Prolongation of bactericidal efficiency of chitosan — Bioactive glass coating by drug controlled release

Zarghami, Vahid; Ghorbani, Mohammad; Bagheri, Kamran Pooshang; Shokrgozar, Mohammad Ali

doi:10.1016/j.porgcoat.2019.105440

Cited by 18 publications

(18 citation statements)

References 58 publications

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“…Mass spectrometry was performed by the company to confirm the molecular weight of synthetic Melittin. The peptide concentration and its purity was respectively reconfirmed using bicinchoninic acid assay (BCA) and RP-HPLC as described earlier [ 46 ]. Four µl of Melittin was drop-casted over coatings and dried at ambient conditions for 3 h.…”

Section: Methodsmentioning

confidence: 99%

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Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline

Zarghami

Ghorbani

Bagheri

et al. 2022

J Mater Sci: Mater Med

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Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants. The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection. Chitosan/bioactive glass nanoparticles/tetracycline composite coatings were deposited on hydrothermally etched titanium substrate. Melittin was then coated on composite coatings by drop casting method. The surfaces were analyzed by FTIR, XRD, and SEM instruments. Tetracycline in coatings revealed multifunctional behaviors include bone regeneration and antibacterial activity. Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings. Melittin increased the proliferation of MC3T3 cells. Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria. In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity.

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Section: Methodsmentioning

confidence: 99%

“…Antimicrobial activity of the specimens was tested against Methicillin-resistant Staphylococcus aureus (MRSA) bacteria strain according to our previous study [ 46 ]. Briefly, MRSA bacteria were cultured overnight.…”

Section: Methodsmentioning

confidence: 99%

Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline

Zarghami

Ghorbani

Bagheri

et al. 2022

J Mater Sci: Mater Med

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“…Vancomycin (Van) is a glycopeptide antibiotic. It has a good antibacterial activity for most Gram-positive bacteria due to the inhibition of the growth and reproduction of bacteria [ 65 , 66 ]. Therefore, Van is widely used to promote implant antibacterial activity and osseointegration capability [ 67 ].…”

Section: Local Drug Delivery Systems With Ti-based Implantsmentioning

confidence: 99%

Construction of Local Drug Delivery System on Titanium-Based Implants to Improve Osseointegration

et al. 2022

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Titanium and its alloys are the most widely applied orthopedic and dental implant materials due to their high biocompatibility, superior corrosion resistance, and outstanding mechanical properties. However, the lack of superior osseointegration remains the main obstacle to successful implantation. Previous traditional surface modification methods of titanium-based implants cannot fully meet the clinical needs of osseointegration. The construction of local drug delivery systems (e.g., antimicrobial drug delivery systems, anti-bone resorption drug delivery systems, etc.) on titanium-based implants has been proved to be an effective strategy to improve osseointegration. Meanwhile, these drug delivery systems can also be combined with traditional surface modification methods, such as anodic oxidation, acid etching, surface coating technology, etc., to achieve desirable and enhanced osseointegration. In this paper, we review the research progress of different local drug delivery systems using titanium-based implants and provide a theoretical basis for further research on drug delivery systems to promote bone–implant integration in the future.

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“…Accordingly, it has been found that the combination of antimicrobial peptides (AMPs) with conventional antibiotics can be a promising strategy against MDR bacterial pathogens (Zusman et al, 2013;Schmid et al, 2019). Currently, AMPs are found as novel developing antibacterial agents that could serve as an alternative to antibiotics (Bardbari et al, 2018;Memariani et al, 2018;Aghazadeh et al, 2019;Akbari et al, 2019;Bevalian et al, 2021;Zarghami et al, 2021a). Furthermore, the application of AMPs along with conventional antibiotics usually enhances the results of mono drug therapies (Bardbari et al, 2018;Akbari et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Highly Synergistic Effects of Melittin With Vancomycin and Rifampin Against Vancomycin and Rifampin Resistant Staphylococcus epidermidis

et al. 2022

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Methicillin-resistant Staphylococcus epidermidis (MRSE) strains are increasingly emerging as serious pathogens because they can be resistant to many antibiotics called multidrug resistance (MDR) that limit the therapeutic options. In the case of vancomycin- and rifampin-resistant MDR-MRSE, the physicians are not allowed to increase the doses of antibiotics because of severe toxicity. Accordingly, we investigated the synergistic activity of melittin antimicrobial peptide with vancomycin and rifampin against vancomycin-resistant, and rifampin-resistant MDR-MRSE isolates. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), fractional inhibitory concentration index (FICi), and fractional bactericidal concentration index (FBCi) of antimicrobial agents against isolates were determined. Coagulate activities and serum and salt stability as well as melittin cytotoxicity on the human embryonic kidney (HEK) 293 cells and human red blood cells (RBCs) at their synergistic concentrations. MIC and MBC values for melittin were in the range of 0.312–2.5 and 0.312–5, respectively. Results also showed that the interaction of melittin with drugs was highly synergistic in which the geometric means of FICi and FBCi were < 0.5. Induced synergism led to a decrease in melittin, rifampin, and vancomycin concentrations by 8–1,020, 2–16, and 4–16-folds, respectively. This phenomenon caused a reduction in melittin toxicity by which the synergistic concentration of melittin needed to kill bacteria did not show cytotoxicity and hemolytic activity. Besides, no coagulation activity was found for the synergistic and alone concentrations of melittin in both Prothrombin Time (PT) and Partial Thromboplastin Time (PTT). Interestingly, the antibacterial activity of melittin in Mueller Hinton Broth (MHB) containing human serum did no significant differences between MIC and MBC values of melittin in MHB and MHB containing 10% human serum. The present findings showed that the therapeutic index of melittin was improved by 32.08- and 12.82-folds when combined with vancomycin and rifampin, respectively. Taken together, the obtained data show that melittin alone was effective against MDR-MRSE isolates and this antimicrobial peptide showed highly synergistic effects with vancomycin and rifampin without causing toxicity. Therefore, the combination of melittin and traditional antibiotics could be a promising strategy for the treatment of infections caused by MDR-MRSE.

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Prolongation of bactericidal efficiency of chitosan — Bioactive glass coating by drug controlled release

Cited by 18 publications

References 58 publications

Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline

Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline

Construction of Local Drug Delivery System on Titanium-Based Implants to Improve Osseointegration

Highly Synergistic Effects of Melittin With Vancomycin and Rifampin Against Vancomycin and Rifampin Resistant Staphylococcus epidermidis

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