Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from Medicago Sativa Leaves

Krakowska-Sieprawska, Aneta; Rafińska, Katarzyna; Walczak-Skierska, Justyna; Kiełbasa, Anna; Buszewski, Bogusław

doi:10.3390/ma14112724

Cited by 15 publications

(8 citation statements)

References 56 publications

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“…The PCA analysis illustrated that the AOA of the bound extracts was mainly attributed to the phenolics, which is confirmed by their strong correlation (r = 0.822, Table S5 ). This observation is in agreement with others [ 31 , 35 ]. The distance between TPC and their AOA in soluble extract indicates those other compounds acting as antioxidants, rather than the identified phenolics [ 29 ].…”

Section: Resultssupporting

confidence: 94%

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Characterization of Forage Quality, Phenolic Profiles, and Antioxidant Activity in Alfalfa (Medicago sativa L.)

Horvat

Vuletić

Andrić

et al. 2022

Plants

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Alfalfa (Medicago sativa L.) is one of the most important forage species and is recently more in focus for human consumption mainly due to its content of bioactive phenolics. Samples of the seventeen alfalfa cultivars/populations were collected at the Agricultural Institute Osijek with the aim to evaluate their forage quality, phenolic profiles, and antioxidant potential. Significant differences (p < 0.05) existed among studied alfalfa in all analyzed traits. The cultivar OS 99 and populations L7 and L20 were characterized by high crude protein content (22.5–24.7%) and the lowest neutral (40.2–42.9%) and acid detergent fibres (33–35.5%). The soluble-free phenolics from alfalfa leaves were extracted by methanol while insoluble cell-wall bound phenolics were released by alkaline hydrolysis. The bound phenolic extract showed a stronger DPPH scavenging capacity (20.8 mg TE/g dm) than the soluble (11.4 mg TE/g dm). The HPLC data revealed that more phenolics were found in the bound (3638.0 μg/g dm) than in the soluble form (912.3 μg/g dm). In the soluble extract of the alfalfa leaves, the major compound was catechin (338.3 μg/g dm), while rutin, epicatechin, and ferulic acid were minor ones. In the bound phenolic extract, the most abundant was ferulic (2198.2 μg/g dm) and p-coumaric acid (983.7 μg/g dm), followed by myricetin, apigenin, and quercetin. The principal component analysis revealed that alfalfa cultivars/populations were better discriminated based on the data on phenolics, rather than on forage quality. The cultivars/populations Florida 66, OS 66, L 40, L 42, Seed Force 4, and Torlesse were the most interesting in terms of phenolic health-promoting characteristics.

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Section: Resultssupporting

confidence: 94%

“…), used analytical technique (HPLC, HPLC-MS, HPLC-MS/MS, etc. ), and alfalfa part (aerial or roots) [ 16 , 35 , 36 ]. Newby et al [ 37 ] determined the dominance of alkali-labile bound phenolic acids in relation to soluble ones (less than 2%).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Forage Quality, Phenolic Profiles, and Antioxidant Activity in Alfalfa (Medicago sativa L.)

Horvat

Vuletić

Andrić

et al. 2022

Plants

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“…Owing to its high extraction efficiency and high purity of extracts, SFE is widely used to extract bioactive compounds from various substances. SFE has been used to extract and analyze natural products, allowing the extraction of aroma compounds with high purity and freshness to determine the compounds conferring the flavor characteristics of plants 81,82 . In addition, SFE is referred to as a ‘green technology’ owing to its benefits in terms of environmental protection and recycling.…”

Section: Methods For Detecting Fruit Aroma Compoundsmentioning

confidence: 99%

Advances in detecting fruit aroma compounds by combining chromatography and spectrometry

et al. 2023

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Fruit aroma is produced by volatile compounds, which can significantly enhance fruit flavor. These compounds are highly complex and have remarkable pharmacological effects. The synthesis, concentration, type, and quantity of fruit aroma substances are affected by various factors, both abiotic and biotic. To fully understand the aroma substances of various fruits and their influencing factors, detection technology can be used. Many methods exist for detecting aroma compounds, and approaches combining multiple instruments are widely used. This review describes and compares each detection technology and discusses the potential use of combined technologies to provide a comprehensive understanding of fruit aroma compounds and the factors influencing their synthesis. These results can inform the development and utilization of fruit aroma substances.

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“…It has also been shown that the initial treatment of plant material with an enzyme preparation commonly used in the agricultural industry significantly increased the efficiency of the extraction of bioactive substances. The use of enzyme-assisted supercritical fluid extraction in various industries to isolate bioactive compounds from plant material is economically effective and represents an advance in modern technological processes [ 12 , 114 ].…”

Section: The Concept Of Enzymes and Enzymatic Extractionmentioning

confidence: 99%

Modern Methods of Pre-Treatment of Plant Material for the Extraction of Bioactive Compounds

et al. 2022

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In this review, recent advances in the methods of pre-treatment of plant material for the extraction of secondary metabolites with high biological activity are presented. The correct preparation of the material for extraction is as important as the selection of the extraction method. This step should prevent the degradation of bioactive compounds as well as the development of fungi and bacteria. Currently, the methods of preparation are expected to modify the particles of the plant material in such a way that will contribute to the release of bioactive compounds loosely bonded to cell wall polymers. This review presents a wide range of methods of preparing plant material, including drying, freeze-drying, convection drying, microwave vacuum drying, enzymatic processes, and fermentation. The influence of the particular methods on the structure of plant material particles, the level of preserved bioactive compounds, and the possibility of their release during the extraction were highlighted. The plant material pre-treatment techniques used were discussed with respect to the amount of compounds released during extraction as well their application in various industries interested in products with a high content of biologically active compounds, such as the pharmaceutical, cosmetics, and food industries.

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Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from Medicago Sativa Leaves

Cited by 15 publications

References 56 publications

Characterization of Forage Quality, Phenolic Profiles, and Antioxidant Activity in Alfalfa (Medicago sativa L.)

Characterization of Forage Quality, Phenolic Profiles, and Antioxidant Activity in Alfalfa (Medicago sativa L.)

Advances in detecting fruit aroma compounds by combining chromatography and spectrometry

Modern Methods of Pre-Treatment of Plant Material for the Extraction of Bioactive Compounds

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