Properties of a 4-ene-3-ketosteroid-5α-reductase in cell extracts of the intestinal anaerobe, Eubacterium sp. strain 144

Characterization and application of a novel cholesterol-reducing anaerobe, Eubacterium coprostanoligenes ATCC 51222

“…In TAPS buffer, however, CR activity was inhibited at pH 7.5 but recovered at higher pH. The dramatically different responses to different buffers and pH values also were reported in other studies about steroid double bond-reductases in Eubacteriiim (Glass et al 1991;. Evidently, different chemical compositions of the buffer systems contribute to the difference in enzyme activities at the same pH.…”

Conceptualizing the Vertebrate Sterolbiome

“…Bacteria expressing 16␣-dehydroxylase activity were isolated and described as Gram-positive non-spore-forming obligate anaerobes that are nonmotile, with characteristics nearly identical to those of Eggerthella lenta, including in the case of strain 146 stimulation by addition of L-arginine, but did not reduce nitrate (121). Partial purification and characterization of 16␣-dehydroxylase have been performed (122)(123)(124)(125). Further work is needed to identify and characterize the gene(s) encoding 16␣-dehydroxylase.…”