Properties of bioflocculant-producing bacteria for high flocculating activity efficiency

Alias, Jamsari; Hasan, Hassimi Abu; Abdullah, Siti Rozaimah Sheikh; Othman, Ahmad Razi

doi:10.1016/j.eti.2022.102529

Cited by 5 publications

(5 citation statements)

References 27 publications

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“…Bio occulants are microbial extracellular polymeric substances produced as secondary metabolites, that have the capacity to cause occulation in a given medium (Dih et al, 2019). They are produced as a result of cell breakdown and cell secretion by bacteria, fungi and algae, among other microbes (Alias et al, 2022) . Bio occulants also called biocoagulants (Kurniawan et al, 2022) aggregate particles and remove these particles from the liquid medium in which they were hitherto suspended.…”

Section: Introductionmentioning

confidence: 99%

“…Bio occulants are composed of cellulose, proteins, nucleic acids, lipids, glycoproteins and polysaccharides, which render them readily biodegradable and less injurious to the environment (Alias et al, 2022). They are polymeric substances produced during the bacterial cell growth phase (Tsilo et al, 2022), and each monomer contributes to the overall characteristics and mechanisms of action of the bio occulant.…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of a novel “glyco-organochlorine” bioflocculant produced from Pseudomonas aeruginosa strain F29 isolated from pig fecal matter collected from a mixed animal farm in Ibadan, Oyo State, Nigeria

Okorie,

Ogunjobi

2024

Preprint

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Antibiotic resistance has reached global proportions, and the discovery of effective alternatives to the common antibiotics currently in use, could aid in solving this problem. The aim of this study was to characterise a bioflocculant produced from Pseudomonas aeruginosa strain F29, accession number OQ734844, with 69% flocculating activity and which had been observed to possess biocontrol activity against Staphylococcus aureus SO183 at concentrations of 0.090 g/L and 0.150 g/L and against identified Pseudomonas aeruginosa at a concentration of 0.150 g/L. Pseudomonas aeruginosa strain F29, was isolated from pig fecal matter collected from a mixed animal farm in Ibadan, Nigeria via the pour plate method, identified through biochemical tests and confirmed through molecular studies. Fourier transform infrared (FTIR) spectroscopy of the bioflocculant revealed the presence of carboxyl, aromatic alcohol, alkene, halo and polysulfide functional groups. Scanning electron microscopy (SEM) revealed a clumped and flaky bioflocculant surface while energy dispersive X-ray spectroscopy (EDX) revealed the main chemical elements were chlorine (56.00%), carbon (20.50%), sodium (12.50%), oxygen (4.00%), and phosphorus (3.00%). High performance liquid chromatography (HPLC) and mass spectrometry (MS) revealed that the bioflocculant consisted of carbohydrate sugar moieties of glucose, galactose, inositol and mannose, D-ribose, arabinose, rhamnose and xylose; each with varied peaks. The phenolic sulfuric acid method showed that the concentration of these sugars was 0.0059 g/L. The bioflocculant was a polymeric compound composed mainly of carbohydrates and organochlorines, possibly known as a “glyco-organochlorine.” From available documentation, this is the first report of a “glyco-organochlorine” bioflocculant.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterisation of a novel “glyco-organochlorine” bioflocculant produced from Pseudomonas aeruginosa strain F29 isolated from pig fecal matter collected from a mixed animal farm in Ibadan, Oyo State, Nigeria

Okorie,

Ogunjobi

2024

Preprint

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“…The removal of colloidal particles mediated by flocculants is an important procedure in many industrial fields such as tap water purification and wastewater treatment, dyes and textiles, food and beverage. Flocculants are generally divided into three different categories: inorganic, organic, and bioflocculants (Alias et al, 2022). Compared with conventional chemical inorganic and organic flocculants, microbial bioflocculants, the macromolecules secreted by microorganisms during growth and lysis, are considered to be ecosystem-friendly due to their non-toxic and biodegradable properties.…”

Section: Introductionmentioning

confidence: 99%

A bioflocculant from Corynebacterium glutamicum and its application in acid mine wastewater treatment

Liu

Zeng²,

Yang³

et al. 2023

Front. Bioeng. Biotechnol.

