Prolific shoot regeneration of Astragalus cariensis Boiss

Erişen, Semiha; Yorgancılar, Mustafa; Atalay, Emine; Babaoğlu, Mehmet

doi:10.1007/s11240-009-9638-3

Cited by 23 publications

(17 citation statements)

References 10 publications

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“…But, it was observed that MS with 2.5 mg/l BAP alone was the most efficient treatment for multiple shoot induction on cotyledons than the combinations. On the contrary positive synergistic effect of cytokinins like BAP or Kn or TDZ or 2iP or zeatin in combination with an auxin like IAA, or NAA on in vitro shoot regeneration through organogenic differentiation has been reported by several workers (Shen et al 2007, Unda et al 2007, Erisen et al 2010, Ghimire et al 2010, Lin et al 2011, Wadl et al 2011, Mandal and Laxminarayana 2012, Perez-Jimenez et al 2012and Bhagya et al 2013. Superior effect of BAP for shoot multiplication has been reported among various medicinal plants of Asteraceae, like Tanacetum cinerariifolium (Hedayat et al 2009), Spilanthes acmella (Saritha and Naidu 2008), Launaea sarmentosa (Mahesh et al 2012).…”

Section: Resultsmentioning

confidence: 99%

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Nayak

Kalidass

2016

Plant Tissue Cult. & Biotech.

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Multiple shoots were induced on cotyledon explants of in vitro grown seedlings of Blepharispermum subsessile DC, cultured on MS medium supplemented with various combinations and concentrations of BAP, IBA and GA 3 . The highest regenerative response was observed on medium containing 2.5 mg/l BAP where shoot buds initiated after 12 days of inoculation and about 32 shoots were produced in 30 days time. Addition of GA 3 played a key role in leaf expansion and elongation of shoot buds. Addition of the auxin IBA to the induction medium resulted in more callus proliferation rather than shoot bud induction. The elongated shoots were transferred to root induction medium consisting of half strength MS supplemented with IAA, NAA and IBA. Highest rooting response (90%) was recorded in ½ MS supplemented with 1.0 mg/l IAA. Acclimatized plants were maintained in polybags with garden soil for future reintroduction program to their natural habitat.

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Section: Resultsmentioning

confidence: 99%

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Nayak

Kalidass

2016

Plant Tissue Cult. & Biotech.

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“…The failure of 2,4-D to induce shoot formation on petal explants of St. John's wort is consistent with published reports of its inhibitory effect on shoot regeneration from leaf and anther explants of this species (Pretto and Santarem 2000;Murch and Saxena 2002). Astragalus cariensis, callus generated on a medium with 2,4-D and kinetin, did not produce shoots (Erisen et al 2010). However, in the presence of high sucrose levels, 2,4-D in combination with BA induced embryogenic callus formation on carnation petals (Karami et al 2008).…”

Section: Shoot Regenerationmentioning

confidence: 92%

Plant regeneration from petal explants of Hypericum perforatum L

Palmer

Keller

2010

Plant Cell Tiss Organ Cult

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Hypericum perforatum L. (St. John's wort) produces a number of phytochemicals having medicinal, anti-microbial, anti-viral and anti-oxidative properties. Plant extracts are generally used for treatment of mild to medium cases of depression. Plant regeneration can be achieved in this species by in vitro culture of a variety of explants. However, there are no reports of regeneration from petal explants. In this report plant regeneration from petal explants of St. John's wort was evaluated. Petals of various ages were cultured on agarized Murashige and Skoog 1962 (MS) medium supplemented with auxin and cytokinin (kinetin), maintained in the dark and callus and shoot regeneration determined after 28 days. At an auxin to cytokinin ratio of 10:1, callus and shoot formation were induced by all levels of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA), while 2,4-dichlorophenoxyacetic acid (2,4-D) induced only callus formation. The optimum level of auxin for shoot regeneration was 1.0 and 0.1 mg/l kinetin, where the regeneration frequency was 100 percent for all three auxins. The highest number of shoots per explant (57.4 and 53.4) was obtained with IAA and IBA, respectively. In the absence of auxin, kinetin levels of 0.1 and 0.25 mg/l induce callus and shoot formation at low frequency but not at lower levels. Callus and shoot formation did not occur in the absence of growth regulators. Petal-derived shoots were successfully rooted on half-strength MS medium without a requirement for exogenous auxin and flowering plants were established under greenhouse conditions. From these results it can be concluded that auxin type is a critical factor for plant regeneration from petal explants of Hypericum perforatum and there is no absolute requirement for high levels of cytokinin.

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“…To our knowledge, although there are some previous reports on callus induction and shoot regeneration in some species of other Leguminosae family plants, to date no successful method has been reported for shoot regeneration from callus of S.alopecuroides L, [2,3,[9][10][11]13]. In these species, callus differentiation and shoot regeneration were determined by growth regulators [2][3][9][10]. In addition, other studies on Astragalus species also described various calli types that were formed during callus induction, associated with shoot regeneration.…”

Section: Introductionmentioning

confidence: 93%

“…Moreover, callus production is also a necessary step for obtaining protoplasts used in protoplast fusion, a useful tool in genetic improvement of vegetatively propagated Sophora's species for introducing useful genes or producing new crops [4]. To our knowledge, although there are some previous reports on callus induction and shoot regeneration in some species of other Leguminosae family plants, to date no successful method has been reported for shoot regeneration from callus of S.alopecuroides L, [2,3,[9][10][11]13]. In these species, callus differentiation and shoot regeneration were determined by growth regulators [2][3][9][10].…”

Section: Introductionmentioning

confidence: 99%

Plant Regeneration From Callus Cultures Derived From Mature Zygotic Embryos of Sophora Alopecuroides Linn. In Mongolia

Tsolmon¹,

Ganbat²,

Yu³

2018

Proc. Mong. Acad. Sci.

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The aim of this study is to determine the effect of hormones and selection of the most effective medium using callus cultures derived from mature zygotic embryos of Sophora alopecuroides Linn. for plant regeneration. After 8 weeks of culture, the highest callus induction medium (93.3%) was obtained on MS medium supplemented with 0.2 mglL Zeatin and 2.0 mg/L α-naphthaleneacetic acid (NAA). The best callus proliferation was observed on the same medium. Shoots regenerated at the highest frequency of 50.0% with 5.8 shoots when calli were cultured on MS medium with 2.0 mg/L BA. Therefore, this protocol provides a basis for future studies on genetic improvement and could be applied to large-scale multiplication systems for commercial nurseries of S.alopecuroides L.

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Prolific shoot regeneration of Astragalus cariensis Boiss

Cited by 23 publications

References 10 publications

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Plant regeneration from petal explants of Hypericum perforatum L

Plant Regeneration From Callus Cultures Derived From Mature Zygotic Embryos of Sophora Alopecuroides Linn. In Mongolia

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