Prolongation of Segmental Pancreatic Allografts in Dogs Receiving Cyclosporin A

Smit

et al. 1988

This study assessed the early postoperative pancreatic endocrine function after intraperitoneal segmental hetero- and ortho-topic pancreatic allotransplantation in hemipancreatectomized, nonimmunesuppressed chacma baboons. Hemipancreatectomized animals remained normoglycaemic but rendered significantly reduced K values and insulin release during IVGTT, findings consistent with major pancreatic resection. Segmental hetero- or ortho-topic pancreatic transplantation did not improve reduced K values and hypoinsulinaemia following hemipancreatectomy although orthotopically sited grafts rendered the best glucose tolerance test curves. Glucagon output during IVGTT remained the same in both transplant models. It is concluded that the postoperative hormonal response was similar in both orthotopic and heterotopic transplant recipients, which indicates that drainage of graft venous effluent into the portal circulation has no advantage over systemic insulin drainage as reflected in this "diabetic" model.

Section: Resultssupporting

confidence: 87%

Endocrine function after hetero‐ and ortho‐topic segmental pancreatic transplantation in primates

Smit

et al. 1988

“…This may partially be explained by the fact that the primate is predominantly vegetarian in its dietary habits. These findings are in contrast to those seen in pancreatectomized dogs in which dietary supplementation is mandatory after transplantation of the pancreas [2]. In our autograft studies the animals gained weight.…”

Section: Discussioncontrasting

confidence: 76%

“…In autograft recipients followed up for 6 months, diarrhoea was not observed despite the fact that exocrine enzymes were not supplemented [3]. This may partially be explained by the fact that the primate is predominantly vegetarian in its dietary habits.…”

Section: Discussionmentioning

confidence: 97%

Diabetes in pancreatectomized baboons: A model for pancreatin transplantation studies

Louw

et al. 1987

This study was designed to assess plasma glucose levels (PLG) and insulin release in totally pancreatectomized baboons when challenged with intravenous glucose administration (IVGTT). Ten animals (Papio ursinus) were used and duodenectomy was intentionally avoided. The PLG at death was 18.0 +/- mmol/L, and the mean K-value within 3 days after pancreatectomy was 0.4% +/- 0.2%, indicating a significant impairment of glucose disappearance from the blood when compared to the control animals (P less than 0.01). Plasma insulin levels before and after stimulation with glucose were below the lowest level of insulin assay sensitivity. We conclude that in the primate, as in the dog, surgical pancreatectomy produced a reliable diabetic model, which is uniformly lethal if left untreated.

“…Subsequently other workers, including Du Toit et al showed similar results in totally pancreatectomized dogs [7]. However, preliminary results in primates have demonstrated that CSA is not as potent an immunosuppressive agent as was previously reported in dogs [8].…”

Section: Introductionmentioning

confidence: 62%

Significant prolongation of segmental pancreatic allograft survival in two species

1988

A study was conducted to assess the suppression of segmental pancreatic allograft rejection by cyclosporine (CSA) alone in baboons and dogs, and subtotal marrow irradiation (TL1) alone and TL 1 in combination with CSA in baboons. Total pancreatectomy in the dog and primate provided a reliable diabetic model, induced an absolute deficiency of insulin and was uniformly lethal if not treated. Continuous administration of CSA in baboons resulted in modest allograft survival. As in baboons, dogs receiving CSA 25 mg/kg/d rendered moderate graft prolongation but a dose of 40 mg/kg/d resulted in significant graft survival (greater than 100 days) in 5 of 8 allograft recipients. Irradiation alone resulted in minimal baboon pancreatic allograft survival of 20 baboons receiving TL1 1,000 rad and CSA, 3 had graft survival greater than of 100 days. Of 15 baboons receiving TL1 800 rad and CSA, 6 had graft survival of greater than 100 days. In conclusion, CSA administration in dogs and TL1 in combination with CSA in baboons resulted in highly significant segmental pancreatic allograft survival.