Pronuclear morphology evaluation for fresh in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles: a systematic review

Nicoli, Alessia; Palomba, Stefano; Capodanno, Francesco; Fini, Maria; Falbo, Angela; Sala, Giovanni Battista La

doi:10.1186/1757-2215-6-64

Cited by 26 publications

(20 citation statements)

References 55 publications

(244 reference statements)

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“…Early event assessment up to first cleavage of human embryos has been successfully correlated with subsequent embryonic development and/or implantation results [13][14][15]. But a complete picture of the morphometric changes within the oocyte before and after sperm injection for oocytes undergoing ICSI, together with the variability between oocytes, is still not clear.…”

Section: Introductionmentioning

confidence: 98%

Time-lapse videography of human oocytes following intracytoplasmic sperm injection: Events up to the first cleavage division

Liu

Chapple²,

Roberts

et al. 2014

Reproductive Biology

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Section: Introductionmentioning

confidence: 98%

Time-lapse videography of human oocytes following intracytoplasmic sperm injection: Events up to the first cleavage division

Liu

Chapple²,

Roberts

et al. 2014

Reproductive Biology

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“…To date, literature data about the correlation among zygote morphology, biological and clinical outcomes are inconclusive, mainly due to different methods used for PN scoring system, the time of PN observation, and the insemination procedure [10].…”

Section: Discussionmentioning

confidence: 99%

“…Since the preliminary observations on PN formation and morphology, several scoring systems were proposed mainly taking into account the PN size, the NPB position and alignment [10]. Although the scores have been correlated with improved embryo development as well as with increased pregnancy and implantation, to date clinical data about the relationship between zygote morphology and IVF outcomes are con icting [10]. One of the main limitation of literature data is the use of different zygote grading systems.…”

Section: Introductionmentioning

confidence: 99%

Pronuclear score improves prediction of implantation rate in ICSI cycles

Stigliani

Massarotti

Casciano

et al. 2020

Preprint

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Background: In assisted reproduction technology embryo competence is routinely evaluated on morphological criteria but their efficacy remains relatively low. Additional information could be obtained by evaluation of pronuclear (PN) morphology. Up to now controversial results have been reported about prognostic value of PN score. One of the main limitation of literature data is the use of different methods of PN classification. To this regard, in 2011 the ESHRE and Alpha Scientists in Reproductive Medicine defined three PN categories to standardize the zygote assessment. In this study we evaluated whether the consensus ESHRE-Alpha system for the pronuclear scoring could be an useful additional criterion to improve prediction of embryo implantation potential.Methods: This is a retrospective, longitudinal cohort study. We included 3004 zygotes from 539 women who underwent ICSI treatment at our Center between January 2014 and June 2019. The pronuclear were categorized as score 1: symmetrical, 2: non-symmetrical, 3: abnormal. A subset of 110 zygotes did not cleaved. On day 2-3 1163 embryos were transferred, 232 arrested, and 9 were cryopreserved. Among the 1490 embryos cultured up to day 5-7, 516 became blastocysts: 123 were transferred on day 5 and 393 were cryopreserved. Relationship between pronuclear score and cleavage rate, quality of embryos, blastocyst development, and implantation rate was evaluated by chi-square test. Multivariate regression analyses corrected the results for putative confounders (age of patient; infertility cause; cleavage-stage embryo morphology grade; day of transfer). Results: There was not significant difference in patients’ age, cleavage rate and embryo morphology among the three pronuclear score groups. No reduction of blastulation rates was found in score 2-3 (34%) groups respect to score 1 (35%). The pronuclear score 1-embryos had a higher implantation rate respect to score 2-3-ones (15% and 9%, respectively, P=0.0121; OR 0.46; 95% CI 0.25-0.76, P=0.004). Consistently, the pronuclear score remained predictive of implantation in top quality embryos (OR 0.51; 95% CI 0.28-0.87, P= 0.01).Conclusions: The consensus pronuclear score may be routinely included among criteria for embryo evaluation to increase patient’s chance of becoming pregnant.

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“…15 from 20 studies (75%) also showed its correlation to blastocyst formation and quality. [16] PN scoring of 3 were considered abnormal due to lack of nucleolar precursor body (NPB) or only have one NPB. Those embryos show inability to cleave on second or third day.…”

Section: Discssionsmentioning

confidence: 99%

Comparison of Time Lapse Embryo Morphokinetics Monitoring and Conventional Pronuclear Morphology Assessment to Determine Embryo Quality in Endometriosis Patients

Natadisastra¹

2016

Kme

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ObjectiveEndometriosis patients who underwent IVF program has poorer embryo quality, leads to low successive rate. In order to determine embryo quality, time lapse embryo monitoring and pronuclear (PN) morphology assessment were used to predict and select the most promising embryo. This research is expected to exhibit comparison the use of both methods in endometriosis patients in order to implement the most effective evaluation in future embryo implantation prognosis.MethodsThis cohort research was conducted to 72 embryos from 19 endometriosis patients who underwent IVF program in Yasmin Reproductive Clinic, Cipto Mangunkusumo Hospital. After ICSI was performed two-nucleated embryo later be monitored by its cleavage time and would be classified into categories for good quality embryo and other classes were considered poor quality embryo for time lapse monitoring. Then from time lapse monitoring, PN morphology were assessed regarding pronuclear scoring.ResultsBased on 72 embryos observation, we found that there was no significant difference between good and poor quality embryo based on PN scoring with p=0.185, and also between time-lapse morphokinetic monitoring and embryo quality with p=0.526. However, we found that there was a weak correlation between PN scoring and embryo quality with p=0.049 and r=0.233.ConclusionThere was no significant difference found between time lapse monitoring and PN scoring in endometriosis patients in order to determine embryo quality. However, there was a weak correlation between PN scoring and embryo quality. This might be due to small number of samples taken and visual bias during observation.

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Pronuclear morphology evaluation for fresh in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles: a systematic review

Cited by 26 publications

References 55 publications

Time-lapse videography of human oocytes following intracytoplasmic sperm injection: Events up to the first cleavage division

Time-lapse videography of human oocytes following intracytoplasmic sperm injection: Events up to the first cleavage division

Pronuclear score improves prediction of implantation rate in ICSI cycles

Comparison of Time Lapse Embryo Morphokinetics Monitoring and Conventional Pronuclear Morphology Assessment to Determine Embryo Quality in Endometriosis Patients

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