Dietary Lysozyme Alters Sow’s Gut Microbiota, Serum Immunity and Milk Metabolite Profile

Zhou, Jian; Xiong, Xia; Yin, Jia; Zou, Lijun; Wang, Kexing; Shao, Yirui; Yin, Yulong

doi:10.3389/fmicb.2019.00177

Cited by 22 publications

(23 citation statements)

References 64 publications

(106 reference statements)

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“…A heat map of the 40 most different genera showed the taxonomic distributions among groups after 3 and 6 weeks of treatment (Supplementary Figure S1). Next, the impact of Cu treatment on aerobic, anaerobic, mobile element-containing, facultatively anaerobic, biofilm-forming, Gram-negative, Grampositive, potentially pathogenic, and stress-tolerant bacteria were predicted via BugBase (Zhou et al, 2019). No significant differences were found to occur as a result of Cu treatment (Supplementary Figure S3).…”

Section: Microbiota Composition Of Intestinal Contentsmentioning

confidence: 99%

Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles of Escherichia coli in Weaned Piglets

et al. 2019

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Copper is an essential microelement for animals, and not only it has been used as a feed additive at pharmacological doses in swine production to improve growth performance, but it also has an effect on intestinal microbes by enhancing host bacterial resistance. However, there are few reports on the effects of pharmacological doses of copper on intestinal microorganisms and the antimicrobial resistance profiles of pathogenic bacteria, such as Escherichia coli, in pigs. Therefore, this study aimed to investigate the effects of pharmacological doses of copper on the microbial communities in the hindgut and the antimicrobial resistance profiles of E. coli in weaned piglets. Twenty-four healthy weaned piglets aged 21 ± 1 days and with an average weight of 7.27 ± 0.46 kg were randomly divided into four groups. The control group was fed a basal diet, while the treatment groups were fed a basal diet supplemented with 20, 100, or 200 mg copper/kg feed, in the form of CuSO 4. Anal swabs were collected at 0, 21, and 42 days of the trial, and E. coli was isolated. Meanwhile, the contents of the ileum and cecum from the control and 200 mg copper/kg feed groups were collected at 21 and 42 days for microbial community analysis and E. coli isolation. All isolated E. coli strains were used for antimicrobial resistance profile analysis. A pharmacological dose of copper did not significantly change the diversity, but significantly affected the composition, of microbial communities in the ileum and cecum. Moreover, it affected the microbial metabolic functions of energy metabolism, protein metabolism, and amino acid biosynthesis. Specifically, copper treatment increased the richness of E. coli in the hindgut and the rates of E. coli resistance to chloramphenicol and ciprofloxacin. Moreover, the rate of E. coli resistance to multiple drugs increased in the ileum of pigs

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Section: Microbiota Composition Of Intestinal Contentsmentioning

confidence: 99%

Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles of Escherichia coli in Weaned Piglets

et al. 2019

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“…All experiments were approved by the Animal Care and Use Committee of the Institute of Subtropical Agriculture, Chinese Academy of Sciences 25 . Thirty-six male ICR mice (three-week-old) were obtained from the SLAC Laboratory Animal Central (Changsha, China).…”

Section: Animals and Experimental Designmentioning

confidence: 99%

“…Serum levels of total bile acid(TBA) were detected by the fully automatic biochemical analyzer(Shenzhen Mindray, BS-190), as described in our previous studies 25 . Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and dual amine oxidase(DAO) were determined via commercial kits, and all determinations were done in triplicate and performed according to the manufacturer's instructions 25 .…”

Section: Serum Biochemical Analysismentioning

confidence: 99%

“…Raw 16S data sequences The first-strand before being screened and assembled using the QIIME (v1.9.1) and FLASH software packages as previously described 25 13 . Functional metagenomes of all samples were predicted using Tax4Fun R packages(http://tax4fun.gobics.de) 31 .…”

Section: Bioinformatic Analysis and Metagenomic Predictionsmentioning

confidence: 99%

“…Tight junction proteins like occludin and ZO1 also contribute to the regulation of intestinal permeability that physically against the invasion of gut bacteria 37 . Antimicrobial proteins like BD1, BD2, and Reg3 family are often secreted by Paneth cells to clear up the pathogens and promote epithelial regeneration following intestinal infection 2,23,25 . However, IBD patients often suffered from prolonged enteric inflammation that ultimately damages intestinal epithelia, and subsequently leads to increased intestinal permeability that causing pathogenic infections 35 .…”

