Properties of amino acid neurotransmitter receptors of embryonic cortical neurons when activated by exogenous and endogenous agonists

Blanton, MG; Kriegstein, Arnold R.

doi:10.1152/jn.1992.67.5.1185

Cited by 44 publications

(25 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous ligand binding and electrophysiological studies have demonstrated the presence of functional AMPA and NMDA receptors in the forebrain of turtles, but no specific information has been available on the regional or cellular localization of NMDA and AMPA subunits in the basal ganglia of reptiles [Young et al, 1990;Larson-Prior et al, 1991;Blanton and Kriegstein, 1992]. In the present study we have characterized the distribution of several AMPA and NMDA receptor type subunits in the basal ganglia of turtles.…”

Section: Discussionmentioning

confidence: 77%

“…Although we did not demonstrate the presence of NMDAR1 receptor subunits in turtle brain, several considerations argue that such subunits are present and likely to be found in the same neurons that we found to possess NMDAR2A/B subunits. First, functional NMDA receptors cannot form in the absence of the NMDAR1 subunit, and physiological and ligand binding studies demonstrate the presence of functional NMDA receptors in turtles [Young et al, 1990;Larson-Prior et al, 1991;Blanton and Kriegstein, 1992]. Secondly, an NMDAR1 subunit has been demonstrated in chick [Kurosawa et al, 1994] and in a lizard species [Rodger et al, 1999], as well as in Xenopus laevis and a teleost species [Soloviev et al, 1997;Dunn et al, 1998].…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, because functional NMDA receptors cannot form without the NMDAR1 subunit [Seeburg, 1993;Hollman and Heinemann, 1994], because functional NMDA receptors have been demonstrated in turtle nervous system by electrophysiological means [Larson-Prior et al, 1991;Blanton and Kriegstein, 1992] and because an NR1 subunit has been demonstrated in an avian species [Kurosawa et al, 1994] and in a lizard species [Rodger et al, 1999], it seems likely that NMDAR1 subunits are present in turtle brain. Our failure to detect these subunits with a monoclonal antibody directed against rat NMDAR1 seems likely to reflect the fact that the NMDAR1 epitope against which this antibody is directed is sufficiently different in turtles that the antibody against a mammalian NMDAR1 does not recognize turtle NMDAR1.…”

Section: Methodsmentioning

confidence: 99%

“…The cortical inputs arise from the surface structure known as the dorsal cortex and from the subcortical pallial structure known as the dorsal ventricular ridge [Hall and Ebner, 1970;Brauth and Kitt, 1980;Hoogland and Vermeulen-Vanderzee, 1989;Gonzalez et al, 1990;Ulinski, 1990], whereas the thalamic inputs arise from what appear to be the nonspecific intralaminar nuclei of the dorsal thalamus in turtles and other reptiles [Parent, 1976;Balaban and Ulinski, 1981;Veenman et al, 1997]. Some prior data suggest that cortical projection neurons in turtles are glutamatergic [Larson-Prior et al, 1991;Reiner, 1991;Blanton and Kriegstein, 1992], but little is otherwise known about the presynaptic and postsynaptic neurotransmitter organization of the cortical and thalamic input to the basal ganglia in turtles. Information on the preand postsynaptic localization of glutamate and glutamate receptors in the basal ganglia projection systems in turtles is important because it provides further insight into the evolution of the basal ganglia and its afferent systems.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Immunohistochemical Localization of NMDA- and AMPA-Type Glutamate Receptor Subunits in the Basal Ganglia of Red-Eared Turtles

Fowler

Medina²,

Reiner³

1999

Brain Behav Evol

View full text Add to dashboard Cite

Corticostriatal and thalamostriatal projection systems have been shown to utilize glutamate as a neurotransmitter in mammals and birds. Although corticostriatal and thalamostriatal projection systems have been demonstrated in turtles, it is uncertain whether they too use glutamate as their neurotransmitter. Immunohistochemical localization of glutamate and of NMDA- and AMPA-type ionotropic glutamate receptor subunits (NMDAR2A/B, GluR1, GluR2/3, and GluR4) were used to address this issue. Numerous medium-sized neurons that were rich in NMDAR2A/B and GluR2/3 were observed in the striatal part of the basal ganglia of red-eared turtles. Smaller numbers of medium-sized neurons and some large neurons rich in the GluR1 and GluR4 subunits were also observed in the striatum. The striatal neuropil was notably rich in GluR1, GluR2/3 and NMDAR2A/B subunits. The pallidal region was specifically rich in large neurons possessing GluR4 subunits. Consistent with the glutamate receptors on striatal and pallidal neurons, sources of input to the striatum and pallidum in turtle such as the dorsomedial and dorsolateral thalamic nuclei (which appear to correspond to intralaminar thalamic nuclei), telencephalic pallial cell groups, and the apparent subthalamic nucleus homologue were rich in glutamatergic neurons. The results show that the thalamostriatal, corticostriatal and subthalamo-pallidal projection systems of turtles are glutamatergic and that similar basal ganglia cell types in turtles and mammals have largely similar glutamate receptor characteristics.

show abstract

Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Immunohistochemical Localization of NMDA- and AMPA-Type Glutamate Receptor Subunits in the Basal Ganglia of Red-Eared Turtles

