Characterization and gene expression of an annexin during fruit development in Capsicum annuum

Abstract

Several lines of evidence indicate that annexins, as calcium-dependent pbospbolipid-binding proteins, are involved in a variety of plant cellular processes. We were interested in determining if annexins are implicated in the highly regulated fruit development of bell pepper. By differential screening of several cDNA libraries, we isolated a full-length cDNA of 1180 bp encoding an annexin. Northern blot analyses show a differential expression pattern of the transcripts during the early stages of development and during ripening. Immunoblots using antiserum raised against p33/p35 from maize reveal that crossreactive polypeptides of about 30 kDa are present at each stage of fruit development in bell pepper. We partially purified the annexins from seedlings and green fruits. At least one annexin of 32 kDa is present in these plant tissues.

cDNA Isolation and Gene Expression of the Maize Annexins p33 and p35

“…Very recently a full-length cDNA was reported for an annexin from bell pepper (Proust et al, 1996), which is most similar to the amino acid sequence predicted from the alfalfa partial cDNA, and slightly closer to the maize p33 sequence than to p35 (Fig. 2).…”

“…The longest cDNA clone was sequenced and its Comparison of annexin identities. The percentage identities were calculated pairwise for the amino acid sequences predicted from coding regions of full-length annexin cDNAs from maize (ZMANN33, ZMANN35), bell pepper (CAANN; Proust et al, 1996), and humans (HSANNXIII; Wice and Gordon, 1992), and partial cDNAs from alfalfa (MSANN; Pirck et al, 1994) and strawberry (FAANN; Wilkinson et al, 1995). The identities were calculated using the PALIGN program in PCGENE (Intelligenetics).…”

Immunolocalization of a novel annexin‐like protein encoded by a stress and abscisic acid responsive gene in alfalfa

“…The deduced amino acid sequence of AnnMs2 is shown in Figure 1 in comparison with the amino acid sequences of Medicago sativa (Pirck et al, 1994), Capsicum annuum (Proust et al, 1996), Arabidopsis thaliana (Gidrol et al, 1996), Zea mays p33 and p35 annexins (Battey et al, 1996). AnnMs2 possesses the common primary structure of annexins: a short N-terminal tail and the C-terminal core composed of four repeats of 70-75 amino acids, but it is more difficult to predict the precise position of the repeats than in the case of animal annexins.…”

“…This type of Ca 2ϩ -binding site has been defined on the basis of the crystallographic analysis of annexin I and V (Weng et al, 1993). (M.s;Pirck et al, 1994), Arabidopsis thaliana (A.th;Gidrol et al, 1996), Capsicum annuum (C.an;Proust et al, 1996) and Zea mays (Z.m33 and Z.m35;Battey et al, 1996). The repeat sequences are bold and residues external to the four repeats are in italic.…”

“…(Blackbourn et al, 1992;Boustead et al, 1989;Seals et al, 1994). Recently, a partial annexin cDNA sequence has been obtained from Medicago sativa (Pirck et al, 1994) and strawberry (Wilkinson et al, 1995), and full-length cDNA sequences from Capsicum annuum (Proust et al, 1996), Zea mays (Battey et al, 1996) and Arabidopsis thaliana (Gidrol et al, 1996). The predicted amino acid sequences of plant annexins show a high degree of similarity to each other, although they are distinct from the mammalian annexins.…”