Proline Accumulation as a Symptom of Drought Stress in Maize: A Tissue Differentiation Requirement

Ibarra-Caballero, Jorge; Villanueva-Verduzco, Clemente; Molina-Galán, José D.; Sánchez‐de‐Jiménez, E.

doi:10.1093/jxb/39.7.889

Cited by 75 publications

(28 citation statements)

References 21 publications

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“…2B). A comparable result has previously been found in maize seedlings experiencing drought (Ibarra‐Caballero et al 1988). These authors concluded that the proline response was a symptom of drought.…”

Section: Resultssupporting

confidence: 90%

Proline accumulation in canola leaf discs subjected to osmotic stress is related to the loss of chlorophylls and to the decrease of mitochondrial activity

Gibon

Sulpice

Larher

2000

Physiologia Plantarum

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Many stress studies use simplified experimental models like leaf discs, but the validity of such approaches remains a matter of debate, especially when attempts are made to obtain suitable biotests. We report here the analysis of the resistance of canola leaf discs to osmotic stress, as related to the accumulation of proline. For that purpose, the explanted tissues were incubated for 24 h under continuous light and at 28°C on polyethylene glycol solutions of external potentials (Πext) ranging from −0.1 to −8.0 MPa. Proline content, water deficit and chlorophyll content were quantified. The cell membrane stability, which is a structural component of viability, was assessed according to electrolyte leakage. Biomembrane oxidative damage was measured through the production of malondialdehyde and the mitochondrial activity was quantified by assessing the ability of the tissues to reduce 2,3,5‐triphenyltetrazolium chloride. Although the water deficit of the tissues reached high values (i.e. up to 70%), the cell membrane stability remained high. Furthermore, the oxidative damage to biomembranes was quite low, even in highly dehydrated tissues. In contrast, osmotic stress induced a significant decrease in the chlorophyll content and a strong accumulation of proline. These two processes each reached a maximum at a Πext of −3.0 MPa, although the tissues appeared to be viable at even lower Πext, suggesting they are not responsible for primary resistance. The mitochondrial activity was strongly decreased in the stressed leaf discs, although this activity was at least partially restored in tissues incubated for 24 h on a non‐stressing medium. It appears that the cell components of the osmotic stress resistance assessed observed in this study do not reflect the modes of resistance of an intact plant.

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Section: Resultssupporting

confidence: 90%

Proline accumulation in canola leaf discs subjected to osmotic stress is related to the loss of chlorophylls and to the decrease of mitochondrial activity

Gibon

Sulpice

Larher

2000

Physiologia Plantarum

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“…In addition, Fazeli et al (2006) concluded that plants actively accumulate proline that can enhance the plant’s water‐holding capacity and reduce drought stress by regulating the osmotic potential of the plant. In contrast, Ibarra‐Caballero et al (1988) showed that proline accumulation was not directly related to drought resistance in maize ( Zea mays L.). Increases in proline content under drought could be due to induction of proline biosynthesis as well as inhibition of its oxidation under water stress (Fazeli et al, 2006).…”

Section: Resultsmentioning

confidence: 93%

Selecting Sesame Genotypes for Drought Tolerance Based on Some Physiochemical Traits