Fowler

Medina²,

Reiner³

1999

Brain Behav Evol

View full text Add to dashboard Cite

show abstract

“…Contributions by NMDA receptors are disproportionately large during early postnatal periods in most brain regions where they have been examined [Tsumoto et al, 1987;Ben-Ari et al, 1989;Yuste and Katz, 1991;Agmon and O'Dowd, 1992;Blanton and Kriegstein, 1992;Carmignoto and Vicini, 1992;Ramoa and McCormick, 1994;McLean et al, 1995]. In addition, during early postnatal development in the rat, glutamatergic transmission can be mediated solely by NMDA receptors in the hippocampus, in thalamocortical connections and in the optic tectum [Liao et al, 1995;Durand et al, 1996;Wu et al, 1996;Ben-Ari et al, 1997;Isaac et al, 1997].…”

Section: Introductionmentioning

confidence: 99%

Delayed Postnatal Development of NMDA Receptor Function in Medium-Sized Neurons of the Rat Striatum

Hurst

Cepeda²,

Shumate³

et al. 2001

Dev Neurosci

View full text Add to dashboard Cite

During early postnatal development, the N-methyl-D-aspartate (NMDA) subtype of glutamate receptor plays a dominant role in excitatory amino acid-mediated synaptic transmission in essentially every brain region that has been examined. In contrast, we have found that in the rat striatum, NMDA receptor-mediated current develops later in the medium-sized neurons (MSNs) than currents mediated by activation of non-NMDA receptors. MSNs were identified using infrared video microscopy, and voltage-clamped in a slice preparation using the whole-cell patch-clamp technique. Intrastriatal stimulation was used to evoke excitatory synaptic currents from slices in animals ranging in age from postnatal day (PND) 5 to 60. Though most cells from animals younger than PND 10 failed to respond to synaptic stimulation, postsynaptic responses were occasionally evoked in cells as young as PND 5. Synaptic currents from cells between PNDs 5 and 7 had a significant contribution due to activation of non- NMDA receptors, as evidenced by sensitivity to the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione and rapidly rising and falling response components. The relative contribution of NMDA receptors increased approximately twofold from the first to the third postnatal week; no further change was observed through PND 60. At the same ages that the NMDA receptors contributed maximally to the synaptic current, the decay time constant of the NMDA receptor-mediated current decreased significantly. The increasing weight of NMDA receptor-mediated current may reflect a change in the number of functional receptors at the synapse since there was no apparent change in the voltage dependence of the current. To more completely examine receptor function early in postnatal development, NMDA and kainate were applied either iontophoretically or in the bath. Iontophoretic application of NMDA onto cells obtained from rats between PNDs 3 and 5 only occasionally evoked current, provided that the membrane was held at depolarized potentials to remove the Mg²⁺ block. In contrast, application of kainate consistently evoked a response from cells of the same age group. Bath application of the same agonists provided similar results. Taken together, the present experiments demonstrate that striatal non-NMDA receptor-mediated currents are more mature than NMDA receptor-mediated currents early in development.

show abstract

Orchestration of neuronal migration by activity of ion channels, neurotransmitter receptors, and intracellular Ca2+ fluctuations

1998

View full text Add to dashboard Cite

The real‐time observation of cell movement in acute cerebellar slices reveals that granule cells alter their shape concomitantly with changes in the mode and rate of migration as they traverse different cortical layers. Although the origin of local environmental cues responsible for these position‐specific changes in migratory behavior remains unclear, several signaling mechanisms involved in controlling granule cell movement have emerged. The onset of one such mechanism is marked by the expression of voltage‐gated ion channels and neurotransmitter receptors in postmitotic cells prior to the initiation of their migration. Granule cells start their radial migration after the expression of N‐type Ca2+ channels and the N‐methyl‐D‐aspartate subtype of glutamate receptors on the plasmalemmal surface. Blockade of the channel or receptor activity significantly decreases the rate of cell movement, indicating that the activation of these membrane constituents provides an essential signal for the translocation of granule cells. Another signal that controls the rate of cell migration is embedded in the combined amplitude and frequency components of Ca2+ fluctuations in the somata of migrating granule cells. Interestingly, each phase of Ca2+ fluctuation controls a separate phase of saltatory movement in the granule cells: The cells move forward during the phase of transient Ca2+ elevation and remain stationary during the troughs. Consequently, the changes in the amplitude and frequency components of Ca2+ fluctuations directly affect granule cell movement: Reducing the amplitude or frequency of Ca2+ fluctuations slows down the speed of cell movement, while the enhancement of these components accelerates migration. These findings suggest that signaling molecules present in the local cellular milieu encountered on the migratory route control the shape and motility of granule cells by modifying Ca2+ fluctuations in the soma through the activation of specific ion channels and neurotransmitter receptors. © 1998 John Wiley & Sons, Inc. J Neurobiol 37: 110–130, 1998

show abstract

Properties of amino acid neurotransmitter receptors of embryonic cortical neurons when activated by exogenous and endogenous agonists

Cited by 44 publications

References 0 publications

Immunohistochemical Localization of NMDA- and AMPA-Type Glutamate Receptor Subunits in the Basal Ganglia of Red-Eared Turtles

Immunohistochemical Localization of NMDA- and AMPA-Type Glutamate Receptor Subunits in the Basal Ganglia of Red-Eared Turtles

Delayed Postnatal Development of NMDA Receptor Function in Medium-Sized Neurons of the Rat Striatum

Orchestration of neuronal migration by activity of ion channels, neurotransmitter receptors, and intracellular Ca2+ fluctuations

Contact Info

Product

Resources

About