Promoter activation by ACGT in response to salicylic and abscisic acids is differentially regulated by the spacing between two copies of the motif

Mehrotra, Rajesh; Mehrotra, Santosh

doi:10.1016/j.jplph.2010.04.005

Cited by 40 publications

(23 citation statements)

References 28 publications

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“…ABA, JA and SA. Reference number [10] had shown that two ACGT elements separated by 25 bp were induced in response to ABA and in present study; it has been shown that PP2C like promoter with an arrangement of two ACGT elements separated by 30 bp is also induced in response to ABA.…”

Section: Resultssupporting

confidence: 65%

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AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (PP2C) like Promoter in Arabidopsis thaliana

Bhalothia¹,

Alok²,

Mehrotra³

et al. 2013

AJPS

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Genome wide analysis of Arabidopsis thaliana reveals a unique genetic arrangement of ACGT motif in the protein phosphatase 2C (PP2C) like promoter (accession number AT5G59220). In the present study, full length, 900 base pair, 500 base pair, 400 base pair and NRM deletion variants of PP2C like promoter were constructed to investigate the activity of PP2C like promoter in the presence of inducers like abscisic acid, jasmonic acid and salicylic acid. The PP2C promoter has three ACGT elements in close vicinity. They are so positioned that they are separated by a spacer of 30 base pair and 5 base pair respectively from each other. The study has shown that ACGT N30 ACGT genetic architecture is essential for the promoter to be induced in response to abscisic acid. The synergistic and antagonistic effects of cis elements were observed. AACA is a positive regulatory element in endosperm and is known to act as negative regulatory element in other tissues. In this study AACA, have been found to negatively regulate the expression of reporter gene EGFP in both induced and under uninduced conditions.

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Section: Resultssupporting

confidence: 65%

“…Reference number [10] showed that two ACGT elements separated by 25 base pair (bp) are induced by ABA. However, when separated by 5 nucleotides, they are induced by SA in transgenic tobacco.…”

Section: Introductionmentioning

confidence: 99%

AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (PP2C) like Promoter in Arabidopsis thaliana

Bhalothia¹,

Alok²,

Mehrotra³

et al. 2013

AJPS

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“…Therefore, the evolution of the MIR168 promoter sequence does not correspond closely with that of MIR168 sequences. It is reported that these G-boxes are differentially regulated by the distance between two copies of the motif (Mehrotra and Mehrotra, 2010). Moreover, the G-box copy number in a promoter, and the distance from the transcriptional start site, also result in drastically altered gene expression, causing changes in the pattern of expression and even in resistance (Mehrotra et al, 2005(Mehrotra et al, , 2013.…”

Section: Dna Sequence Variation In Mir168 Promoter Regionsmentioning

confidence: 99%

Comparative analysis of MIR168 promoters in three plant species

Yan

et al. 2016

Genet. Mol. Res.

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ABSTRACT. MicroRNAs (miRNAs) play important roles in the regulation of gene expression by post-transcriptionally targeting mRNAs for cleavage or translational repression. miR168 is a key miRNA because it regulates the expression of the slicer protein ARGONAUTE1 (AGO1), which catalyzes mRNA cleavage. Interestingly, plant miR168s are highly evolutionarily conserved; however, it is unclear whether MIR168 promoter elements and expression patterns are also conserved. Here, we isolated MIR168 promoters from monocot rice and dicot grape genomes. To determine the expression pattern, different promoters were fused to a beta-glucoronidase reporter gene and the resulting constructs were then transformed in Arabidopsis. The results revealed clear differences in the MIR168 promoter sequence of monocot and dicot plant species. Moreover, the pattern of MIR168 promoter expression differed between monocots and dicots. These results suggest that, unlike that of miR168, the MIR168 promoter is not conserved in monocots and dicots.

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“…The differential induction is expected to involve the recruitment of different bZIP transcription factors. This suggests that a change in spacing between two copies of a given motif can alter the signal pathway to which a promoter responds [2].…”

Section: Short Commentarymentioning

confidence: 99%

Cis Regulatory Elements in Regulation of Plant Gene Expression: An Overview

Mehrotra¹,

Sangwan²

2015

Gene Technol

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Promoter activation by ACGT in response to salicylic and abscisic acids is differentially regulated by the spacing between two copies of the motif

Cited by 40 publications

References 28 publications

AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (<i>PP2C</i>) like Promoter in <i>Arabidopsis thaliana</i>

AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (<i>PP2C</i>) like Promoter in <i>Arabidopsis thaliana</i>

Comparative analysis of MIR168 promoters in three plant species

Cis Regulatory Elements in Regulation of Plant Gene Expression: An Overview

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Promoter activation by ACGT in response to salicylic and abscisic acids is differentially regulated by the spacing between two copies of the motif

Cited by 40 publications

References 28 publications

AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (&lt;i&gt;PP2C&lt;/i&gt;) like Promoter in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;

AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (&lt;i&gt;PP2C&lt;/i&gt;) like Promoter in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;

Comparative analysis of MIR168 promoters in three plant species

Cis Regulatory Elements in Regulation of Plant Gene Expression: An Overview

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Product

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AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (<i>PP2C</i>) like Promoter in <i>Arabidopsis thaliana</i>

AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (<i>PP2C</i>) like Promoter in <i>Arabidopsis thaliana</i>