Abstract:Pronuclear morphology has been reported as a good tool for studying embryo development and euploidy. Comparing two groups of women with different aneuploidy risk, women more than 38 years old (n = 28) known to be at high risk of aneuploidy, and women under 30 years old (n = 35), this study investigated whether pronuclear morphology could be used routinely as an alternative to preimplantation genetic screening (PGS) in countries where PGS is prohibited. Pronuclear morphology was evaluated for 301 zygotes and re… Show more
“…In agreement with previously reported data [19], our analysis of nucleolar morphology showed no statistically significant differences in relation to maternal ages, both in clinical pregnancy and in ongoing pregnancy/deliveries groups. This result is in contrast with recently published data showing a correlation between pronuclear morphology and maternal ages [15]. We believe that the discrepancy could be related to the different sample size and to the different maternal age between our patients and those analyzed by Maille and coworkers.…”
Section: Discussioncontrasting
confidence: 99%
“…An important contribution in the evaluation of embryo quality seems to come from the pronuclear and nucleolar characteristics, proposed as an indicator of embryo development and chromosomal complement in human fertilized oocytes [9-15]. Nucleoli are the sites of the synthesis of pre-RNA, and ribosomal RNA (rRNA) is necessary for the translational process whereby the embryonic genome becomes fully activated [16].…”
BackgroundEmbryos selection is crucial to maintain high performance in terms of pregnancy rate, reducing the risk of multiple pregnancy during IVF. Pronuclear and nucleolar characteristics have been proposed as an indicator of embryo development and chromosomal complement in humans, providing information about embryo viability.MethodsTo correlate the zygote-score with the maternal age and the outcome of pregnancy, we analyzed the pronuclear and nucleolar morphology, the polar body alignment and the zygote configuration in 459 clinical pregnancies obtained by IVF and ICSI in our public clinic in Reggio Emilia, Italy. We derived odds ratios (OR) and Corenfield's 95% confidence intervals (CI). Continuous variables were compared with Student's t-test; P lower than .05 was considered statistically significant.ResultsWe observed a significant increase of "A" pronuclear morphology configuration in 38-41 years old patients in comparison to that lower than or equal to 32 years old and a significant decrease of "B" configuration in 38-41 years old patients in comparison to that lower than or equal to 32 and in comparison to that of 33-37 years old.Related to maternal age we found no significant differences in P1 and in P2 configuration. We found no correlation between zygote-score, embryo cleavage and embryo quality.ConclusionsOur results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality. The evaluation of embryo quality based on morphological parameters is probably more predictive than zygote-score.
“…In agreement with previously reported data [19], our analysis of nucleolar morphology showed no statistically significant differences in relation to maternal ages, both in clinical pregnancy and in ongoing pregnancy/deliveries groups. This result is in contrast with recently published data showing a correlation between pronuclear morphology and maternal ages [15]. We believe that the discrepancy could be related to the different sample size and to the different maternal age between our patients and those analyzed by Maille and coworkers.…”
Section: Discussioncontrasting
confidence: 99%
“…An important contribution in the evaluation of embryo quality seems to come from the pronuclear and nucleolar characteristics, proposed as an indicator of embryo development and chromosomal complement in human fertilized oocytes [9-15]. Nucleoli are the sites of the synthesis of pre-RNA, and ribosomal RNA (rRNA) is necessary for the translational process whereby the embryonic genome becomes fully activated [16].…”
BackgroundEmbryos selection is crucial to maintain high performance in terms of pregnancy rate, reducing the risk of multiple pregnancy during IVF. Pronuclear and nucleolar characteristics have been proposed as an indicator of embryo development and chromosomal complement in humans, providing information about embryo viability.MethodsTo correlate the zygote-score with the maternal age and the outcome of pregnancy, we analyzed the pronuclear and nucleolar morphology, the polar body alignment and the zygote configuration in 459 clinical pregnancies obtained by IVF and ICSI in our public clinic in Reggio Emilia, Italy. We derived odds ratios (OR) and Corenfield's 95% confidence intervals (CI). Continuous variables were compared with Student's t-test; P lower than .05 was considered statistically significant.ResultsWe observed a significant increase of "A" pronuclear morphology configuration in 38-41 years old patients in comparison to that lower than or equal to 32 years old and a significant decrease of "B" configuration in 38-41 years old patients in comparison to that lower than or equal to 32 and in comparison to that of 33-37 years old.Related to maternal age we found no significant differences in P1 and in P2 configuration. We found no correlation between zygote-score, embryo cleavage and embryo quality.ConclusionsOur results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality. The evaluation of embryo quality based on morphological parameters is probably more predictive than zygote-score.
“…The study of the pronuclear and nucleolar characteristics of human zygote has been proposed as an indicator of embryo development and competence [4-10]. Previous data have suggested that zygote-score could be an efficient tool for embryo selection if combined with embryo morphology evaluation on days 2 and 3 [10,11].…”
BackgroundThe assessment of the embryo quality is crucial to maintain an high pregnancy rate and to reduce the risk of multiple pregnancy. The evaluation of the pronuclear and nucleolar characteristics of human zygote have been proposed as an indicator of embryo development and chromosomal complement. The aim of the current study was to assess the role of pronuclear morphology evaluation in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles.MethodsRetrospective clinical analysis on 755 non-elective transfers of only one embryo (ET). Embryo assessment was performed in days 1 and 2. Clinical and biological data were recorded and analyzed according to embryo and/or pronuclear morphology.ResultsBoth pronuclear and embryo morphology were significantly related to clinical pregnancy and live-birth rates. No significant difference in clinical pregnancy and live-birth rates was detected when the pronuclear and embryo morphology assessments were combined. Embryo morphology and maternal age were the only independent predictors of favorable outcome by logistic regression analysis.ConclusionsPronuclear evaluation is effective to select the best zygotes if ET is performed at day 1, whereas it did not improve the clinical outcomes when combined with embryo morphology evaluation in day 2.
“…Twelve (52.2%) found a correlation [10,12,14,16,19,21,23,33],[36,39,40,43] between zygote morphology and implantation rate, while 11 studies (47.8%) did not find any correlation [15,17,26,28,30,32,34,38],[42,45,47]. …”
The current systematic review was aimed to assess the effectiveness of the zygote morphology evaluation in fresh in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles. All available studies reporting on zygote morphology and clinical and/or biological outcomes were analyzed. Forty studies were included in the final analysis. Fourteen different zygote scoring systems were employed. Zygote morphology correlated significantly with embryo quality and cleavage, blastocyst stage, embryonic chromosome status, in a high proportion of the studies which assessed the specific outcome [15/25 (60%), 15/20 (75%), 7/8 (87.5%), 6/6 (100%), respectively]. On the other hand, only a reduced proportion of papers showed a statistically significant relationship between implantation, pregnancy and delivery/live-birth rates and zygote morphology score [12/23 (52.2%), 12/25 (48%), 1/4 (25%), respectively]. In conclusion, our findings demonstrate the lack of conclusive data on the clinical efficacy of the zygote morphology evaluation in fresh IVF/ICSI cycles, even if biological results showing a good relationship with embryo viability suggest a role in cycles in which the transfer/freezing is performed at day 1.
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