et al. 2014

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To assess drought tolerance, an understanding of sesame (Sesamum indicum L.) physiological properties is needed. e objective of this study was to determine the physiological responses of sesame accessions to drought stress. e experiment was conducted as a two-way factorial arranged in a randomized complete block design with three replications. All of the measured traits were accession-and drought-level-speci c and discriminated the accessions into tolerant (Isfahan4, Rashtkhar, and Borazjan), moderately tolerant (Isfahan1, Ahvaz, Shiraz, Ardestan, and Kal), and sensitive (Markezi, Ultan, Khaf2, Khaf1, and Shahreza) groups. e results also showed that Khaf2 and Isfahan4 were the best accessions under normal and drought conditions, respectively. In addition, based on the correlation coe cient of the traits with seed yield, root proline and leaf carotenoid content were the superior markers with regard to drought tolerance in sesame. is study showed that accessions' di erences in drought tolerance could be attributed to the ability of sesame plants to accumulate metabolites under drought conditions, and these metabolites could be a useful tool for depicting water-de cit tolerance of sesame genotypes, which could be useful to plant breeders for selecting and developing drought-tolerant cultivars. ).Abbreviations: ROS, reactive oxygen species; RWC, relative water content; SSI, stress susceptibility index. Consequently, the objectives of this study were to determine the eff ects of drought stress on leaf relative water, chlorophyll, carotenoid, soluble sugar, and leaf and root proline contents and seed yield of sesame genotypes and to rank them for their drought tolerance based on these traits. MATERIALS AND METHODS Plant Material and TreatmentsTh e experiment was a two-way factorial (3 ´ 13) arranged in a randomized complete block design with three replicates; each replicate contained four plants (12 plants per treatment were used for various parameter measurements that were sampled). Th e fi rst factor was three drought levels (55% [I 1 ], 75% [I 2 ], and 85% [I 3 ] depletion of the available soil water) and the second factor was 13 sesame genotypes (Ultan, Khaf2,

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“…These results indicate that at HA 3 and CaN 3 concentrations, sesame seedlings procured a water stress. In fact, osmotic adjustment through the accumulation of cellular solutes, such as proline and soluble sugars, has been suggested as one of the possible mechanisms for overcoming osmotic stress caused by water deficit (Caballero et al, 2005). Therefore, sesame seedlings must synthesize soluble sugars and proline to survive and to make its osmoregulation.…”

Section: The Results Reported Inmentioning

confidence: 99%

Response of Sesame Seedlings to Different Concentrations of Humic Acids or Calcium Nitrate at Germination and Early Growth

Souguir¹,

Chérif²

2017

Cercetari Agronomice in Moldova

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ABSTRACT. This study was conducted in order to evaluate the response of sesame seeds (Sesamum indicum L.) to organic or mineral fertilization. The effects of two fertilizers, which were calcium nitrate and humic acids were studied separately at germination and early seedlings growth. Different concentrations of humic acids (HA 0 : 0, HA 1 : 500, HA 2 : 1000 and HA 3 : 2000 mg L -1 ) or calcium nitrate (CaN 0 : 0, CaN 1 : 50, CaN 2 : 100 and CaN 3 : 200 mg L -1 ) were applied distinctly to the growth media. The experimental design was accomplished in a completely randomized block design with three replications. Germination measurements, seedlings length, fresh seedling weight, total chlorophylls and carotenoids, total soluble proteins and sugars were determined. Results showed that humic acids applied at 1000 mg L -1 or calcium nitrate applied at 100 mg L -1 reduced the mean germination time (3.5 and 3.61 days) and had the highest germination index (GI) and the highest coefficient of velocity (CV). The longest seedling was obtained at the concentrations HA 2 and CaN 2 (+22%). Total chlorophyll and carotenoids were significantly higher in seedlings receiving the HA 2 and CaN 2 and these treatments increased total soluble proteins content by 32%. Higher concentrations of humic acids or calcium nitrate HA 3 and CaN 3 delayed germination and enhanced proline and total soluble sugars, respectively, by 42% and 46%, compared to control. These preliminary results indicated that the use of fertilizers should be optimized and they can be transformed at high level to an abiotic stress menacing plant productivity. On the other hand, suitable concentrations of fertilizers can be used in the future as a remedy to improve growth under abiotic stress.

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Proline Accumulation as a Symptom of Drought Stress in Maize: A Tissue Differentiation Requirement

Cited by 75 publications

References 21 publications

Proline accumulation in canola leaf discs subjected to osmotic stress is related to the loss of chlorophylls and to the decrease of mitochondrial activity

Proline accumulation in canola leaf discs subjected to osmotic stress is related to the loss of chlorophylls and to the decrease of mitochondrial activity

Selecting Sesame Genotypes for Drought Tolerance Based on Some Physiochemical Traits

Response of Sesame Seedlings to Different Concentrations of Humic Acids or Calcium Nitrate at Germination and Early Growth

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