Proliferative Classification of Intracranially Injected HER2-positive Breast Cancer Cell Lines

Kuroiwa, Yoshihiro; Nakayama, Jun; Adachi, Chihiro; Inoue, Takafumi; Watanabe, Shinya; Semba, Kentaro

doi:10.3390/cancers12071811

Cited by 23 publications

(32 citation statements)

References 78 publications

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“…These two approaches have resulted in luciferin analogues with improved characteristics for in vivo imaging, as discussed below. [15], [16], [18], [19], [20], [21], [24], [28], [29], [30], [31], [32], [33], [34], [35], [36], [ Miller et al developed CycLuc1, in which a pyrrolidine moiety is fused to the benzothiazole moiety of DLH2. The wavelength of CycLuc1 is 599 nm (Km = 0.10 and 1.06 μM in Table 2), which is longer than that of DLH2 due to electron donation from the fused N-hetero ring [75].…”

Section: Luciferin Analogues That Produce Nir Lightmentioning

confidence: 99%

“…Luciferin–luciferase reactions are also used for in vivo real-time monitoring of gene expression, cell numbers, and other biological events. Specifically, firefly BLI has been utilized for in vivo study in numerous mouse models [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. For instance, it has been used to quantify tumor cells [ 37 ] and monitor gene expression in transgenic mouse models [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

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How to Select Firefly Luciferin Analogues for In Vivo Imaging

Saito-Moriya

Nakayama²,

Kamiya

et al. 2021

IJMS

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Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly BLI technology and discuss the development of luciferin analogues for high-resolution in vivo BLI. In addition, we discuss the novel luciferin analogues TokeOni and seMpai, which show potential as high-sensitivity in vivo BLI reagents.

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Section: Luciferin Analogues That Produce Nir Lightmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

How to Select Firefly Luciferin Analogues for In Vivo Imaging

Saito-Moriya

Nakayama²,

Kamiya

et al. 2021

IJMS

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“…Bioluminescence imaging (BLI) is used to monitor the behavior of cells and molecules in animal studies [ 1 , 2 ]. In cancer research, the in vivo BLI system with luciferase is used for the quantification of tumor volume, monitoring gene expression and detection of cancer metastasis [ 3 , 4 , 5 , 6 , 7 , 8 ]. However, luminescence of visible light (450–600 nm) including D-luciferin’s wavelength (560 nm) is absorbed by tissues.…”

Section: Introductionmentioning

confidence: 99%

High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

Nakayama

Saito

Hayashi

et al. 2020

IJMS

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Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-infrared (NIR) luciferin analogues enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C15H16N3O2S) which has been synthesized as a luciferin analogue and has high luminescent abilities as same as AkaLumine. We demonstrated that seMpai BLI could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. Our findings suggest that the development of a novel mutated luciferase against seMpai may enable a highly sensitive BLI at the single-cell level without any background signal. Novel seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine.

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“…In addition, to promote the exchange of information on these valuable resources, the brain metastasis cell lines panel (BrMPanel) [ 58 ] and the metastasis map (MetMap) of human cancer cell lines [ 59 ] has been published as a useful public dataset. Cataloging the metastatic tropism and activity of cancer cell lines has reached a major turning point by these datasets [ 58 , 59 , 60 ]. Here, we describe a study of metastasis biology with gene expression profiling using highly metastatic breast cancer cell lines established by in vivo selection.…”

Section: Highly Metastatic Breast Cancer Cell Linesmentioning

confidence: 99%

The In Vivo Selection Method in Breast Cancer Metastasis

Nakayama¹,

Han

Kuroiwa

et al. 2021

IJMS

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Metastasis is a complex event in cancer progression and causes most deaths from cancer. Repeated transplantation of metastatic cancer cells derived from transplanted murine organs can be used to select the population of highly metastatic cancer cells; this method is called as in vivo selection. The in vivo selection method and highly metastatic cancer cell lines have contributed to reveal the molecular mechanisms of cancer metastasis. Here, we present an overview of the methodology for the in vivo selection method. Recent comparative analysis of the transplantation methods for metastasis have revealed the divergence of metastasis gene signatures. Even cancer cells that metastasize to the same organ show various metastatic cascades and gene expression patterns by changing the transplantation method for the in vivo selection. These findings suggest that the selection of metastasis models for the study of metastasis gene signatures has the potential to influence research results. The study of novel gene signatures that are identified from novel highly metastatic cell lines and patient-derived xenografts (PDXs) will be helpful for understanding the novel mechanisms of metastasis.

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Proliferative Classification of Intracranially Injected HER2-positive Breast Cancer Cell Lines

Cited by 23 publications

References 78 publications

How to Select Firefly Luciferin Analogues for In Vivo Imaging

How to Select Firefly Luciferin Analogues for In Vivo Imaging

High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

The In Vivo Selection Method in Breast Cancer Metastasis

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