Proline metabolism in cherry tomato exocarp in relation to temperature and solar radiation

Rosales, Miguel A.; Ríos, Juan J.; Castellano, Rosa; López-Carrión, A. I.; Romero, Luís; Ruiz, Juan M.

doi:10.1080/14620316.2007.11512299

Cited by 19 publications

(15 citation statements)

References 38 publications

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“…The insoluble and soluble fractions were washed twice with 960 and 700 mL L −1 ethanol respectively. Both fractions were centrifuged at 3500 × g for 10 min and the supernatants were stored at 4 °C for subsequent determination of free proline30 according to the method proposed by Paquin and Lechasseur 31…”

Section: Methodsmentioning

confidence: 81%

“…Table 4 shows higher proline contents in cherry tomato fruits at the third sampling in both greenhouses, again suggesting stress in the plant tissue,16, 40 and more specifically under heat and light stress 41, 42. These results agree with those of a previous study in which high temperature and intensified solar radiation provoked a large accumulation of proline in the exocarp of cherry tomato fruits 30. Furthermore, the proline content was greater in the parral greenhouse over the entire production cycle (Table 4), coinciding with higher temperature, solar radiation and VPD values (Fig 1).…”

Section: Resultsmentioning

confidence: 99%

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Environmental conditions in relation to stress in cherry tomato fruits in two experimental Mediterranean greenhouses

et al. 2009

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BACKGROUND: Considering the economic importance of tomato and its nutritional benefits to human health, a study was conducted on how different environmental factors (temperature, solar radiation and vapour pressure deficit (VPD)) influence hydrogen peroxide detoxification and several stress indicators in cherry tomato (Solanum lycopersicum cv. Naomi) fruits grown in two experimental Mediterranean greenhouses of parral (low-technology) type and multispan (high-technology) type.

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Section: Methodsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Environmental conditions in relation to stress in cherry tomato fruits in two experimental Mediterranean greenhouses

et al. 2009

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“…This appears to be supported by Jiménez-Bremont et al (2006) who reported that exogenous PAs decreased Pro accumulation in salinity-exposed Phaseolus vulgaris L. The Pro degradation seems to be beneficial in the response to stress, given that the degradation of Pro to glutamate generates reducing equivalents that support mitochondrial oxidative phosphorylation (Hare and Cress 1997). Furthermore, the proline dehydrogenase activity is capable of consuming O2 •- (Hare and Cress 1997), and perhaps could reduce the oxidizing power of the cell and in turn possibly generate ROS (Rosales et al 2007, López-Carrión et al 2008. On the other hand, SNP appears to be capable of mitigating damage associated with salinity stress by reducing oxidative stress and inducing Pro degradation, mechanisms that permit the plant to adapt under these conditions (López-Carrión et al 2008).…”

Section: Resultsmentioning

confidence: 99%

Effects of foliar applications of nitric oxide and spermidine on chlorophyll fluorescence, photosynthesis and antioxidant enzyme activities of citrus seedlings under salinity stress

2018

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The effects of exogenous sodium nitroprusside (SNP), as nitric oxide donor, and spermidine (Spd) on growth and photosynthetic characteristics of Bakraii seedlings (Citrus reticulata × Citrus limetta) were studied under NaCl stress. In citrus plants, SNP-and Spd-induced growth improvement was found to be associated with reduced electrolyte leakage, malondialdehyde, hydrogen peroxide content, and leaf Na + and Clconcentration. However, we found increased leaf Ca 2+ , Mg 2+ , and K + concentrations, relative water content, chlorophyll fluorescence parameters, antioxidant enzyme activities, such as ascorbate peroxidase, catalase, superoxide dismutase and peroxidase, as well as higher photosynthetic rate, intercellular CO 2 concentration, stomatal conductance, and transpiration rate under saline regime. Foliar application of SNP and Spd alone mitigated the adverse effect of salinity, while the combined application proved to be even more effective.

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“…For the extraction and assay of PDH, fresh leaves or roots (0.5 g) were homogenized in 50 mM Tris-HCl buffer containing 7 mM MgCl 2 , 0.6 M KCl, 3 mM EDTA, 1 mM dithiothreitol, and 5% (w/v) polyvinylpolypyrrolidone, crosslinked, and adjusted to a pH of 7.4. The homogenate was filtered and centrifuged at 39,000 g n for 20 min at 4°C (Rosales et al, 2007). The supernatant was used to determine PDH activity.…”

Section: Methodsmentioning

confidence: 99%

Effect of Nitric Oxide on Proline Metabolism in Cucumber Seedlings under Salinity Stress

Fan¹,

Du²,

Guo³

2012

J. Amer. Soc. Hort. Sci.

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Nitric oxide (NO), an endogenous signaling molecule in plants and animals, mediates responses to abiotic and biotic stresses. This study was conducted in a nutrient solution to investigate 1) the effects of exogenous sodium nitroprusside (SNP), an NO donor, on free proline (Pro) and protein content; and 2) the enzymes involved in Pro metabolism [pyrroline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (PDH)] in cucumber (Cucumis sativus) seedling leaves and roots under NaCl stress. The results showed that the increases in free Pro and protein were significantly higher in the 50 mm NaCl solution but highly significant with the addition of 100 μM SNP to the 50 mm NaCl solution for the entire treatment period. Moreover, leaves maintained higher levels of free Pro and protein content than roots throughout the experiments. The P5CS activity increased in the saline treatment compared with the control, and this increase was greater in the 50 mm NaCl + 100 μM SNP solution than in the other treatments. On the other hand, the PDH activity was inhibited under NaCl stress but the reduction in activity was greater in the 50 mm NaCl + 100 μM SNP solution than in the others. These findings suggest that Pro metabolism was significantly altered during the exogenously applied NO under salt stress and that this alteration prompted the accumulation of higher levels of free Pro, which, in turn, maintained the turgor in the cucumber seedlings and helped protect them from salt stress. Moreover, the toxic effects generated by 50 mm NaCl were partially overcome by the application of NO, which could be used as a potential growth regulator to improve plant salinity tolerance. Therefore, it was concluded that NO could alleviate salinity damage in cucumber seedlings by regulating Pro metabolism. Overall, the adverse effects of salt stress could be lessened by the exogenous application of NO to cucumber seedlings.

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Proline metabolism in cherry tomato exocarp in relation to temperature and solar radiation

Cited by 19 publications

References 38 publications

Environmental conditions in relation to stress in cherry tomato fruits in two experimental Mediterranean greenhouses

Environmental conditions in relation to stress in cherry tomato fruits in two experimental Mediterranean greenhouses

Effects of foliar applications of nitric oxide and spermidine on chlorophyll fluorescence, photosynthesis and antioxidant enzyme activities of citrus seedlings under salinity stress

Effect of Nitric Oxide on Proline Metabolism in Cucumber Seedlings under Salinity Stress

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