…”

Section: Substrates For Cultivation Of Human Corneal Endothelial Cmentioning

confidence: 99%

Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium

Navaratnam

Utheim

Rajasekhar

et al. 2015

JFB

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Corneal endothelium is a single layer of specialized cells that lines the posterior surface of cornea and maintains corneal hydration and corneal transparency essential for vision. Currently, transplantation is the only therapeutic option for diseases affecting the corneal endothelium. Transplantation of corneal endothelium, called endothelial keratoplasty, is widely used for corneal endothelial diseases. However, corneal transplantation is limited by global donor shortage. Therefore, there is a need to overcome the deficiency of sufficient donor corneal tissue. New approaches are being explored to engineer corneal tissues such that sufficient amount of corneal endothelium becomes available to offset the present shortage of functional cornea. Although human corneal endothelial cells have limited proliferative capacity in vivo, several laboratories have been successful in in vitro expansion of human corneal endothelial cells. Here we provide a comprehensive analysis of different substrates employed for in vitro cultivation of human corneal endothelial cells. Advances and emerging challenges with ex vivo cultured corneal endothelial layer for the ultimate goal of therapeutic replacement of dysfunctional corneal endothelium in humans with functional corneal endothelium are also presented.

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“…5 Thin carriers such as Descemet's membrane, 12 amniotic membrane, 13,14 gelatin membrane, [15][16][17][18] and cultured corneal endothelial cell sheets 19,20 are difficult to handle. Fresh native corneas denuded of their endothelium have also been used as carriers for in vitro studies 4,[21][22][23][24][25][26] or for transplantation in rats, 27,28 mouse, 29 rabbits, 30 cats, 31,32 and monkeys. [33][34][35] However, prolonged periods of culture are not possible with fresh corneas because of the risk for contamination of the endothelium by proliferating native epithelium of the living carrier.…”

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confidence: 99%

Transplantation of a Tissue-Engineered Corneal Endothelium Reconstructed on a Devitalized Carrier in the Feline Model

Proulx

Bensaoula

Nada

et al. 2009

Invest. Ophthalmol. Vis. Sci.

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Properties of Corneas Reconstructed with Cultured Human Corneal Endothelial Cells and Human Corneal Stroma

Abstract: HCEC on the tissue-engineered corneas have morphology and cellular density similar to HCEC in vivo, whereas the pump function of the tissue-engineered corneas was lower than in normal corneas.

Cited by 21 publications

References 18 publications

Cornea and Ocular Surface Treatment

Cornea and Ocular Surface Treatment

Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium

Transplantation of a Tissue-Engineered Corneal Endothelium Reconstructed on a Devitalized Carrier in the Feline Model

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