Prolonged survival by combined treatment with granulocyte colony‐stimulating factor and dipeptidyl peptidase <scp>IV</scp> inhibitor in a rat small‐for‐size liver transplantation model

Hsu, Li-Wen; Nakano, Toshiaki; Huang, Kuang-Tzu; Chen, Chien-Chih; Chen, Kuang‐Den; Lai, Chia-Yun; Yang, Sheng‐Hsiung; Lin, Chih‐Che; Wang, Chih‐Chi; Cheng, Yu‐Fan; Chiu, King-Wah; Kuo, Yur‐Ren; Goto, Shigeru

doi:10.1111/hepr.12413

Cited by 3 publications

(8 citation statements)

References 64 publications

(120 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, therefore, we first determined to re‐evaluate the rate of recipient‐derived hepatocytes after allogenic liver transplantation using cell culture of hepatocytes isolated from transplanted livers. In contrast with implications from previous studies and our expectation, the results show practically no evidence of recipient‐derived hepatocytes after allogenic and small graft liver transplantation. A possible explanation for the results reported herein is that the hepatocyte isolation and cell culture procedures selectively eliminated recipient‐derived cells for unknown reasons.…”

Section: Discussioncontrasting

confidence: 99%

“…In contrast, studies of liver transplantation in rats demonstrated that the allogenic rejection reaction promoted hepatocyte replacement by extrahepatic cells . However, the experimental conditions and the methods used to detect the extrahepatic cell‐derived hepatocytes varied across these studies.…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, several studies have suggested that allogenic liver transplantation promotes liver regeneration by recipient‐derived cells or by the stem cells administrated in rat models . After allogenic transplantation, intraportal administration of bone marrow‐derived stem cells from syngenic rats (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…the same strain as the recipient rat) ameliorated liver damage and substantially replaced the graft hepatocytes . Experiments from other groups have demonstrated substantial replacement of endogenous hepatocytes by donor‐derived cells after small liver graft transplantation with or without mobilization of bone marrow cells . Because immunosuppressants suppress the emergence of recipient‐derived hepatocytes, the rejection reaction was considered a necessary component for enhanced recruitment of donor‐derived stem and/or progenitor cells …”

Section: Introductionmentioning

confidence: 99%

“…In previous studies, green fluorescent protein (GFP) has been used as a molecular tag to determine the origin of the cells being examined. In addition, robust replacement of hepatocytes has been demonstrated on the basis of the fluorescence intensity of the whole liver, as well as the use of rat major histocompatibility complex (MHC) class I antibody (RT1A), polymerase chain reaction and FISH for the Y chromosome . In the present study, we also used GFP as a molecular tag, after allogenic liver transplantation between GFP transgenic (Tg) and wild‐type rats.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Re‐evaluation of hepatocyte replacement by recipient‐derived cells after allogenic liver transplantation: Discrepancy between clinical observations and a rat model

Maeda

Namikawa

Tsuda

et al. 2016

Hepatology Research

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Re‐evaluation of hepatocyte replacement by recipient‐derived cells after allogenic liver transplantation: Discrepancy between clinical observations and a rat model

Maeda

Namikawa

Tsuda

et al. 2016

Hepatology Research

View full text Add to dashboard Cite

show abstract

Combination of a CD26 Inhibitor, G-CSF, and Short-term Immunosuppressants Modulates Allotransplant Survival and Immunoregulation in a Rodent Hindlimb Allotransplant Model

