AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (<i>PP2C</i>) like Promoter in <i>Arabidopsis thaliana</i>

Abstract

Genome wide analysis of Arabidopsis thaliana reveals a unique genetic arrangement of ACGT motif in the protein phosphatase 2C (PP2C) like promoter (accession number AT5G59220). In the present study, full length, 900 base pair, 500 base pair, 400 base pair and NRM deletion variants of PP2C like promoter were constructed to investigate the activity of PP2C like promoter in the presence of inducers like abscisic acid, jasmonic acid and salicylic acid. The PP2C promoter has three ACGT elements in close vicinity. They are so positioned that they are separated by a spacer of 30 base pair and 5 base pair respectively from each other. The study has shown that ACGT N30 ACGT genetic architecture is essential for the promoter to be induced in response to abscisic acid. The synergistic and antagonistic effects of cis elements were observed. AACA is a positive regulatory element in endosperm and is known to act as negative regulatory element in other tissues. In this study AACA, have been found to negatively regulate the expression of reporter gene EGFP in both induced and under uninduced conditions.

Identification and Characterization of a T-DNA Promoter Trap Line of Arabidopsis thaliana Uncovers an Embryo Sac-Specific Bi-directional Promoter

“…It has been reported that Skn-1 motif in the promoter upregulates carotenogenic gene expression during flower development in Gentiana lutea (Zhu et al 2014). In A. thaliana, AACA is a positive regulatory element in the endosperm (PP2C promoter) and functions as a negative regulatory element in other tissues (Bhalothia et al 2013). The CCAAT box elements interact with transcription factor NF-Y for efficient transcription (Zanotto et al 2007).…”

Cis Regulatory Elements in Regulation of Plant Gene Expression: An Overview

“…Our work in the protein phosphatase 2C (PP2C) like promoter of Arabidopsis thaliana revealed that AACA element negatively regulated the expression of the above promoter, even in the presence of inducers [5].…”

In vitro regeneration and optimization of factors affecting Agrobacterium mediated transformation in Artemisia Pallens, an important medicinal plant

“…The optimization of genetic transformation of Artemisia species will pave way to engineer a metabolic pathway for in-vivo enhanced production of the compound of our interest. Various genetic transformation methods are available, but most frequently BBiolistic method ( Rech et al 2008;Bhalothia et al 2013) and BAgrobacteriummediated^ (Upadhyay et al 2013;Elfahmi and Chahyadi, 2014) transformation are used. Agrobacterium-mediated genetic transformation is a cost effective and easier method for transformation of plants.…”