Promotion of Burn Wound Healing by Local Application of Adipose-Derived Mesenchymal Stem Cells: An Experimental Study

Yazdi, Farzane Rezaei; Ghahary, Aziz; Mirdoraghi, Mohammad; Sarvnaz, Hamzeh; Asgardoon, Mohammad Hossein; Rastegar, Tayebeh; Malek, Fatemeh; Moayyer, Tahereh Abbasi; Dafchahi, Kamyar Ghaffari; Takzaree, Nasrin

doi:10.47176/mjiri.35.172

Cited by 2 publications

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“…ADSCs promoted VEGF gene expression and secretion of TGF-β. ADSC also promoted the proliferation of dermal fibroblasts [ 50 ]. Injected intradermally, allogenic ADSCs reduced the area of the burn after 14 days as well as reduced lymphatic vessels and influenced the concentration of collagen type III [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

Mesenchymal Stem Cells in Burn Wound Management

Surowiecka

Chrapusta²,

Klimeczek-Chrapusta³

et al. 2022

IJMS

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Mesenchymal stem cells have a known regenerative potential and are used in many indications. They secrete many growth factors, including for fibroblasts (FGF), endothelium (VEGF), as well as 14 anti-inflammatory cytokines, and they stimulate tissue regeneration, promoting the secretion of proteins and glycosaminoglycans of extracellular matrices, such as collagen I, II, III, and V, elastin, and also metalloproteinases. They secrete exosomes that contain proteins, nucleic acids, lipids, and enzymes. In addition, they show the activity of inactivating free radicals. The aim of this study was an attempt to collect the existing literature on the use of stem cells in the treatment of a burn wound. There were 81 studies included in the analysis. The studies differed in terms of the design, burn wound model, source of stem cells, and methods of cellular therapy application. No major side effects were reported, and cellular therapy reduced the healing time of the burn wound. Few case reports on human models did not report any serious adverse events. However, due to the heterogeneity of the evidence, cellular therapy in burn wound treatment remains an experimental method.

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Section: Methodsmentioning

confidence: 99%

Mesenchymal Stem Cells in Burn Wound Management

Surowiecka

Chrapusta²,

Klimeczek-Chrapusta³

et al. 2022

IJMS

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show abstract

“…A significant aspect of such techniques is the use of mesenchymal stem cells (hereinafter referred to as MSCs). According to recent studies, MSCs demonstrate high effectiveness in wound healing, not only due to their proliferative activity but also to their paracrine immunomodulatory effects and stimulation of the reparative processes ( 9 - 11 ).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness and Safety of Transplantation of the Stromal Vascular Fraction of Autologous Adipose Tissue for Wound Healing in the Donor Site in Patients with Third-Degree Skin Burns: A Randomized Trial

Ryabkov,

Egorikhina,

Koloshein

et al. 2023

MJIRI

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Background The quality of the wound healing at the donor site significantly determines the overall condition of the burn patient, the extent of wound fluid and protein losses, the severity of any systemic in-flammatory reaction, and the intensity of the pain syndrome. It is known that the stromal vas-cular fraction (SVF) has a beneficial effect on the healing of wound defects. This study is aimed at assessing the safety and effectiveness of the application of the SVF of autologous adipose tis-sue to stimulate wound healing of the donor site in patients with burns. Methods This placebo-controlled clinical study included 38 patients with third-degree thermal skin burns. The patients underwent liposuction, enzymatic isolation of the SVF, and intradermal injection of the preparation into the wounds in the donor site, followed by tewametry, cutome-try, thermography and biopsy after 12 days. Quantitative indicators were compared using the Mann-Whitney test for unrelated groups and the Wilcoxon test for related groups. Spearman's rank correlation coefficient (RS) was used to assess the correlation Results Epithelization of the wounds in all patients was seen over an average area of 88 (84;92) %, there being no significant differences between the actual and the control wound sites for this parameter. Transdermal water loss in the test wound sites was 2 times lower than in the control sites ( P = 0.001). The wound donor sites regained their temperature distribution faster than the control sites ( P = 0.042). Histological preparations of the skin of the wound sites revealed that their epidermal layer was 19% thicker compared to the controls ( P = 0.043). It should be noted that five adverse events related to manipulations in the postoperative period were registered. Conclusion Transplantation of SVF autologous adipose tissue into the wound area in most clinical cases proceeded without complications. The area of epithelialization of wound areas af-ter the introduction of SVF did not change, although a significant decrease in transdermal water loss was observed in the wound areas with an improvement in their thermoregulation and an increase in the thickness of the epidermis.

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Promotion of Burn Wound Healing by Local Application of Adipose-Derived Mesenchymal Stem Cells: An Experimental Study

Cited by 2 publications

References 0 publications

Mesenchymal Stem Cells in Burn Wound Management

Mesenchymal Stem Cells in Burn Wound Management

Effectiveness and Safety of Transplantation of the Stromal Vascular Fraction of Autologous Adipose Tissue for Wound Healing in the Donor Site in Patients with Third-Degree Skin Burns: A Randomized Trial

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