Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows

Veljović, Katarina; Dinić, Miroslav; Lukić, Jovanka; Mihajlović, Sanja; Tolinački, Maja; Živković, Milica; Begović, Jelena; Mrvaljević, Igor; Golić, Nataša; Terzić-Vidojević, Amarela

doi:10.3389/fmicb.2017.02028

Cited by 22 publications

(20 citation statements)

References 49 publications

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“…Recent studies revealed that maternal diets and gut microbiota could directly affect the offspring development early in life [32, 33, 45]. For instance, sows treated with probiotic combinations resulted in improved microbiota diversity in neonatal piglets [46]. In the current study, microbial diversity evidenced by the Shannon index showed a significant reduction in the 1.0 kg/t lysozyme-treated group (p = 0.0014, Figure 1A).…”

Section: Resultssupporting

confidence: 55%

“…Genera that exerted a significant effect on both serum immune indices and milk metabolites were filtered, and a possible regulation network is shown in Figure 6B. Based previous reports, Lactobacillus and Prevotella may play a key role in lysozyme mediated host-microbial interactions [3, 46, 50].…”

Section: Resultsmentioning

confidence: 98%

“…Previous studies reported that lysozyme could be effective against a wide range of gastrointestinal pathogens, such as Listeria monocytogenes , Clostridium perfringens , Candida spp., and Helicobacter pylori in vitro [7, 21]. Reduced microbial diversity should result from combined factors including physiological preparation for parturition and lysozyme treatment [32, 46]. Furthermore, Spirochaetes , Euryarchaeota, and Actinobacteria significantly increased while Firmicutes showed a remarkable reduction in the 1.0 kg/t treated group compared with the control group.…”

Section: Resultsmentioning

confidence: 99%

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Dietary lysozyme supplement alters serum biochemical makers and milk metabolite profile of sows via gut microbiota

Zhou

Xiong

Zou

et al. 2018

Preprint

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24Lysozyme is an important antimicrobial agent with promising future in replacing antibiotics in 25 livestok production.The aim of current study was to determine variations in sow's gut microbiota, 26 serum immunity and breast milk metabolite profile mediated by lysozyme 27 supplementation.Thirty-six pregnant sows were assigned to a control group without 28 supplementation and two treatments with 0.5 g/kg and 1.0 g/kg lysozyme provided in formula feed 29 for 21days. Microbiota analysis based on 16s RNA high-throughput sequencing and untargeted 30 liquid chromatography tandem mass spectrometry were applied and combined in analysis. Serum 31 biochemical indicators and immunoglobulins were also determined.Sows received 1.0kg/t 32 lyszoyme treatment shown significant redution in microbial diversity. Spirochaetes, Euryarchaeota 33and Actinobacteria significantly increased while Firmicutes showed a remarkable reduction in 34 1.0kg/t treated group compared with control. Pyrimidine metabolism,Purine metabolism and 35Amino acid related enzymes were significantly upregulated in 1.0kg/t lysozyme treated group. 36The richness of gram-positive bacteria were significantly down-regulated by lysozyme 37 treatments.Serum aspartate transaminase (AST) activity was significantly un-regulated.Serum IgM 38 levels were significantly higher in the 1.0 kg/t group compared with control, while IgA levels was 39 significantly lower in 1.0kg/t group.Over thirty metabolites from sow's breast milk including 40 L-Glutamine,creatine and L-Arginine were sigficantly altered by lysozyme treatment.There 41 existed crucial correlations among gut microbiota, serum immunity and breast milk metabolites 42where lactobacillus and prevotella may play a key role in lysozyme mediated host-microbial 43 interactions. Overall, lysozyme supplementation could effectively improve the composition, 44 metabolic functions and phenotypes of sow's gut microbiota and it also benefit sows with better 45 immune status and breast milk composition. 46 47 Importance: Enteric infections caused by pathogens have a significant negative effect on neonatal 48 survival and animal health in swine production. The application of antibiotics in feeds at 49 subtherapeutic levels could improve performance and overall health and is used extensively 50 throughout the industry. However, abuse of antibiotics is contributing to the high level of drug 51 resistance in microbial communities and rising concerns regarding human health. Here, we 52 revealed that lysozyme supplementation could effectively improve the composition, metabolic 53 functions and phenotypes of sow's gut microbiota and it also benefit sows with better immune 54 status and breast milk composition. These findings confirmed that lysozyme could be a suitable 55 alternative to antibiotics in swine production. 56 57 58 59 60 lysozyme alters sow's gut microbiota and health 61 62 63 64 65 66 67

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Section: Resultssupporting

confidence: 55%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Dietary lysozyme supplement alters serum biochemical makers and milk metabolite profile of sows via gut microbiota

Zhou

Xiong

Zou

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Sows treated with probiotic combinations resulted in improved microbiota diversity in neonatal piglets (Veljovic et al, 2017). In the current study, microbial diversity evidenced by the Shannon index showed a significant reduction in the 1.0 kg/t lysozyme-treated group ( p = 0.0014, Figure 1A).…”

