Promising application of fine bubbles (FBs) to control Aeromonas hydrophila on striped catfish seed (Pangasianodon hypophthalmus)

Subhan, Ujang; Mauladani, Syifa; Rosidah,; Suryadi, Ibnu Bangkit Bioshina; Joni, I Made

doi:10.1063/5.0003059

Cited by 3 publications

(5 citation statements)

References 11 publications

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“… Pangasianodon hypophthalmus (striped catfish) is a major freshwater species and dominates 2% of global live-weight aquaculture production ( 18 ). Nonetheless, bacterial pathogens can cause production losses ( 19 , 20 ). The bacterium that commonly causes catfish disease is Aeromonas hydrophila (AH) ( 20 – 23 ).…”

Section: Introductionmentioning

confidence: 99%

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Aeromonas hydrophila Induces Skin Disturbance through Mucosal Microbiota Dysbiosis in Striped Catfish ( Pangasianodon hypophthalmus )

Chen

Lin

Siao

et al. 2022

mSphere

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Section: Introductionmentioning

confidence: 99%

“…Nonetheless, bacterial pathogens can cause production losses ( 19 , 20 ). The bacterium that commonly causes catfish disease is Aeromonas hydrophila (AH) ( 20 – 23 ). AH is the causative agent of motile Aeromonas septicemia (MAS) in farmed striped catfish, especially in the juvenile stage ( 24 ).…”

Section: Introductionmentioning

confidence: 99%

Aeromonas hydrophila Induces Skin Disturbance through Mucosal Microbiota Dysbiosis in Striped Catfish ( Pangasianodon hypophthalmus )

Chen

Lin

Siao

et al. 2022

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“…Pangasianodon hypophthalmus , commonly named striped catfish, is a major freshwater species and dominants 2% of global live-weight aquaculture production (15). Nonetheless, bacterial pathogens can cause production loss (16, 17). The bacteria that commonly cause catfish disease is Aeromonas hydrophila (AH) (17–20).…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, bacterial pathogens can cause production loss (16, 17). The bacteria that commonly cause catfish disease is Aeromonas hydrophila (AH) (17–20). AH is the causative agent of Motile Aeromonas Septicemia (MAS) in the farmed striped catfish, especially in the juvenile stage (21).…”

Section: Introductionmentioning

confidence: 99%

Aeromonas hydrophila induces skin disturbance through mucosal microbiota dysbiosis in Pangasianodon hypophthalmus

CHEN¹,

LIN²,

SIAO³

et al. 2022

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Bacterial pathogens are well-equipped to adhere to and initiate infection in teleost fish. The fish skin mucus serves as the first barrier against environmental pathogens. The mucus harbors commensal microbes that form the microbiota, impacting the host physiological and immunological regulation. However, how the skin mucosal microbiota responds to the presence of pathogens remains largely unexplored. Thus, little is known about the status of skin mucus prior to the infection with noticeable symptoms. In this study, we aim to investigate the interaction between pathogen and skin mucosal microbiota, as well as the fish skin immune responses in the presence of pathogens. By challenging striped catfish (Pangasianodon hypophthalmus) with different concentrations of bacterial pathogen Aeromonas hydrophila (AH), the skin immune response and the mucosal microbiota were examined by qPCR and 16S rRNA gene sequencing analysis. We found a pathogen concentration able to stimulate the skin immune response associated with the significant mucosal microbiota change and re-confirmed with the ex vivo fish skin model. Further analysis indicated that the change was attributed to the significant increase in opportunistic pathogens over AH. We concluded that the presence and increase of AH results in dysbiosis of mucosal microbiota that can stimulate skin immune response. We believe our work can shed some light on host-pathogen-commensal microbiota interaction and therefore contribute to aquaculture infection prevention.

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“…Refer to Hendry et al (2011), the high variation of a specific character (disease resistance) in the population suggested that a selection method may be conducted to improve the character. In the natural disease outbreak, there were 10-20% of fish survive (survivor) (Subhan et al 2020). Furthermore, the survivor can establish a based population to develop the SPR population (Moss et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Assessing the susceptibility of the selected gourami (Osphronemus goramy) to Aeromonas hydrophila

2021

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Abstract. Febrianti R, Khasani I, Rosada KK. 2021. Assessing the susceptibility of the selected gourami (Osphronemus goramy) to Aeromonas hydrophila. Nusantara Bioscience 13: 111-120. A breeding program to improve the growth performance of the gourami fish was carried out through selection methods that produced faster growth gourami (selected population). The purpose of this study was to determine the susceptibility of the selected gourami to Aeromonas hydrophila infection based on tolerance limits (LD50) and investigated clinical signs post-injection of the pathogenic bacteria. The challenge test by intramuscular injection of A. hydrophila was done to the fingerling fish (15-20 g) for 14 days post-infection. The population of the tested fish was obtained from six families, selected gourami (SP), and non-selected control (CP) groups. Phosphate buffered saline (PBS) as control and several doses of the pathogen, 102, 104, 105, 106, 107, and 108 CFU/mL of A. hydrophila, were injected into the fish. Fish mortality and clinical signs were observed daily. The fish mortality was confirmed by isolating bacteria in the fish which showed clinical signs, followed by biochemical characterization of the isolated bacteria using API 20E and PCR. The LD50 of A. hydrophila to the selected population (9.70 × 105 CFU/mL) was higher than that of the control (6.50 × 104 CFU/mL). On the final day of the test, the accumulation mortality of CP (63.33±5.77%) higher than that in SP (33.33±5.77%). Based on the output test statistics, it was known that there was a significant difference between the mortality and clinical signs of SP and CP. The data suggested that the selected gourami were more resistant to A. hydrophila infection than that of the control. The A. hydrophila infection caused most of the major clinical signs, including mass mortality of the fish. The biochemical and PCR test ensure that fish mortality was caused by A. hydrophila infection.

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Promising application of fine bubbles (FBs) to control Aeromonas hydrophila on striped catfish seed (Pangasianodon hypophthalmus)

Cited by 3 publications

References 11 publications

Aeromonas hydrophila Induces Skin Disturbance through Mucosal Microbiota Dysbiosis in Striped Catfish ( Pangasianodon hypophthalmus )

Aeromonas hydrophila Induces Skin Disturbance through Mucosal Microbiota Dysbiosis in Striped Catfish ( Pangasianodon hypophthalmus )

Aeromonas hydrophila induces skin disturbance through mucosal microbiota dysbiosis in Pangasianodon hypophthalmus

Assessing the susceptibility of the selected gourami (Osphronemus goramy) to Aeromonas hydrophila

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