Propagation of Picrorhiza kurroa Royle ex Benth: An important medicinal plant of Western Himalaya

Patial, Vanita; Devi, Kiran; Sharma, Madhu; Bhattacharya, Amita; Ahuja, Paramvir Singh

doi:10.5897/jmpr12.115

Cited by 28 publications

(16 citation statements)

References 32 publications

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“…One of such plant is Picrorhiza kurroa Royle ex. Benth belonging to family Scrophulariaceae which is endemic to Himalayan region (Patial et al, 2012). The plant grows from Kashmir to Sikkim at an altitude of 3,000-5,000 meters.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and callus proliferation in Picrorhiza kurroa Royle ex. Benth

Mahajan¹,

Kapoor²

2016

JEBAS

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Picrorhiza kurroa Royle ex. Benth is an endangered and important medicinal plant of alpine Himalayas. Developing an efficient protocol for its mass multiplication is essential to meet the requirements of pharmaceutical industries and also in conservation of this plant under its natural habitat. Present study was undertaken to develop a protocol for in vitro mass multiplication of P. kurroa. Result of study revealed that highest frequency of shoot regeneration was achieved on Murashige and Skoog's basal medium supplemented with 1.0mg/l BAP, 0.5 mg/l Kn and 1.0mg/l GA3, while the best rooting was observed in MS medium supplemented with 2.5 mg/l IBA. MS medium supplemented with 3.0 mg/l 2,4-D resulted in highest frequency of embryogenic callus. Callus inoculated on MS media supplemented with BAP and IAA resulted in both shoot and root formation while the callus on MS media supplemented with NAA and IBA resulted only root formation. The somatic embryos were established from callus on MS medium supplemented 2.5 mg/l 2,4-D after four weeks. MS medium containing 1.0 mg/l BAP and 1.0 mg/l GA3 resulted into shoots from well developed somatic embryos. This protocol will provide a system for the germplasm conservation in P. kurroa by multiplication and regeneration of true to type plants.

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Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and callus proliferation in Picrorhiza kurroa Royle ex. Benth

Mahajan¹,

Kapoor²

2016

JEBAS

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“…In a parallel study, seed progenies of these germplasm were raised under ex-situ conditions. Its reproductive cycle was also studied for successful domestication [40]. Classification Table 1.…”

Section: Threats and Conservationmentioning

confidence: 99%

Picrorhiza kurroa: An ethnopharmacologically important plant species of Himalayan region

Masood¹

2015

PAB

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Picrorhiza kurroa Royle ex Benth. commonly known as Kutki, belongs to family Scrophulariaceae. It is found in the Himalayan regions of China, Pakistan, India, Bhutan and Nepal. It is considered as an important medicinal plant which is mostly used in the traditional medicinal system for asthama, jaundice, fever, malaria, snake bite and liver disorders Different pharmacological activities of P. kurroa include anti-microbial, anti-oxidant, anti-bacterial, antimutagenic, cardio-protective, hepato-protective, anti-malarial, anti-diabetic, anti-inflammatory, anti-cancer, anti-ulcer and nephro-protective activities were recorded from this plant. So far, Iridoids (Picroside I and II), Cucurbitacins and Phenolic components are the different phytochemicals which are extracted from P. kurroa. The authentification of P. kurroa raw material for commercially available herbal/botanical products is essential and it is done by the DNA fingerprinting of P. kurroa. Because of the over-exploitation of P. kurroa for medicinal purposes, the conservational status of P. kurroa in different regions became endangered. It is the need of the hour to utilize different conservational strategies and save this medicinal wealth from extinction as it is widely used by the local people for curing different diseases and thus it cause immense pressure on the plant population.

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“…Biotechnological aids like in vitro tissue culture technique can be used along with the conventional methods of propagation for multiplication and conservation of this elite germplasm. Various studies have shown that in vitro tissue culture techniques increase the speed and scale of production of plantlets [2][3][4]. The growth conditions of plants grown under aseptic condition are different from that of field grown plants.…”

Section: Introductionmentioning

confidence: 99%

“…Roots and rhizomes are the rich source of its active constituents hepatoprotective picrosides; picroside-i and picroside-ii and other metabolites like picroside-iii, picroside-iv, apocynin, androsin, catechol and kutkoside, etc. [2][3][4]. Propagation of P. kurroa plants in nature take place through its roots or suckers (vegetative propagation) and seeds.…”

Section: Introductionmentioning

confidence: 99%

Use of Piriformospora indica as Potential Biological Hardening Agent for Endangered Micropropagated Picrorhiza kurroa Royel ex Benth

Das

Tripathi

Varma

2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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The rich plant biodiversity of Himalayan region is vanishing at an alarming rate due to urbanization and indiscriminate collection of important plants at a large scale from the wild for trade. Overexploitation of Picrorhiza kurroa, an alpine Himalayan medicinal plant for extraction of its active compounds has led to the reduction of its natural population. In vitro regeneration offers a noteworthy solution for the propagation of endangered medicinal plants. Successful acclimatization of in vitro plants with fungal endophyte Piriformospora indica reduces the mortality rate of in vitro plantlets due to transplantation shock. Root colonization with P. indica renders growth promotion and better adaptation of a wide range of plants. Present study focuses on the use of P. indica as a biological hardening agent for increasing the percentage survival of in vitro shoots. Survival of fungus treated biohardened in vitro plants was increased by 1.3 folds. Root number in 4 weeks old P. indica treated biohardened in vitro plants enhanced by 1.25 fold and facilitated its establishment under greenhouse conditions.

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Propagation of Picrorhiza kurroa Royle ex Benth: An important medicinal plant of Western Himalaya

Cited by 28 publications

References 32 publications

Somatic embryogenesis and callus proliferation in Picrorhiza kurroa Royle ex. Benth

Somatic embryogenesis and callus proliferation in Picrorhiza kurroa Royle ex. Benth

Picrorhiza kurroa: An ethnopharmacologically important plant species of Himalayan region

Use of Piriformospora indica as Potential Biological Hardening Agent for Endangered Micropropagated Picrorhiza kurroa Royel ex Benth

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