Section: Ethanolamine Promoted Tlr4/myd88 Dependent Signaling In Inflmentioning

confidence: 99%

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Ethanolamine improves colonic barrier functions and inflammatory immunoreactions via shifting microbiome dysbiosis

Zhou

Xiong

Wan

et al. 2020

Preprint

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Ethanolamine(EA) often occurs at a relatively high concentration within the inflamed gut of IBD patients. To investigate the role of EA in colonic inflammation and host-microbiome dysbiosis, thirty-six ICR mice were treated with 3% DSS for a week to generate acute intestinal inflammation and then supplied with 0 μM, 500 μM (LowEA), and 3000 μM (HighEA) in drinking water for two weeks, after that, 16s RNA sequencing was applied in characterizing the changes in colonic microbiota driven by different EA levels. An inflamed colonic organoid model via 3% DSS treatment was also established for further verification of these in vivo findings. EA significantly reduced proximal colonic crypt depth but increased distal colonic villus height in HighEA group. The protein and mRNA expression of occludin and Reg3β, BD1, BD2, and MUC2 were significantly up-regulated in EA treated groups. EA decreased mucosal inflammation-related cytokines levels (IL1, IL6, IL17, TNFα, and INFγ) and increased the significantly increased concentration of sIgA. Serum aspartate aminotransferase and alanine aminotransferase were significantly down-regulated in the highEA group. EA increased the relative abundance of Blautia, Roseburia, Lactobacillus, Faecalibaculum, Candidatus_Saccharimonas, Alloprevotella, and Lachnoclostridum and thus microbial metabolic pathways including Oxidative phosphorylation, Lipopolysaccharide biosynthesis, Arginine and proline metabolism, Folate biosynthesis, and Biotin metabolism were more abundant in LowEA group than those in control. EA up-regulated the protein or mRNA expression of TLR4/MyD88 in colonic tissues and the DSS-treated colonic organoid model. This study firstly demonstrated that ethanolamine in altering host-microbiome dysbiosis, which may provide new insights into the role of dietary lipids in IBD.

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Effects of supplementing sow diets during late gestation with Pennisetum purpureum on antioxidant indices, immune parameters and faecal microbiota

Huang

Mou

Yang

et al. 2021

Veterinary Medicine & Sci

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The purpose of this study was to investigate the effects of adding Pennisetum purpureum (P. purpureum, also known as Napier grass or elephant grass) to the diets of late gestation on the antioxidant indexes, immune indexes and faecal microbiota of sows. At the 90 days of gestation, 300 healthy sows were randomly divided into three groups, and they received the basic commercial diet or added 5% P. purpureum and 10% P. purpureum, respectively. The experiment started from 90 days of gestation to parturition. The results showed that the total antioxidant capacity, immunoglobulins and serum equol concentrations of sows on 100 days of gestation and at parturition increased linearly (p < .05) with the increase of the content of P. purpureum in the gestation diet. The 5% P. purpureum increased the relative abundance of Bacteroidetes (p = .027) and Actinobacteria (p < .001) at phylum level, Coriobacteriaceae (p < .001) at family level and Prevotellaceae_UCG_001 (p = .004) at genus level, and decreased the relative abundance of Escherichia_Shigella (p < .001) at genus level. In summary, this study shows that the additive of P. purpureum can increase the concentration of serum equol, improve the antioxidant capacity and immune function of sow in late gestation. In addition, the additive of 5% P. purpureum in the diet might change the composition of intestinal microbiota of sows, particularly the relative abundance of Coriobacteriaceae (p < .001) increased.

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Dietary Lysozyme Alters Sow’s Gut Microbiota, Serum Immunity and Milk Metabolite Profile

Cited by 22 publications

References 64 publications

Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles of Escherichia coli in Weaned Piglets

Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles of Escherichia coli in Weaned Piglets

Ethanolamine improves colonic barrier functions and inflammatory immunoreactions via shifting microbiome dysbiosis

Effects of supplementing sow diets during late gestation with Pennisetum purpureum on antioxidant indices, immune parameters and faecal microbiota

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