Promising approaches to crystallization of macromolecules suppressing the convective mass transport to the growing crystal

Boyko, K.М.; Popov, Vladimir O.; Kovalchuk, M. V.

doi:10.1070/rcr4557

Cited by 13 publications

(10 citation statements)

References 82 publications

(76 reference statements)

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“…AcLys was crystallized using vapor diffusion technique. Microgravity conditions were produced on module Luch of International Space Station (expeditions ISS-51 and ISS-53) to decrease convection [ 18 ]and obtain high quality protein crystals. Crystals were grown at 20 °C in the following conditions: 0.2 M Li 2 SO 4 ; 0.1 M MES (2-( N -morpholino)ethanesulfonic acid), pH 6.5; 25% PEG3350.…”

Section: Materialsandmethodsmentioning

confidence: 99%

Structure of an Acinetobacter Broad-Range Prophage Endolysin Reveals a C-Terminal α-Helix with the Proposed Role in Activity against Live Bacterial Cells

Сыкилинда

Shneider

Mishkin

et al. 2018

Viruses

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Proteins that include enzymatic domain degrading the bacterial cell wall and a domain providing transport through the bacterial outer membrane are considered as prospective compounds to combat pathogenic Gram-negative bacteria. This paper presents an isolation and study of an enzyme of this class naturally encoded in the prophage region of Acinetobacter baumannii AB 5075 genome. Recombinant protein expressed in E. coli exhibits an antimicrobial activity with respect to live cultures of Gram-negative bacteria reducing the population of viable bacteria by 1.5–2 log colony forming units (CFU)/mL. However the protein becomes rapidly inactivated and enables the bacteria to restore the population. AcLys structure determined by X-ray crystallography reveals a predominantly α—helical fold similar to bacteriophage P22 lysozyme. The С-terminal part of AcLys polypeptide chains forms an α—helix enriched by Lys and Arg residues exposed outside of the protein globule. Presumably this type of structure of the C-terminal α—helix has evolved evolutionally enabling the endolysin to pass the inner membrane during the host lysis or, potentially, to penetrate the outer membrane of the Gram-negative bacteria.

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Section: Materialsandmethodsmentioning

confidence: 99%

Structure of an Acinetobacter Broad-Range Prophage Endolysin Reveals a C-Terminal α-Helix with the Proposed Role in Activity against Live Bacterial Cells

Сыкилинда

Shneider

Mishkin

et al. 2018

Viruses

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“…Hypersonic grinding by the method of quantum aerogravity technology for the production of powders has shown the applicability and usefulness of this direction in practice. To date, the best indicators were obtained by freeze-drying (sublimation) [2,6,7]. With the development and introduction of a new grinding technology, a completely new method of dynamic drying in combination with simultaneous grinding becomes available.…”

Section: Methodsmentioning

confidence: 99%

“…This is due to the fact that the designs of existing dynamic self-grinding mills are based on the kinetic energy of the rotating rotor, which depends mainly on the speed of its rotation, which cannot exceed a certain critical value. At the same time, it should be noted that the tendency to increase the productivity of grinding by installing more powerful drives on known grinding equipment necessarily leads to a decrease in their reliability and an increase in capital costs [1,2,3].…”

Section: Introductionmentioning

confidence: 99%

Convergent technologies as conceptual basis for formation of powder industry in agribusiness

Kerimov

Belinskaia

Ognev

2021

20th International Scientific Conference Engineering for Rural Development Proceedings

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The active introduction of new technologies, including those based on the application of the principles of nano-grinding, can significantly reduce energy consumption in the production of agricultural products. The scale of such developments in the world suggests the need to create a scientific concept that forms a unified systematic approach to the use of promising technologies for grinding solids and materials. To solve this problem, the authors have developed a technology for crushing agricultural raw materials, and also designed a working body for a disintegrator-type grinder. The studies carried out by the authors on the use of nanopowders in the production of feed for cattle, as well as mineral fertilizers, made it possible to formulate the goals, objectives and main directions of development of the powder industry based on the use of convergent technologies, as well as mechanisms and ways to achieve these goals. Implementation of the developed concept is of great fundamental and practical importance. It will allow to ensure, in the medium term, the transition of the economy of the agricultural sector to the large-scale application of technologies for nanomilling of raw materials, secondary raw materials and waste processing, which will significantly reduce production costs. A decrease in costs on the scale of the regional economy will be facilitated by: decrease in logistics costs for raw materials in the form of crushed microfractions; increase in productivity and a corresponding increase in profitability by improving the properties of particles, increasing their surface as a result of the use of grinding technologies; improving the quality characteristics of agricultural products.

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“…The technology for producing fine powders is based on the separation of loaded organic raw materials, followed by its grinding due to the formation of two circular counter flows [2,3,4]. The movement of material in the flow occurs at a speed of 400...700 m/s.…”

Section: Object Of Study and Methodologymentioning

confidence: 99%

Nanotechnologies in agricultural engineering: practice and prospects

et al. 2020

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The use of powdered concentrates increases the digestibility of feed components by the animal body. The biological activity of organic products increases due to the intensification of the process of useful substances extraction from them. The scientific basis for the formation of nanotechnologies is system effects, as well as features of physical, mechanical and biochemical phenomena that occur in powdered materials at the atomic and molecular levels and provide a synergistic effect. Using modern analytical tools in the field of nanoparticle research, a method for conducting search experiments has been developed, which provides for the possibility of studying the transformations occurring in the deep layers of substances and trace elements. Fine fractions participate in the formation of the structure of the final product, act as its leavening agents and sweeteners, as well as absorbers of excess moisture contained in raw materials. Hypersonic grinding using quantum aerogravity technology has shown the applicability, scientific and technical significance and prospects of this direction in the development of the powder industry.

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Promising approaches to crystallization of macromolecules suppressing the convective mass transport to the growing crystal

Cited by 13 publications

References 82 publications

Structure of an Acinetobacter Broad-Range Prophage Endolysin Reveals a C-Terminal α-Helix with the Proposed Role in Activity against Live Bacterial Cells

Structure of an Acinetobacter Broad-Range Prophage Endolysin Reveals a C-Terminal α-Helix with the Proposed Role in Activity against Live Bacterial Cells

Convergent technologies as conceptual basis for formation of powder industry in agribusiness

Nanotechnologies in agricultural engineering: practice and prospects

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