Promoting Tissue Repair by Micrograft Stem Cells Delivery

Trovato, Letizia; Naro, Fabio; D’Aiuto, F; Moreno, Federico

doi:10.1155/2020/2195318

Cited by 10 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7 Skin MG produced using the Rigenera protocol contains all skin tissue components, including tissue stem cells, progenitor cells, and various tissue factors. 22 In particular, mesenchymal stem cells as multipotent cells have trophic and support functions, releasing anti-inflammatory cytokines and anti-apoptotic molecules to promote protection and/or repair of damaged tissues. [23][24][25] In addition, the extracellular components of MG contain cytokines, growth factors, proteinases, and matrix components, which are important candidates as paracrine factors for wound healing.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Wound Healing Effect of Skin Micrograft Impregnated into Two Kinds of Artificial Dermis in a Murine Wound Model

Sakamoto

Sawaragi

et al. 2022

Plastic and Reconstructive Surgery - Global Open

View full text Add to dashboard Cite

Background: A micrograft (MG) suspension produced by the Rigenera protocol has been used to stimulate tissue regeneration. Recently, a combination therapy of an artificial dermis and skin MG has been used to promote angiogenesis and granulation tissue formation in the artificial dermis. There are no reports comparing the differences in MG impregnation efficiency between different artificial dermis products. Therefore, we compared the impregnation of skin MG in Pelnac Gplus and Integra. Methods: Skin MG was prepared from the skin of C57BL/6JJcl mice using Rigeneracons and administered onto Pelnac Gplus and Integra sheets. The amount of MG suspension impregnated in Pelnac Gplus and Integra was evaluated. Pelnac Gplus and Integra sheets combined with MG were applied to murine defects, and wound area, neoepithelium length, granulation tissue formation, and newly formed capillaries were compared with the control groups on days 7 and 14. Results: The weight percentage of the MG absorbed by Pelnac Gplus and Integra was 88.8% ± 3.5% and 28.2% ± 7.0%, respectively (P < 0.05). In the in vivo experiment, the area of newly formed granulation tissue and both the number and area of newly formed capillaries in the PelnacG + MG group were significantly larger than those in the control group at 14 days after implantation (P < 0.05). Conclusions: Skin MG was successfully impregnated into Pelnac Gplus by simple administration but not into Integra. Administration of skin MG into the Pelnac Gplus promoted granulation formation and angiogenesis. Pelnac Gplus was more suitable than Integra in the combination therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of Wound Healing Effect of Skin Micrograft Impregnated into Two Kinds of Artificial Dermis in a Murine Wound Model

Sakamoto

Sawaragi

et al. 2022

Plastic and Reconstructive Surgery - Global Open

View full text Add to dashboard Cite

show abstract

“…Several articles have suggested the use of cell cultures in periodontal regeneration but their high cost and the need to involve specialist laboratories remain a crucial problem for dentists. Nevertheless, according to Trovato et al, the use of a micrograft (a collagen membrane enriched with stem cells) has potential in the regenerative process [74]. A recent review by Mummolo et al suggested that micrografts in periodontal and bone regeneration are a good alternative to GTR or to other biomaterials such as bone substitutes [75].…”

Section: Future Therapiesmentioning

confidence: 99%

Periodontal Regeneration

2021

View full text Add to dashboard Cite

show abstract

“…Several studies have shown that stem cells progeny divide rapidly; however, the turnover term of these cells in the skin and bone marrow is relatively slow (Liu et al, 2016). Also, evidence suggests that stem cells are involved in repairing organs and tissues following injury (Trovato et al, 2020;Nourian Dehkordi et al, 2019). Research has characterized adult stem cells and their niches in different organs such as the bone marrow, skin, liver, intestine, gastrointestinal mucosa, brain and prostate, and there is reliable evidence of the presence of such cells in the kidney (Liu et al, 2016;Liu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Isolation of adult rat kidney derived stem cells and differentiation into podocyte-like cells

Mansouri¹,

Gorji²,

Absalan³

2022

EJA

View full text Add to dashboard Cite

Studies have shown that adult stem cells can be isolated from different organs. However, no agreement has yet been reached on the identification of adult kidney stem cells. In this study, we have shown that the adult rat kidney contains a population of stem cells that are isolatable and, under suitable culture conditions, these stem cells can differentiate into podocyte. Six adult Wistar rats were used in the present study. Their kidneys were chopped and exposed to collagenase I. Then, with passing through 100 μm mesh, single cells in filtered fraction were cultured in a proliferation medium. After 4 passages, the cells were analyzed by flow cytometry, differentiation and gene expression. Morphological analyses revealed that isolated cells are spindle-shaped, and can differentiate into osteocytes and adipocytes. Flow cytometry demonstrated that these cells expressed cell surface markers CD44, CD90, CD133, c-kit, Pax- 2, Oct4, sca-1 and vimentin significantly. Also, the high expression of Wt-1 and Wnt-4 genes was seen in these cells. Moreover, expression of synaptopodin and podocalyxin genes showed differentiation of stem cells into podocyte cells. Our findings demonstrate that isolated cells from the kidney of adult rats are mesenchymal stem cells or progenitor cells and can differentiate to podocyte-like cells.

show abstract

Promoting Tissue Repair by Micrograft Stem Cells Delivery

Cited by 10 publications

References 0 publications

Comparison of Wound Healing Effect of Skin Micrograft Impregnated into Two Kinds of Artificial Dermis in a Murine Wound Model

Comparison of Wound Healing Effect of Skin Micrograft Impregnated into Two Kinds of Artificial Dermis in a Murine Wound Model

Periodontal Regeneration

Isolation of adult rat kidney derived stem cells and differentiation into podocyte-like cells

Contact Info

Product

Resources

About