Promising New Assays and Technologies for the Diagnosis and Management of Infectious Diseases

Mitsuma, S. F.; Mansour, Mahmoud K.; Dekker, John P.; Kim, J.; Rahman, Mohammed Ziaur; Tweed-Kent, A.; Schuetz, Philipp

doi:10.1093/cid/cis1014

Cited by 78 publications

(46 citation statements)

References 49 publications

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“…La capacidad para analizar un gran número de aislamientos simultáneamente permite incrementar el rendimiento; además, una vez cargado el instrumento, las identificaciones se pueden realizar en <1 minuto 64 . Esta mejora en el tiempo de respuesta representa un beneficio sustancial en la oportunidad del resultado y la atención del paciente 65,66 , así como en el ahorro que genera en los costos de atención 33 .…”

Section: Impacto Del Uso Del Maldi Tof Msunclassified

La espectrometría de masas MALDI-TOF en el laboratorio de microbiología clínica

Maldonado

Robledo

2017

Infect

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ResumenLos métodos fenotípicos empleados para la identificación de microorganismos dependen de procesos metabólicos que requieren de tiempos de incubación mínimos para alcanzar resultados confiables. La espectrometría de masas MALDI-TOF (desorción/ionización láser asistida por una matriz con detección de masas por tiempo de vuelo) se ha instaurado como una metodología relevante para la identificación de microorganismos mediante el análisis de proteínas, a través de la creación de un espectro de masas específico de género y especie. En esta revisión, se presenta MALDI-TOF MS como una tecnología precisa para la identificación de bacterias, levaduras, mohos, en incluso de virus, que además, permite la reducción del tiempo para obtener un resultado de identificación, que puede impactar los costos de atención y duración de la estancia hospitalaria. La identificación de microorganismos directamente de muestras biológicas y la detección de mecanismos de resistencia a antimicrobianos, prometen un mayor impacto clínico y epidemiológico con el desarrollo e implementación de esta tecnología en los laboratorios de microbiología clínica.Palabras clave: MALDI-TOF MS, espectrometría de masas, diagnóstico microbiológico, identificación de patógenos, proteómica, infección del torrente sanguíneo, infección del tracto urinario. MALDI-TOF mass spectrometry in clinical microbiology laboratories. AbstractPhenotypic methods used for the identification of microorganisms depend on metabolic processes that require minimum incubation times to achieve reliable results. For this reason, MALDI-TOF MS (Matrix Assisted Laser Desorption/Ionization Mass Spectrometry) has been established as a relevant methodology for the identification of microorganisms using analysis of proteins, through the creation of a mass spectrum specific for genus and species. In the present review, MALDI-TOF MS is presented as an accurate technology for identifying bacteria, yeasts, molds and viruses; Its use allows reduction of the time to obtain an identification result, which may impact the costs of care and length of hospital stay. The identification of microorganisms directly from biological samples and the detection of mechanisms of antimicrobial resistance, promise an additional clinical and epidemiological impact with the development and implementation of this technology in clinical microbiology laboratories.Keywords: MALDI-TOF MS, mass spectrometry, microbiological diagnostic, pathogen identification, proteomics, blood stream infection, urinary tract infection. REVISIÓN IntroducciónLa identificación de microorganismos se ha realizado tradicionalmente por métodos basados en tinciones que permiten la clasificación de la morfología microscópica con el fin de apoyar decisiones diagnósticas y terapéuticas tempranas; así como en pruebas in vitro basadas en reacciones bioquímicas utilizadas por sistemas manuales o automatizados, que integran pruebas con diferentes sustratos, incrementando la rapidez y simplicidad de la identificación. Sin embargo, estos métodos fenotí...

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Section: Impacto Del Uso Del Maldi Tof Msunclassified

La espectrometría de masas MALDI-TOF en el laboratorio de microbiología clínica

Maldonado

Robledo

2017

Infect

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“…Biomarkers have shown to be very effective in aiding diagnosis and management of hospital patients with suspected systemic bacterial infections, including community-acquired pneumonia (CAP) and sepsis [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28]. Procalcitonin (PCT) is a novel and effective marker of assumed bacterial infections that safely helps guide antibiotic therapy in acute respiratory tract infections (ARI) and sepsis in hospitals [5,[29][30][31][32][33][34][35][36][37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Economic evaluation of procalcitonin-guided antibiotic therapy in acute respiratory infections: a US health system perspective

Schuetz

Balk

Briel³

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background: Whether or not antibiotic stewardship protocols based on procalcitonin levels results in cost savings remains unclear. Herein, our objective was to assess the economic impact of adopting procalcitonin testing among patients with suspected acute respiratory tract infection (ARI) from the perspective of a typical US integrated delivery network (IDN) with a 1,000,000 member catchment area or enrollment. Methods: To conduct an economic evaluation of procalcitonin testing versus usual care we built a cost-impact

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“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] The first Red Book was published by the AAP in 1938 as an 8-page document (Fig 1). The 2012 Red Book: Report of the Committee on Infectious Diseases is the 29th edition and is an internationally recognized authoritative reference source, which has become a comprehensive, preeminent guide to diagnosis, treatment, management, and prevention of pediatric infectious diseases.…”

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confidence: 99%

“…13 The Red Book continues to evolve to keep pace with changing clinical practice and innovations in pediatric care. An application for mobile devices including smartphones and tablets was introduced, so practitioners can access the wealth of information in the Red Book at any time.…”

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confidence: 99%

The Red Book Through the Ages

2013

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The first edition of the Red Book was published in 1938. Since then, there have been numerous advances in the fields of infectious diseases and public health that have decreased morbidity and mortality of infants, children, and adolescents. Over the years, emerging pathogens and disease complexes have been described, sophisticated diagnostic techniques developed, advances in antimicrobial therapy have occurred, and immunizations have been implemented to prevent previously deadly diseases. Of the 18 diseases or organisms in the 1938 edition, 13 are now vaccine-preventable. Since inception of the Red Book, the aims of the editors have been to keep pace with these innovations and to continue to inform the medical community. These goals have made the Red Book a fundamental resource for pediatricians and other health care professionals in terms of guiding diagnosis, therapy, and prevention of infectious diseases. The list of 18 diseases or organisms originally described in the 1938 Red Book has expanded to include over 160 diseases or organisms in the 2012 edition. The pace of biomedical discovery, as well as the amount of information available and the number of methods for its delivery, will continue to accelerate in the future. Integration of information into future editions of the Red Book will ensure that practitioners continue to rely on the Red Book in its various electronic formats for clinical guidance and support. Pediatrics 2013;132:898-906

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Promising New Assays and Technologies for the Diagnosis and Management of Infectious Diseases

Cited by 78 publications

References 49 publications

La espectrometría de masas MALDI-TOF en el laboratorio de microbiología clínica

La espectrometría de masas MALDI-TOF en el laboratorio de microbiología clínica

Economic evaluation of procalcitonin-guided antibiotic therapy in acute respiratory infections: a US health system perspective

The Red Book Through the Ages

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