Section: Discussionmentioning

confidence: 99%

Dietary Lysozyme Alters Sow’s Gut Microbiota, Serum Immunity and Milk Metabolite Profile

et al. 2019

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The aim of current study was to determine variations in sow’s gut microbiota, serum immunity, and milk metabolite profile mediated by lysozyme supplementation. Twenty-four pregnant sows were assigned to a control group without supplementation and two treatments with 0.5 kg/t and 1.0 kg/t lysozyme provided in formula feed for 21 days (n = 8 per treatment). Microbiota analysis and metagenomic predictions were based on 16s RNA high-throughput sequencing. Milk metabolome was assessed by untargeted liquid chromatography tandem mass spectrometry. Serum biochemical indicators and immunoglobulins were also determined. Gut microbial diversity of sows receiving 1.0 kg/t lysozyme treatment was significantly reduced after the trial. Spirochaetes, Euryarchaeota, and Actinobacteria significantly increased while Firmicutes showed a remarkable reduction in 1.0 kg/t group compared with control. Lysozyme addition rebuilt sow’s gut microbiota to beneficial composition identified by reduced richness of Escherichia coli and increased abundance of Lactobacillus amylovorus. Accordingly, microbial metabolic functions including pyrimidine metabolism, purine metabolism, and amino acid related enzymes were significantly up-regulated in 1.0 kg/t group. Microbial metabolic phenotypes like the richness of Gram-positive bacteria and oxidative stress tolerance were also significantly reduced by lysozyme treatment. Serum alanine transaminase (ALT) activity and IgA levels were significantly down-regulated in the 1.0 kg/t group compared with control, but IgM levels showed a significantly increase in 1.0 kg/t group. Milk metabolites such as L-glutamine, creatine, and L-arginine showed significantly dose-dependent changes after treatment. Overall, lysozyme supplementation could effectively improve the composition, metabolic functions, and phenotypes of sow’s gut microbiota and it also benefit sows with better serum immunity and milk composition. This research could provide theoretical support for further application of lysozyme in promoting animal gut health and prevent pathogenic infections in livestock production.

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“…belong to the physiological gut flora of suckling piglets and have anti-inflammatory properties within the gut microbial community (5), any potential positive effect might have been superimposed by the pathogenic effects of infection with C. suis . In general, the health-promoting effects of probiotic bacteria depend on neutralization of effects of pathogens by various mechanisms, as blocking of colonization sites, reinforcement of tight-junctions, mucus induction, and stimulation of innate immune responses (42, 43). The intestinal development of C. suis starts with excystation of sporozoites in the gut lumen and their intrusion into enterocytes (44).…”

Section: Discussionmentioning

confidence: 99%

Treatment With Probiotic Bacteria Does Not Diminish the Impact of a Cystoisospora suis Challenge in Suckling Piglets

et al. 2018

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Colonization of newborn piglets with beneficial and ubiquitous microorganisms in combination with colostral passive immunity is the prerequisite for development of immunity and gut maturation. In this study living strains of Clostridium perfringens type A (CpA) and non-pathogenic Escherichia (E.) coli strains harvested from healthy piglets were administered to piglets prior to first colostrum intake in order to prevent disease caused by pathogenic variants of the same bacterial species by competitive exclusion. In addition, it was investigated whether these potential beneficial colonizers were able to prevent harmful effects of infection with Cystoisospora (C.) suis as a primary invasive pathogen. In a first trial, half of the piglets from four litters were treated with a bacterial cocktail consisting of two E. coli and four CpA strains immediately after birth on two consecutive days, while the other half of the litters served as control group. In a second trial, piglets were treated following the protocol of the first trial, and additionally all piglets were infected 4 h after the end of littering with ~1,000 sporulated oocysts of a C. suis laboratory strain. General health, body weight development, fecal consistency and, in the second trial, oocyst excretion were monitored from birth until weaning. No adverse effects of the cocktail on the health status were observed. Treated piglets of the first trial showed a higher average daily weight gain until weaning. In the second trial, no significant differences were found with respect to average daily weight gain, fecal consistency, amount, and duration of oocyst excretion assessed in daily samples. In treatment group 51.1% and in the control group 38.5% of the fecal samples were positive for oocysts in autofluorescence. The average duration of oocyst excretion was longer in treatment group (7.7 days) than in control group (5.6 days). Application of bacterial cocktail could not effectively minimize disease symptoms caused by C. suis. There was a trend toward an increase in severity of disease symptoms in treated pigs, suggesting that the synergism between CpA and C. suis was independent of the bacterial strains, but is exclusively dominated by the pathogenic effect of C. suis.

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Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows

Cited by 22 publications

References 49 publications

Dietary lysozyme supplement alters serum biochemical makers and milk metabolite profile of sows via gut microbiota

Dietary lysozyme supplement alters serum biochemical makers and milk metabolite profile of sows via gut microbiota

Dietary Lysozyme Alters Sow’s Gut Microbiota, Serum Immunity and Milk Metabolite Profile

Treatment With Probiotic Bacteria Does Not Diminish the Impact of a Cystoisospora suis Challenge in Suckling Piglets

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