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Although many microorganisms have been found to produce bioflocculants, and bioflocculants have been considered as attractive alternatives to chemical flocculants in wastewater treatment, there are few reports on bioflocculants from the safe strain C. glutamicum, and the application of bioflocculants in acid wastewater treatment is also rare attributed to the high content of metal ions and high acidity of the water. In this study, a novel bioflocculant produced by Corynebacterium glutamicum Cg1-P30 was investigated. An optimal production of this bioflocculant with a yield of 0.52 g/L was achieved by Box–Behnken design, using 12.20 g/L glucose, 4.00 g/L corn steep liquor and 3.60 g/L urea as carbon and nitrogen source. The structural characterization revealed that the bioflocculant was mainly composed of 37.50% neutral sugar, 10.03% uronic acid, 6.32% aminosugar and 16.51% protein. Carboxyl, amine and hydroxyl groups were the functional groups in flocculation. The biofocculant was thermally stable and dependent on metal ions and acidic pH, showing a good flocculating activity of 91.92% at the dosage of 25 mg/L by aid of 1.0 mM Fe3+ at pH 2.0. Due to these unique properties, the bioflocculant could efficiently remove metal ions such as Fe, Al, Zn, and Pb from the real acid mine wastewater sample without pH adjustment, and meanwhile made the acid mine wastewater solution become clear with an increased neutral pH. These findings suggested the great potential application of the non-toxic bioflocculant from C. glutamicum Cg1-P30 in acid mine wastewater treatment.

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“…The major source of water pollution is the discharge of effluents from industries such as coal mines [3]. Mine wastewater degrades the quality of water in different water bodies, it imposes a health risk to humans and animals and has a negative impact on the environment [4]. Waterborne diseases kill more than 1.8 million people and cause about 4 billion cases of illness yearly due to consumption of contaminated water [5].…”

Section: Introductionmentioning

confidence: 99%

“…Extensive research has been done on bioflocculant-producers from ecological niches such as sludge and soil [4,20]. However, the constant pressure to discover novel bioflocculants has recently necessitated the search for bioflocculant-producers from unique niches such as marine environments [21].…”

Section: Introductionmentioning

confidence: 99%

Isolation of a Marine Bacterium and Application of Its Bioflocculant in Wastewater Treatment

Maliehe

Moganedi

Masoko

et al. 2022

Microbiology Research

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Bioflocculation has become the method of choice in wastewater treatment because of its effectiveness, environmental friendliness and innocuousness to humans. In this study, the bioflocculant-producing bacterium was isolated and its bioflocculant was used in wastewater treatment. The isolate was identified by 16S rRNA gene sequencing analysis. Its culture conditions (inoculum size, carbon and nitrogen sources, pH, temperature and time) were optimised using the one-factor-at-a-time assay. The cytotoxicity of the bioflocculant was assessed on human colorectal adenocarcinoma cells (Caco2) by tetrazolium-based colorimetric method. The ability of the bioflocculant to reduce biochemical oxygen demand (BOD) and chemical oxygen demand (COD) in wastewater was evaluated using Jar test. The bacterium was identified as Bacillus subtilis CSM5 and the maximum flocculating activity of 92% was observed when fructose and urea were used as nutrients and the culture conditions were adjusted to 30 °C, pH 9, 160 rpm and 72 h of incubation. Caco2 exhibited 90% viability when the highest bioflocculant concentration of 200 µg/µL was used. The reduction of BOD and COD was achieved at 59 ± 3.1 and 75 ± 0.4%, respectively. In conclusion, B. subtilis CSM5 is a good candidate for bioflocculant production and its bioflocculant has good potential for use in wastewater treatment.

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Properties of bioflocculant-producing bacteria for high flocculating activity efficiency

Cited by 5 publications

References 27 publications

Characterisation of a novel “glyco-organochlorine” bioflocculant produced from Pseudomonas aeruginosa strain F29 isolated from pig fecal matter collected from a mixed animal farm in Ibadan, Oyo State, Nigeria

Characterisation of a novel “glyco-organochlorine” bioflocculant produced from Pseudomonas aeruginosa strain F29 isolated from pig fecal matter collected from a mixed animal farm in Ibadan, Oyo State, Nigeria

A bioflocculant from Corynebacterium glutamicum and its application in acid mine wastewater treatment

Isolation of a Marine Bacterium and Application of Its Bioflocculant in Wastewater Treatment

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