Promotion of incisional wound repair by human mesenchymal stem cell transplantation

Stoff, Alexander; Rivera, Angel A.; Banerjee, N. Sanjib; Moore, Steven T.; Numnum, T. Michael; Espinosa-de-los-Monteros, Antonio; Richter, Dirk F.; Siegal, Gene P.; Chow, Louise T.; Feldman, Dale; Vásconez, Luis O.; Mathis, J. Michael; Stoff-Khalili, Mariam A.; Curiel, David T.

doi:10.1111/j.1600-0625.2008.00792.x

Cited by 116 publications

(87 citation statements)

References 36 publications

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“…No significant difference in stem cell treatment and CM treatment may be due to treatment with these two in small intervals (about 7 day interval). Similar results were reported in allogenic and xenogenic MSCs [18]. It has been reported that, surface epithelium of stem cell treated wounds became thinner and appeared more close to normal skin sections in comparison to PBS treated wound sections [19].…”

Section: Discussionsupporting

confidence: 78%

Therapeutic Potential of Canine Bone Marrow Derived Mesenchymal Stem Cells and its Conditioned Media in Diabetic Rat Wound Healing

Ansari¹,

Tr²,

Ch³

et al. 2013

J Stem Cell Res Ther

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Present study was conducted to explore the possibility of treating the diabetic rat wounds by canine bone marrow derived mesenchymal stem cells (BMSCs) and its conditioned media (CM). Canine bone marrow stem cells were isolated ascetically from dog and cultured in vitro. Stem cells conditioned media was collected from third passage cells at 120 hours of culture. Streptozotocin was used for induction of diabetes in rats. Six groups were made for the wound healing therapy in which group I, II and III were non-diabetic while group IV, V and VI were diabetic with six animals in each group and one wound was created in each animal. Group II and V received stem cells in culture media and group III and VI received conditioned media while group I and IV were kept as respective controls given only stem cell culture media. Stem cells and its conditioned media were injected at the periphery of wounds. Wound healing was assessed by wound contraction, photographic evaluation at different time interval (0, th day) and histomorphological examination on day 28. The outcomes of wound healing experiment suggested that canine bone marrow stem cells as well as its conditioned media can be exploited xenogenically very well for diabetic rat wound healing.

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Section: Discussionsupporting

confidence: 78%

Therapeutic Potential of Canine Bone Marrow Derived Mesenchymal Stem Cells and its Conditioned Media in Diabetic Rat Wound Healing

Ansari¹,

Tr²,

Ch³

et al. 2013

J Stem Cell Res Ther

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“…The papers mentioned in this study had performed such analyses in laboratory animals 39,40 , and their results, as in this research, also showed beneficial effects of cell therapy on skin healing.…”

Section: Postoperative Period -1 Month Postoperative Period -3 Monthssupporting

confidence: 55%

Adipose tissue mature stem cells in skin healing: a controlled randomized study

Martins

Uebel

Silva³

2011

Rev. Bras. Cir. Plást. (Impr.)

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ORIGINAL ARtIcLeABSTRACT Background: The differences between fetal and adult scars suggest the possibility of manipulating skin scarring outcomes. This study aimed to assess whether the use of adult stem cells from adipose tissue is beneficial to skin healing. Methods: This was a randomized controlled study for which 18 patients were selected based on inclusion and exclusion criteria. The adult stem cells used were autologous and were extracted from infraumbilical adipose tissue prior to abdominoplasty. These cells were implanted into the surgical wound dermis in the suprapubic region before skin synthesis. The results were assessed blindly based on the Draaijers scale by three physicians and by the patients themselves in a self-assessment. Photometric assessment by digital photography was also performed. Results: Among the 18 operated patients, considering the surgical result, 17 (94.4%) had excellent or good results and one (5.5%) had wound dehiscence, which was considered a bad result. Considering skin healing in the searched area, there was no statistically significant difference in the photometric evaluation; in both the self-assessment by the patients and the physicians' assessment, the results were significantly in favor of intervention with stem cells (P = 0.12 and P = 0.003, respectively). Consideration of all assessments (physicians, patients and photometric) found a statistically significant difference in favor of the implantation of adult stem cells from adipose tissue (P <0.001). Conclusions: Skin healing results after implantation of adult stem cells derived from adipose tissue were satisfactory.Keywords: Stem cells. Wound healing. Abdomen/surgery. RESUMO Introdução:Fatores que diferenciam a cicatrização fetal e a do adulto instigam a possibilidade de manipulação das soluções de continuidade da pele. Este estudo teve como objetivo avaliar se o uso de células-tronco adultas do tecido adiposo é benéfico à cicatrização da pele. Método: Estudo controlado, randomizado, para o qual foram selecionadas 18 pacientes, considerando-se critérios de inclusão e exclusão. As células-tronco adultas utilizadas eram autólogas, extraídas do tecido adiposo da região infraumbilical, precedendo a realização da abdominoplastia. Essas células, antes da síntese da pele, foram implantadas na derme da ferida operatória, na região suprapúbica. A avaliação dos resultados foi realizada com base na escala de Draaijers, por três avaliadores médicos cegados, e pelas próprias pacientes, por autoavaliação. Foi realizada, também, avaliação fotométrica por fotografia digital. Resultados: Dentre as 18 pacientes operadas, sob o ponto de vista cirúrgico, 17 (94,4%) apresentaram resultados excelentes ou bons e uma (5,5%) apresentou deiscência de sutura, considerado mau resultado. Quanto à cicatrização da pele na área pesquisada, à avaliação fotométrica, não houve diferença estatisticamente significante; à autoavaliação pelas pacientes, os resultados atingiram nível de significância a favor da intervenção com células-tronco (P = 0,12); e à...

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“…114 MSC therapy also holds promise in improving wound healing outcomes in other wound care settings, such as surgical wounds or burns. 115,116 Ongoing studies into MSC immunomodulation and wound support in other wound models (myocardial infarction, brain, long bone defects, and others) may continue support advanced wound care research with insights into novel biomaterials and beneficial properties of MSCs in cell therapy.…”

Section: Discussionmentioning

confidence: 99%