Low‐energy laser irradiation increases endothelial cell proliferation, migration, and eNOS gene expression possibly via PI3K signal pathway

Chen, Chung Huang; Hung, Hsin-Chia; Hsu, Shan‐hui

doi:10.1002/lsm.20589

Cited by 125 publications

(104 citation statements)

References 62 publications

(59 reference statements)

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“…In addition to vasorelaxation, NO also regulates endothelial cell growth, migration, and apoptosis [17,18]. Consistent with results of the present study, a previous report also indicated that low-energy laser irradiation increased eNOS activity through phosphorylation of PI3K/Akt in human umbilical vein endothelial cells (HUVECs) [19].…”

Section: Irradiation With a 445 Nm Laser Contracted Aortic Rings By Asupporting

confidence: 92%

Diverse effects of a 445 nm diode laser on isometric contraction of the rat aorta

Park¹,

Shin²,

Park³

et al. 2015

Biomed. Opt. Express

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Abstract:The usefulness of visible lasers in treating vascular diseases is controversial. It is probable that multiple effects of visible lasers on blood vessels and their unclear mechanisms have hampered the usefulness of this therapy. Therefore, elucidating the precise actions and mechanisms of the effects of lasers on blood vessels would provide insight into potential biomedical applications. Here, using organ chamber isometric contraction measurements, western blotting, patch-clamp, and en face immunohistochemistry, we showed that a 445 nm diode laser contracted rat aortic rings, both by activating endothelial nitric oxide synthase and by increasing oxidative stress. In addition to the effects on the endothelium, the laser also directly relaxed and contracted vascular smooth muscle by inhibiting L-type Ca 2+ channels and by activating protein tyrosine kinases, respectively. Thus, we conclude that exposure to 445 nm laser might contract and dilate blood vessels in the endothelium and smooth muscle via distinct mechanisms.

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Section: Irradiation With a 445 Nm Laser Contracted Aortic Rings By Asupporting

confidence: 92%

Diverse effects of a 445 nm diode laser on isometric contraction of the rat aorta

Park¹,

Shin²,

Park³

et al. 2015

Biomed. Opt. Express

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“…13,14,15,16,17,18,19 It is important to state that the increase of cell viability is a major issue for stem cells, primarily when the objective of stem cell culture is to implant them into human organs during cellular therapy. Several in vitro studies have already explored the effects of LPT on mesenchymal stem cells (derived from bone marrow and fat tissue).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the cells with a lower pH than normal are more sensitive to LPT than those with parameters being optimal or close to it. 12,13,17,18,19,20 In this study, we used two different energy densities to analyze the effects of LPT on stem cells from deciduous teeth. Both protocols (i.e., 3 J/cm2 and 5 J/cm2) presented with significantly higher values of cellular proliferation compared with the negative control group (5% FBS).…”

mentioning

confidence: 99%

Low-intensity laser phototherapy enhances the proliferation of dental pulp stem cells under nutritional deficiency

et al. 2016

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Dental trauma in immature permanent teeth can damage pulp vascularization, which leads to necrosis and cessation of apexogenesis. Studies on tissue engineering using stem cells from human exfoliated deciduous teeth (SHEDs) have yielded promising results. Laser phototherapy (LPT) is able to influence the proliferation and differentiation of these cells, which could improve tissue engineering. SHEDs (eighth passage) were seeded into 96-well culture plates (10 3 cells/well) and were grown in culture medium supplemented with 15% defined fetal bovine serum (FBS) for 12 h. After determining the appropriate nutrition deficiency status (5% FBS), the cells were assigned into four groups: 1) G1 -15% FBS (positive control); 2) G2 -5% FBS (negative control); 3) G3 -5% FBS+LPT 3 J/cm 2 ; and 4) G4 -5% FBS+LPT 5 J/cm 2 . For the LPT groups, two laser irradiations at 6 h intervals were performed using a continuous wave InGaAlP diode laser (660 nm, with a spot size of 0.028 cm 2 , 10 mW) in punctual and contact mode. Cell viability was assessed via an MTT reduction assay immediately after the second laser irradiation (0 h) and 24, 48, and 72 h later. We found that G3 and G4 presented a significantly higher cell growth rate when compared with G2 (p < 0.01). Moreover, G4 exhibited a similar cell growth rate as G1 throughout the entire experiment (p > 0.05). These findings indicate that LPT with 5 J/cm 2 can enhance the growth of SHEDs during situations of nutritional deficiency. Therefore, LPT could be a valuable adjunct treatment in tissue engineering when using stem cells derived from the dental pulp of primary teeth.

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“…However, the molecular mechanisms underlying the biological effects induced by LLLI have not been thoroughly elucidated. Previous studies 14,15 show that LLLI regulates different biological processes and ultimately affects cell physiology by using different signaling pathways in different cell types. Previous studies also show that red light to near-infrared light is absorbed by the mitochondrial respiratory chain and the irradiation treatment led to increased production of reactive oxygen species (ROS), nitric oxide (NO), adenosine triphosphate (ATP), and cyclic adenosine monophosphate (cAMP).…”

Section: Introductionmentioning

confidence: 99%

Biological effects of low-level laser irradiation on umbilical cord mesenchymal stem cells

Chen

Wang

et al. 2016

AIP Advances

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Low-level laser irradiation (LLLI) can enhance stem cell (SC) activity by increasing migration and proliferation. This study investigated the effects of LLLI on proliferation, enzymatic activity, and growth factor production in human umbilical cord mesenchymal SCs (hUC-MSCs) as well as the underlying mechanisms. hUC-MSCs were assigned to a control group (non-irradiation group) and three LLLI treatment groups (635 nm group, 808 nm group, and 635/808 nm group). Laser power density and energy density of 20 mW/cm2 and 12 J/cm2, respectively, were used for each experiment. The proliferation rate was higher in the 635 nm as compared to the other groups. LLLI at 808 nm did not induce cell proliferation. ROS levels in cells exposed to 635, 808, and 635/808 nm radiation were increased by 52.81%, 26.89%, and 21.15%, respectively, relative to the control group. CAT, tGPx, and SOD activity was increased. LLLI at 808 nm increased the levels of IL-1, IL-6, and NFκB but not VEGF. LLLI improved hUC-MSCs function and increased antioxidant activity. Dual-wavelength LLLI had more potent effects on hUC-MSCs than single-wavelength treatment. LLLI has potential applications in the preconditioning of hUC-MSCs in vitro prior to transplantation, which could improve the regenerative capacity of cells.

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Low‐energy laser irradiation increases endothelial cell proliferation, migration, and eNOS gene expression possibly via PI3K signal pathway

Cited by 125 publications

References 62 publications

Diverse effects of a 445 nm diode laser on isometric contraction of the rat aorta

Diverse effects of a 445 nm diode laser on isometric contraction of the rat aorta

Low-intensity laser phototherapy enhances the proliferation of dental pulp stem cells under nutritional deficiency

Biological effects of low-level laser irradiation on umbilical cord mesenchymal stem cells

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