Promotion of Angiogenesis by Low Energy Laser Irradiation

Mirsky, Nitsa; Krispel, Y.; Shoshany, Yariv; Maltz, Lidya; Oron, Uri

doi:10.1089/152308602760598936

Cited by 58 publications

(36 citation statements)

References 26 publications

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“…The effect of LELI on the formation process of new blood vessels in the infarcted rat heart monitored by counting the proliferation of EC in blood vessels has been established in a recent study [61]. It also seemed that laser therapy stimulated lymphocytes to produce factors that could modulate EC proliferation in vitro [62].…”

Section: Discussionmentioning

confidence: 99%

Low‐energy laser irradiation increases endothelial cell proliferation, migration, and eNOS gene expression possibly via PI3K signal pathway

2008

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Background and Objectives: The purpose of this study, therefore, was to determine the mechanisms by which lowenergy laser irradiation (LELI) may exert some of its angiogenic effects via the PI3 kinase/eNOS signaling pathway and induce endothelial cell migration and neovascularization, an important and necessary part of wound healing. Study Design/Materials and Methods: The possible molecular mechanism of helium-neon (He-Ne) laser irradiation on endothelial cells was proposed. He-Ne laser at 632.5 nm was used to stimulate human umbilical vein endothelial cell (HUVEC), and its effect on cell proliferation, nitric oxide secretion, and cell migration was determined. Results: Irradiation enhanced endothelial nitric oxidase synthase (eNOS) protein expression, and irradiation of less than 0.26 J/cm 2 enhanced eNOS gene expression in HUVEC. The cell migration ability was promoted for HUVEC irradiated with 0.26 J/cm 2 . This agreed with the vinculin protein expression induced by irradiation. In addition, the angiogenesis was promoted. The induced eNOS expression was inhibited by LY294002, indicating that the effect of laser on EC could be attributed to the upregulation of eNOS expression through PI3K pathway at the cellular and molecular levels as a result of the He-Ne laser. Conclusions: The study has shown that LELI increased endothelial cell proliferation, migration, NO secretion, and identified that activation of PI3K/Akt pathway was a critical step for the elevated for eNOS expression upon LELI.

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Section: Discussionmentioning

confidence: 99%

Low‐energy laser irradiation increases endothelial cell proliferation, migration, and eNOS gene expression possibly via PI3K signal pathway

2008

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“…[40][41][42] Light can penetrate several tissues, including both the scalp and skull, and reach into the brain. Several clinical and preclinical studies have shown that this process can lead to an improved recovery from stroke.…”

Section: Mechanisms Of Tllltmentioning

confidence: 99%

Transcranial Low-Level Laser (Light) Therapy for Brain Injury

Thunshelle

Hamblin

2016

Photomedicine and Laser Surgery

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Background: Low-level laser therapy (LLLT) or photobiomodulation (PBM) is a possible treatment for brain injury, including traumatic brain injury (TBI). Methods: We review the fundamental mechanisms at the cellular and molecular level and the effects on the brain are discussed. There are several contributing processes that have been proposed to lead to the beneficial effects of PBM in treating TBI such as stimulation of neurogenesis, a decrease in inflammation, and neuroprotection. Both animal and clinical trials for ischemic stroke are outlined. A number of articles have shown how transcranial LLLT (tLLLT) is effective at increasing memory, learning, and the overall neurological performance in rodent models with TBI. Results: Our laboratory has conducted three different studies on the effects of tLLLT on mice with TBI. The first studied pulsed against continuous laser irradiation, finding that 10 Hz pulsed was the best. The second compared four different wavelengths, discovering only 660 and 810 nm to have any effectiveness, whereas 732 and 980 nm did not. The third looked at varying regimens of daily laser treatments (1, 3, and 14 days) and found that 14 laser applications was excessive. We also review several studies of the effects of tLLLT on neuroprogenitor cells, brain-derived neurotrophic factor and synaptogenesis, immediate early response knockout mice, and tLLLT in combination therapy with metabolic inhibitors. Conclusions: Finally, some clinical studies in TBI patients are covered.

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“…10,[35][36][37][38][39] It has been reported that angiogenesis can cause transiently leaky microvessels 35,39 ; therefore, we wanted to determine the effect of TLT alone and in combination with tPA treatment, which is known to cause vascular damage and hemorrhage. We were concerned that TLT might exacerbate thrombolytic-induced ICH.…”

Section: Figurementioning

confidence: 99%

Safety Profile of Transcranial Near-Infrared Laser Therapy Administered in Combination With Thrombolytic Therapy to Embolized Rabbits

Lapchak

Han

Salgado

et al. 2008

Stroke

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Background and Purpose-Transcranial near-infrared laser therapy (TLT) is currently under investigation in a pivotal clinical trial that excludes thrombolytic therapy. To determine if combining tissue plasminogen activator (tPA; Alteplase) and TLT is safe, this study assessed the safety profile of TLT administered alone and in combination with Alteplase. The purpose for this study is to determine if the combination of TLT and thrombolysis should be investigated further in a human clinical trial. Methods-We determined whether postembolization treatment with TLT in the absence or presence of tPA would affect measures of hemorrhage or survival after large clot embolism-induced strokes in New Zealand white rabbits. Results-TLT did not significantly alter hemorrhage incidence after embolization, but there was a trend for a modest reduction of hemorrhage volume (by 65%) in the TLT-treated group compared with controls. Intravenous administration of tPA, using an optimized dosing regimen, significantly increased hemorrhage incidence by 160%. The tPA-induced increase in hemorrhage incidence was not significantly affected by TLT, although there was a 30% decrease in hemorrhage incidence in combination-treated rabbits. There was no effect of TLT on hemorrhage volume measured in tPA-treated rabbits and no effect of any treatment on 24-hour survival rate. Conclusion-In the embolism model, TLT administration did not affect the tPA-induced increase in hemorrhage incidence.TLT may be administered safely either alone or in combination with tPA because neither treatment affected hemorrhage incidence or volume. Our results support the study of TLT in combination with Alteplase in patients with stroke.

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Promotion of Angiogenesis by Low Energy Laser Irradiation

Cited by 58 publications

References 26 publications

Low‐energy laser irradiation increases endothelial cell proliferation, migration, and eNOS gene expression possibly via PI3K signal pathway

Low‐energy laser irradiation increases endothelial cell proliferation, migration, and eNOS gene expression possibly via PI3K signal pathway

Transcranial Low-Level Laser (Light) Therapy for Brain Injury

Safety Profile of Transcranial Near-Infrared Laser Therapy Administered in Combination With Thrombolytic Therapy to Embolized Rabbits

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