Propagation in Human Cells of a Filterable Agent from the ST Feline Sarcoma

Abstract

Nineteen lines of human fibroblasts were inoculated with a filtrate prepared from the ST feline sarcoma. Seven lines showed morphological alteration and released focus-forming activity for feline cells, 2 lines showed morphological alteration but did not release focus-forming activity, and 11 lines showed no morphological alteration and released no focus-forming agent. Morphologically altered cells appeared enlarged, hyper-refractile, and intensely stained by hematoxylin. They neither assumed a crisscross pattern nor piled up to form a visible focus. Time-lapse cinegraph showed that the morphologically altered cells did not divide and were motile. The fluid from two human fibroblast cultures, inoculated 4 and 14 weeks previously with the ST sarcoma filtrate, induced fibrosarcoma in newborn kittens. The cell-free transmission of the ST feline sarcoma between kittens and from kittens to newborn dogs and marmosets was recently demonstrated (2, 14). The propagation in human cell cultures of a filterable agent from this feline sarcoma is now described. MATERIALS AND METHODS Feline sarcoma filtrate. The Snyder-Theilen line of sarcoma (14) was used. A crude 33% tumor suspension in tris(hydroxymethyl)aminomethane(Tris)-acetate buffer, pH 7.0, was homogenized in a mortar with sterile sand. Seven volumes of phosphate-buffered saline were mixed with three volumes of the tumor homogenate. The mixture was centrifuged at 1,500 rev/min for 15 min, and the supernatant fluid was passed through an ultrafine glass filter. The filtrate was stored in l-ml samples at below-60 C. The integrity of the glass filter was checked with a 10-ml suspension of Staphylococcus aureus; no bacterium was found in the filtrate. Nutrient medium. Nutrient medium consisted of 20% fetal calf serum in Eagle basal medium supplemented with nonessential amino acids. Penicillin and streptomycin were added to 50 units and 50,g/ml. Cell cultures. Human fibroblast cultures were originated from embryonic or sarcomatous tissues. Many of the human cell lines were gifts from the Naval Biological Laboratory, Oakland. A few were origi

Antibody to feline oncornavirus‐associated cell membrane antigen in neonatal cats

“…The isolation of an oncornavirus which induces fibrosarcomas in cats, dogs, rabbits, sheep and monkeys has been reported Deinhardt et al, 1970;Gardner et al, 1970;Snyder et al, 1970;Theilen et al, 1970;Theilen, 1971). This agent, which also induces morphological transformation in cat, dog, and human cells (Chang et al, 1970;Sarma et al, 1970), has been designated the feline sarcoma virus (FSV). The inoculation of kittens under 1 week of age with FSV usually results in the development of progressive malignant tumors which kill the host Within 20-40 days after virus injection Essex et al, 1971).…”

Could FIV zoonosis responsible of the breakdown of the pathocenosis which has reduced the European CCR5-Delta32 allele frequencies

“…Moreover, cat zoonoses can be transmitted to man [41,42] and the hypothesis of the role of FIV remains putative. However, the implication of a feline retrovirus could be plausible; indeed, three other species of feline retroviruses, feline foamy virus (FeFV), feline sarcoma virus (FeSV) and Feline leukemia virus (FeLV) can replicate in some human cell cultures with generally production of infectious virus and could sometimes produce morphological cell change [151,[180][181][182][183][184][185][186][187][188][189][190][191][192]. Moreover, cat horizontal transmission of FeLV by cat fleas has also been demonstrated [193] and FeSV can also induce malignant tumours in non felid mammalian including monkeys [194].…”