Transplantation of a Tissue-Engineered Corneal Endothelium Reconstructed on a Devitalized Carrier in the Feline Model

Proulx, Stéphanie; Bensaoula, T.; Nada, Ossama; Audet, Caroline; Uwamaliya, J.; Devaux, Ange`le; Allaire, Guy; Germain, Lucie; Brunette, Isabelle

doi:10.1167/iovs.08-2793

Cited by 49 publications

(34 citation statements)

References 52 publications

(53 reference statements)

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“…TE-HCE transplants were capable of recovering the corneal transparency of PCEP rabbits, maintaining the transparency of corneas for longer than previously reported (Aboalchamat et al, 1999;Ishino et al, 2004;Fu et al, 2006;Koizumi et al, 2008;Fan et al, 2009b;2010b;Proulx et al, 2009). The corneas of TE-HCE eyes became thinner and thinner with the time, while the corneas of PCEP and PKP eyes remained almost unchanged in thickness.…”

Section: Discussionmentioning

confidence: 62%

“…Diverse types of cells and various scaffold carriers have been combined previously and a therapeutic efficiency of about one week of maintenance of corneal transparency has been attained in rabbit (Aboalchamat et al, 1999;Ishino et al, 2004;Koizumi et al, 2008;Fan et al, 2009b;Proulx et al, 2009). Unfortunately, all the previous trials fell well short of a clinical therapy for PCEP; the immortalized seed cells were either potentially tumorigenic or limited in number after primary culture or 4-5 rounds of subculture (Fan et al, 2007;2009a).…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy

Fan

Zhao

et al. 2011

J. Zhejiang Univ. Sci. B

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Abstract:To evaluate the therapeutic efficiency of tissue-engineered human corneal endothelia (TE-HCEs) on rabbit primary corneal endotheliopathy (PCEP), TE-HCEs reconstructed with monoclonal human corneal endothelial cells (mcHCECs) and modified denuded amniotic membranes (mdAMs) were transplanted into PCEP models of New Zealand white rabbits using penetrating keratoplasty. The TE-HCEs were examined using diverse techniques including slit-lamp biomicroscopy observation and pachymeter and tonometer measurements in vivo, and fluorescent microscopy, alizarin red staining, paraffin sectioning, scanning and transmission electron microscopy observations in vitro. The corneas of transplanted eyes maintained transparency for as long as 200 d without obvious edema or immune rejection. The corneal thickness of transplanted eyes decreased gradually after transplanting, reaching almost the thickness of normal eyes after 156 d, while the TE-HCE non-transplanted eyes were turbid and showed obvious corneal edema. The polygonal corneal endothelial cells in the transplanted area originated from the TE-HCE transplant. An intact monolayer corneal endothelium had been reconstructed with the morphology, cell density and structure similar to those of normal rabbit corneal endothelium. In conclusion, the transplanted TE-HCE can reconstruct the integrality of corneal endothelium and restore corneal transparency and thickness in PCEP rabbits. The TE-HCE functions normally as an endothelial barrier and pump and promises to be an equivalent of HCE for clinical therapy of human PCEP.

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Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy

Fan

Zhao

et al. 2011

J. Zhejiang Univ. Sci. B

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“…Tissue-engineered feline corneal endothelium using cultured feline CECs seeded onto devitalized cadaver human stromal cornea achieved a cell density of 2272Ϯ344 cells/ mm 2 and expressed characteristic function-related markers such as Na ϩ K ϩ -ATPase and ZO-1 (105). A subsequent report showed functional success when the tissue-engineered feline corneal endothelium, reconstructed on a devitalized human stromal carrier, was transplanted into a feline model (104). Elsewhere, Honda et al (106), using cultured HCECs seeded onto fresh human corneal stromal discs, showed promising results when HCECs-populated stromal disc was transplanted into a rabbit model.…”

Section: Prospects For Corneal Endothelial Transplantation Using Cultmentioning

confidence: 97%

“…It is well established that HCECs retain their capacity to proliferate in vitro (30,64,97,104), and their growth dynamics are now better understood (29,42,76). Although primary HCECs cannot be propagated indefinitely, in the context of tissue-engineered corneal endothelial grafts, the use of nontransformed cells is advantageous over the use of transfected or immortalized HCEC-lines in the uncontrollable CEC growth observed (42).…”

Section: A Feasible Future For Tissue-engineered Corneal Endothelial mentioning

confidence: 99%

Human Corneal Endothelial Cell Expansion for Corneal Endothelium Transplantation: An Overview

Peh

Beuerman²,

Colman³

et al. 2011

Transplantation

219

224

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The monolayer of cells forming the human corneal endothelium is critical to the maintenance of corneal transparency and is not known to regenerate in vivo. Thus, dysfunction of these cells constitutes the most often cited reasons for the 150,000 or so corneal transplants performed yearly. Although current corneal transplantation is more than 90% successful at 1 year, longer term results are not as encouraging with approximately 70% success at 5 years. Nonimmunologic graft failure and allograft endothelial rejection are the main problems. Furthermore, the global shortage of donor corneas greatly restricts several corneal transplantations performed. With advances in understanding corneal endothelial cell biology, it is now possible to cultivate human corneal endothelial cells (HCECs) in vitro, thus providing new opportunities to develop novel tissue-engineered human corneal endothelium. This review will provide an overview of (a) the characteristics of human corneal endothelium; (b) past and present HCECs isolation and culture protocols; (c) various potential carriers for the generation of tissue-engineered corneal endothelium, together with some of the functional studies reported in various animal models; and (d) the current rapid advancements in surgical techniques for keratoplasty. A successful combination of tissue-engineered human corneal endothelium coupled with innovative and groundbreaking surgical procedures will bridge basic research involving cultured HCECs, bringing it from bench to bedside.

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“…If positioning donor tissue in its anatomical position would not even be necessary, the concept of using a carrier to implant ex-vivo cultured endothelial cells into the recipient anterior chamber may become an ever more realistic approach [74][75][76][77][78].…”

Section: Future Perspectivesmentioning

confidence: 99%

Endothelial keratoplasty: DSEK/DSAEK or DMEK - the thinner the better?

Dapena

Ham

Melles

2009

Current Opinion in Ophthalmology

258

170

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Transplantation of a Tissue-Engineered Corneal Endothelium Reconstructed on a Devitalized Carrier in the Feline Model

Abstract: This study provides evidence for the short-term (7-day) anatomic and functional success of corneal transplantation with a tissue-engineered corneal endothelium reconstructed on a devitalized carrier.

Cited by 49 publications

References 52 publications

Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy

Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy

Human Corneal Endothelial Cell Expansion for Corneal Endothelium Transplantation: An Overview

Endothelial keratoplasty: DSEK/DSAEK or DMEK - the thinner the better?

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