Promising effects of exosomes isolated from menstrual blood‐derived mesenchymal stem cell on wound‐healing process in diabetic mouse model

Abstract

Wound healing is a complicated process that contains a number of overlapping and consecutive phases, disruption in each of which can cause chronic nonhealing wounds. In the current study, we investigated the effects of exosomes as paracrine factors released from menstrual blood-derived mesenchymal stem cells (MenSCs) on wound-healing process in diabetic mice. The exosomes were isolated from MenSCs conditioned media using ultracentrifugation and were characterized by scanning electron microscope and western blotting assay. A full thickness excisional wound was created on the dorsal skin of each streptozotocin-induced diabetic mouse. The mice were divided into three groups as follows: phosphate buffered saline, exosomes, and MenSC groups. We found that MenSC-derived exosomes can resolve inflammation via induced M1-M2 macrophage polarization. It was observed that exosomes enhance neoangiogenesis through vascular endothelial growth factor A upregulation.Re-epithelialization accelerated in the exosome-treated mice, most likely through NF-κB p65 subunit upregulation and activation of the NF-κB signaling pathway. The results demonstrated that exosomes possibly cause less scar formation through decreased Col1:Col3 ratio. These notable results showed that the MenSC-derived exosomes effectively ameliorated cutaneous nonhealing wounds. We suggest that exosomes can be employed in regenerative medicine for skin repair in difficult-toheal conditions such as diabetic foot ulcer. KEYWORDSangiogenesis, diabetic murine model, excisional wound, exosome, menstrual blood, mesenchymal stem cell

Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing

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Exosome loaded alginate hydrogel promotes tissue regeneration in full‐thickness skin wounds: An in vivo study

“…Results show that Alg hydrogel containing EXO considerably improved wound closure, collagen synthesis, and vessel formation at wound area compared with Alg hydrogel and sterile gauze as control groups. Previous studies have demonstrated that the EXO transplantation can elicit almost the same healing outcomes as compared to whole‐cell transplantation (Dalirfardouei, Jamialahmadi, Jafarian, & Mahdipour, ). Therefore, we hypothesized whether an appropriate delivering system in the wound area could overcome the disadvantages and risks coupled with whole‐cell therapy.…”

Endometrial and Menstrual Blood Mesenchymal Stem/Stromal Cells: Biological Properties and Clinical Application

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