Properties and Partial Protein Sequence of Plant Annexins

Abstract

We have examined the characteristics of Ca2"-dependent phospholipid-binding proteins (annexins) Iongiflorum. Immunolocalization at the light microscope level suggests that these proteins are predominantly confined to the nongranular zone at the tube tip, a region rich in secretory vesicles. Our hypothesis that plant annexins mediate exocytotic events is supported by the finding that p23, p33, and p35 bind to these secretory vesicles in a Ca2"-dependent manner.

Annexins of Plant Cells

“…So far there is no evidence for a plant annexin homologous to tlhe heterotetrameric form of annexin 11. The in vitro binding of the 33-and 35-kD maize annexins to F-actin has been tested, and there is no detectable binding under the conditions of the assay, nor is there any immunological detection of annexins in the detergent-insoluble cytoskeletal fractions (Blackbourn et al, 1992). There is also no detectable binding of the 35-kD green pepper annexin to animal F-actin (Hoshino et al, 1995).…”

“…Immunolocalization studies of plant annexins have been carried out in pea plumules and roots (Clark et al, 1992), Lilium pollen tubes (Blackbourn et al, 1992), corn roots (Clark et al, 1994), and fern rhizoids (Clark et al, 1995). The staining pattern in pea and corn seedlings reveals that annexins are highly concentrated in secretory cell types, such as the outer cells of the root cap, epidermal cells, and developing xylem and phloem cells (Clark et al, 1992(Clark et al, , 1994.…”

Immunolocalization of a novel annexin‐like protein encoded by a stress and abscisic acid responsive gene in alfalfa

“…Some of the plant annexins have been shown to possess intrinsic phosphodiesterase activity (Calvert et al, 1996;McClung et al, 1994). Annexins were also suggested to play various roles in secretion and ripening processes (Blackbourn et al, 1992;Clark et al, 1992;Clark and Roux, 1995). Recently, an annexin-like protein has been identified from Arabidopsis thaliana which may be involved in the response to oxidative stress (Gidrol et al, 1996).…”

Characterization and gene expression of an annexin during fruit development in Capsicum annuum

“…In animal tissues, they have been shown to be implicated in multiple aspects of cell biology including regulation of membrane trafficking, transmembrane channel activity, transduction in mitogenic signal and settlement of cell-matrix interactions [1]. However, the precise biological functions of annexins are still unknown, and concerning their occurrence and implication in plant cells, only very liitle information is available [2]: among the hypotheses, it has been proposed that annexins may function in the Golgimediated secretion of wall polysaccharides in plant cells [3]. We have isolated the first full-length cDNA encoding a plant *Corresponding author.…”