ABSTRACT. Salinity is an important abiotic stress which can affect crop production in the world. One of the simplest methods for improving salinity tolerance of plants is seeds priming. This experiment was conducted to evaluate the effects of seeds priming with three solutions (KCl , NaCl and CaCl 2 ) in germination and later growth of three pepper (Capsicum annuum L.) cultivars: Beldi, Baklouti and Anaheim Chili. Seeds germination was conducted in a completely randomized design under seven salinity levels (0, 2, 4, 6, 8, 10 and 12 g L -1 ) at room temperature for primed and control seeds. Plants derived from these germinated seeds (control and primed) were transplanted and cultivated in a greenhouse for 4 months and were irrigated permanently with seven salinity levels (0, 2, 4, 6, 8, 10 and 12 g L -1). The results showed that salinity affected all parameters under study like total germination percentage and chlorophyll level (a and b). As well, proline content increased as response to increasing salinity. The plants derived and grown from primed seeds showed a considerable tolerance to salt stress and gave better results. In fact, priming improved the salt resistance of pepper owing to more chlorophyll and proline accumulation. These results suggest that seed priming induced possible physiological adjustments in pepper seeds, especially in the early stages of development, and could be used as a suitable tool for improving germination and growth characteristics under salt stress conditions.
Seed priming is a simple pre-germination method to improve seed performance and to attenuate the effects of stress exposure. The objective of this study was to determinate an optimal priming protocol for three pepper cultivars (Capsicum annuum L.): 'Beldi', 'Baklouti' and 'Anaheim Chili'. Seeds were primed with three solutions of NaCl, KCl and CaCl 2 (0, 10, 20 and 50 mM) for three different durations (12, 24 and 36h). Control seeds were soaked in distilled water for the same durations. After that, all seeds were kept to germinate in laboratory under normal light and controlled temperature. Results indicated that priming depends on concentration, duration and cultivar. The best combinations that we obtained were: KCl priming (10 mM, 36h) for 'Beldi' cultivar, CaCl 2 priming (10 mM, 36h) for 'Baklouti' cultivar and finally NaCl priming (50 mM, 24h) for 'Anaheim Chili' cultivar. Generally, priming had an effect on total germination percentage, mean germination time, germination index and the coefficient of velocity compared to control seeds. The beneficial effect of seed priming could be used for improving salt tolerance on germination and early seedling growth for pepper cultivar. Predobdelava semen pred kalitvijo je preprosta metoda za izboljšanje kalitve semen in zmanjšanje učinkov izpostavljenosti stresu. Predmet raziskave je bil izdelava optimalnega protokola predobdelave semen za tri sorte paprike (Capsicum annuum L.): 'Beldi', 'Baklouti' and 'Anaheim Chili'. Semena so bila pred kalitvijo obdelana z raztopinami NaCl, KCl in CaCl 2 (0, 10, 20 in 50 mM) v treh različnih trajanjih (12, 24 in 36h). Kontrolna semena so bila za enak čas namočena v destilirano vodo. Po predobdelavi je bil z vsemi semeni narejen kalitveni test v laboratoriju pri sobni svetlobi in nadzorovani temperaturi. Rezultati so pokazali, da je učinek predobdelave odvisen od koncentracije in vrste raztopin, trajanja predobdelave in sorte. Najboljša kalitev je bila dosežena pri predobdelavi s KCl (10 mM, 36h) za sorto 'Beldi', s CaCl 2 (10 mM, 36h) za sorto'Baklouti' in z NaCl (50 mM, 24h) za sorto 'Anaheim Chili'. V splošnem je imela predobdelva v primerjavi s kontrolo učinek na odstotek kalitve, povprečen čas kalitve, kalitveni indeks in koeficient hitrosti kalitve. Blagodejni učinek predobdelave semen bi lahko uporabljali za izboljšanje tolerance kalitve na slanost in hitrejšo zgodnjo rast sadik različnih sort paprike.
ABSTRACT. This study was conducted in order to evaluate the response of sesame seeds (Sesamum indicum L.) to organic or mineral fertilization. The effects of two fertilizers, which were calcium nitrate and humic acids were studied separately at germination and early seedlings growth. Different concentrations of humic acids (HA 0 : 0, HA 1 : 500, HA 2 : 1000 and HA 3 : 2000 mg L -1 ) or calcium nitrate (CaN 0 : 0, CaN 1 : 50, CaN 2 : 100 and CaN 3 : 200 mg L -1 ) were applied distinctly to the growth media. The experimental design was accomplished in a completely randomized block design with three replications. Germination measurements, seedlings length, fresh seedling weight, total chlorophylls and carotenoids, total soluble proteins and sugars were determined. Results showed that humic acids applied at 1000 mg L -1 or calcium nitrate applied at 100 mg L -1 reduced the mean germination time (3.5 and 3.61 days) and had the highest germination index (GI) and the highest coefficient of velocity (CV). The longest seedling was obtained at the concentrations HA 2 and CaN 2 (+22%). Total chlorophyll and carotenoids were significantly higher in seedlings receiving the HA 2 and CaN 2 and these treatments increased total soluble proteins content by 32%. Higher concentrations of humic acids or calcium nitrate HA 3 and CaN 3 delayed germination and enhanced proline and total soluble sugars, respectively, by 42% and 46%, compared to control. These preliminary results indicated that the use of fertilizers should be optimized and they can be transformed at high level to an abiotic stress menacing plant productivity. On the other hand, suitable concentrations of fertilizers can be used in the future as a remedy to improve growth under abiotic stress.
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