Chen

Wang

et al. 2021

Transplantation

Self Cite

View full text Add to dashboard Cite

Original Basic Science-GeneralBackground. Recent studies have demonstrated that inhibition of CD26 potentiates stromal cell-derived factor-1α (SDF-1α), promotes tissue regeneration, and suppresses the rejection of organ transplants. This study investigated whether the combination of a CD26 inhibitor (CD26i) with granulocyte colony-stimulating factor (G-CSF) and short-term immunosuppressants modulates vascularized composite tissue allotransplant survival in a rodent orthotopic hindlimb allotransplant model. Methods. The hindlimb allotransplantation from Brown-Norway to Lewis rats was divided into 4 groups. Group 1 (controls) did not receive any treatment. Group 2 was treated with short-term antilymphocyte serum (ALS) and cyclosporine-A (CsA). Group 3 was administrated CD26i and G-CSF. Group 4 received a combination of CD26i/G-CSF/ALS/CsA. Each subgroup comprised 10 rats. Peripheral blood and sampling of transplanted tissues were collected for immunological and histological analysis. Results. The results revealed that allotransplant survival was found to be significantly prolonged in group 4 with CD26i/G-CSF/ALS/CsA treatment compared with those in the other groups. The interleukin-10 and transforming growth factor-βl levels, the percentage of CD4 + /CD25 + /FoxP3 + T cells, as well as the levels of SDF-1α expressions were significantly increased in group 4 compared with those in the other groups. Group 4 revealed a statistical increase in the percentage of donor cells (RT1 n ) expression in the recipient peripheral blood, and the mixed lymphocyte reaction showed hyporesponsiveness of the T cells to donor alloantigens. Conclusion. The combination of CD26i/G-CSF and short-term immunosuppressants prolongs allotransplant survival by inducing immunoregulatory effects and enhancing the percentage of SDF-1α expression. This immunomodulatory approach has great potential as a strategy to increase vascularized composite allotransplantation survival.

show abstract

Translational medical research and liver transplantation: systematic review

Nacif

Kim

Galvão

et al. 2018

Transl. Gastroenterol. Hepatol

View full text Add to dashboard Cite

Translational medicine has become a priority, but there is still a big difference between the arrival of new treatments and investment. Basic science should not be neglected because the translation from basic research is not sustained in the absence of basic research. The purpose of this literature review was to analyze the translational medicine in the liver transplant field: liver ischemia-reperfusion injury (IRI), immunosuppression, clinical and surgical complications, small-for-size syndrome (SFSS), rejection, and ongoing innovations (liver machine, liver preservation, artificial livers, and regenerative medicine).We performed a systematic literature review that were updated in October 2016. The searches were performed in the Cochrane Central Register of Controlled Trials and Review, PubMed/Medline, Embase, and LILACS databases. All the selected studies on the management of translational medical research in liver transplantation (LT) were analyzed. Initially the search found 773 articles. Methodological viewing and analysis of the articles, followed by the application of scientific models, including translational medicine in the liver transplant field. In conclusions, this review demonstrates the application of scientific research with translation medical benefits regarding the LT. The literature has a great tendency, improvements and investments in the study of translational medicine in LT. Innovative studies and technologies from basic science help to clarify clinical doubts. Moreover, evidence increases the importance of scientific research in quality of clinical practice care.

show abstract

Prolonged survival by combined treatment with granulocyte colony‐stimulating factor and dipeptidyl peptidase IV inhibitor in a rat small‐for‐size liver transplantation model

Abstract: Our results suggest that combined treatment with G-CSF and DPP-IV inhibitor may synergistically induce migration and differentiation of recipient-derived stem cells into the hepatic progenitor cells, resulting in the amelioration of SFS liver graft syndrome.

Cited by 3 publications

References 64 publications

Re‐evaluation of hepatocyte replacement by recipient‐derived cells after allogenic liver transplantation: Discrepancy between clinical observations and a rat model

Re‐evaluation of hepatocyte replacement by recipient‐derived cells after allogenic liver transplantation: Discrepancy between clinical observations and a rat model

Combination of a CD26 Inhibitor, G-CSF, and Short-term Immunosuppressants Modulates Allotransplant Survival and Immunoregulation in a Rodent Hindlimb Allotransplant Model

Translational medical research and liver transplantation: systematic review

Contact Info

Product

Resources

About